SEVA-Sib list

The sibling (Sib) vectors and tools included in this section follow some—but not all—standardization criteria of the collection and do not have a SEVA code, and they named with the designation given by their creators. Yet, they include a large number of useful assetts which, in most cases, can be combined with bits and parts of the canonical collection at user’s will. Please note that information on these non-canonical vectors—kindly shared by a large number of contributors— is reproduced as provided by the authors, but it is not curated by the database managers. The SEVA-sib list includes e.g. a large collection of heterologous expression systems.

Name Resistance ORI Cargo-like GenBank # or seq Developer Reference
pSEVAT1Z1A Ap R6K thyA
complement, Zeta toxin
KJ686596 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVAD1Z1A Ap R6K dapA
complement, Zeta toxin
KJ686597 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVAT1K1A Ap R6K thyA
complement, Kid toxin
KJ686598 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVAD1K1A Ap R6K dapA
complement, Kid toxin
KJ686599 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVAT7Z1A Ap ColE2-P9 thyA
complement, Zeta toxin
KJ686600 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVAD7Z1A Ap ColE2-P9 dapA
complement, Zeta toxin
KJ686601 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVAT7K1A Ap ColE2-P9 thyA
complement, Kid toxin
KJ686602 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVAD7K1A Ap ColE2-P9 dapA
complement, Kid toxin
KJ686603 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVA12DKR-A Km RK2 dapA targeting
homology arms, pi-A rep gene, Kis antitoxin, FRT-Km-FRT
KJ686604 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVA12DKR-E Km RK2 dapA
targeting homology arms, pi-E rep gene, Kis antitoxin, FRT-Km-FRT
KJ686605 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVA12DKR-J Km RK2 dapA targeting
homology arms, pi-J rep gene, Kis antitoxin, FRT-Km-FRT
KJ686606 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVA12TEC-B Km RK2 thyA
targeting homology arms, repA-B rep gene, Epsilon antitoxin, FRT-Km-FRT
KJ686607 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVA12TEC-I Km RK2 thyA
targeting homology arms, repA-I rep gene, Epsilon antitoxin, FRT-Km-FRT
KJ686608 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVA12TEC-K Km RK2 thyA
targeting homology arms, repA-J rep gene, Epsilon antitoxin, FRT-Km-FRT
KJ686609 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVA3b61 Cm pUC-pTHT15 Shuttle vector for use in E. coli / B. subtilis KJ686616 Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pSEVA3b67Rb Cm pUC-pTHT15 Shuttle vector with constitutive mRFP1, compatible as
reporter for E.
coli
/ B.
subtilis
Ellis´lab Wright O. et al. ACS Synthetic Biology. 2015
pBAMD1-2 Ap, Km R6K mini-Tn5 transposon KM403113 de Lorenzo´s lab Martínez-garcía E. e al. Frontiers in bioengineering and
Biotehnology. 2014
pBAMD1-4 Ap, Sm/Sp R6K mini-Tn5 transposon KM403114 de Lorenzo´s lab Martínez-garcía E. e al. Frontiers in bioengineering and
Biotehnology. 2014
pBAMD1-6 Ap, Gm R6K mini-Tn5 transposon KM403115 de Lorenzo´s lab Martínez-garcía E. e al. Frontiers in bioengineering and
Biotehnology. 2014
pBG28 Gm, Km R6K mini-Tn7 transposon, translational coupler , synthetic promoter 14a KR872418 Blank’s Lab Zobel et al., ACS Synthetic Biology. 2015
pTn7-M Gm, Km R6K mini-Tn7 transposon, MCS KR920750 de Lorenzo´s lab Zobel et al., ACS Synthetic Biology. 2015
pBG37 Gm, Km R6K mini-Tn7 transposon, translational coupler, synthetic promoter 14c KT192132 Blank’s lab Zobel et al., ACS Synthetic Biology. 2015
pBG51 Gm, Km R6K mini-Tn7 transposon, translational coupler, synthetic promoter 14d KT192133 Blank’s lab Zobel et al., ACS Synthetic Biology. 2015
pBG42 Gm, Km R6K mini-Tn7 transposon, translational coupler, synthetic promoter 14g KT192134 Blank’s lab Zobel et al., ACS Synthetic Biology. 2015
pM13 Gm, Km R6K mini-Tn7 transposon, SEVA cargo: 13 KT192135 de Lorenzo´s lab Zobel et al., ACS Synthetic Biology. 2015
pBG Gm, Km R6K mini-Tn7 transposon, translational coupler KT192136 de Lorenzo´s lab Zobel et al., ACS Synthetic Biology. 2015
pBG13 Gm, Km R6K mini-Tn7 transposon, translational coupler, SEVA cargo: 13 KT192137 de Lorenzo´s lab Zobel et al., ACS Synthetic Biology. 2015
pBG17 Gm, Km R6K mini-Tn7 transposon, translational coupler, synthetic promoter 14e KT192138 Blank’s lab Zobel et al., ACS Synthetic Biology. 2015
pBG25 Gm, Km R6K mini-Tn7 transposon, translational coupler, synthetic promoter 14f KT192139 Blank’s lab Zobel et al., ACS Synthetic Biology. 2015
pBG35 Gm, Km R6K mini-Tn7 transposon, translational coupler, synthetic promoter 14b KT192141 Blank’s lab Zobel et al., ACS Synthetic Biology. 2015
pM13 Km, Gm R6K mini-Tn7 transposon, SEVA cargo: 13 KT192135.3 Blank’s Lab Zobel et al., ACS Synthetic Biology. 2015
pBG34 Km, Gm R6K mini-Tn7 transposon, translational coupler, synthetic
promoter 34
Blank’s Lab Zobel et al., ACS Synthetic Biology. 2015
pSEVA221-MAGE Km RK2 Csaba Pal´s lab Nyerges Á. et al. PNAS. 2016
pSEVA231-MAGE Km pBBR1 pSEVA231-MAGE.gbk Csaba Pal´s lab Nyerges Á. et al. PNAS. 2016
pPS36 Sm/Sp pRO1600/ColE1 xylS-Pm-USER cassette Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS37 Sm/Sp pRO1600/ColE1 araC-ParaB-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS38 Sm/Sp pRO1600/ColE1 lacI-PT7-USER cassette Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS39 Sm/Sp pRO1600/ColE1 rhaSrhaR-PrhaB-USER
cassette
pPS39.gb Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS42 Sm/Sp pRO1600/ColE1 alkS-PalkB-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS43 Sm/Sp pRO1600/ColE1 nahR-Psal-USER cassette Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS44 Sm/Sp pRO1600/ColE1 lacI-PlacUV5-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS50 Sm/Sp pRO1600/ColE1 rhaTrhaSrhaR-PrhaB-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS51 Sm/Sp pRO1600/ColE1 araE-araC-ParaB-USER cassette Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS1 Gm pBBR1 xylS-Pm-USER cassette Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS2 Gm pBBR1 araC-ParaB-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS3 Gm pBBR1 lacI-PT7-USER cassette Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS4 Gm pBBR1 rhaSrhaR-PrhaB-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS6 Gm pBBR1 alkS-PalkB-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS7 Gm pBBR1 nahR-Psal-USER cassette Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS8 Gm pBBR1 lacI-PlacUV5-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS10 Gm pBBR1 rhaTrhaSrhaR-PrhaB-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS11 Gm pBBR1 araEaraC-ParaB-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS23 Km RK2 xylS-Pm-USER cassette Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS24 Km RK2 araC-ParaB-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS25 Km RK2 lacI-PT7-USER cassette Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS26 Km RK2 rhaSrhaR-PrhaB-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS28 Km RK2 alkS-PalkB-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS29 Km RK2 nahR-Psal-USER cassette Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS30 Km RK2 lacI-PlacUV5-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS32 Km RK2 rhaTrhaSrhaR-PrhaB-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS33 Km RK2 araEaraC-ParaB-USER
cassette
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS52 Sm/Sp pRO1600/ColE1 xylS-Pm–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS53 Sm/Sp pRO1600/ColE1 araC-ParaB—>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS54 Sm/Sp pRO1600/ColE1 lacI-PT7–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS55 Sm/Sp pRO1600/ColE1 rhaSrhaR-PrhaB–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS58 Sm/Sp pRO1600/ColE1 alkS-PalkB–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS59 Sm/Sp pRO1600/ColE1 nahR-Psal–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS60 Sm/Sp pRO1600/ColE1 lacI-PlacUV5–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS80 Sm/Sp pRO1600/ColE1 rhaTrhaSrhaR-PrhaB–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS81 Sm/Sp pRO1600/ColE1 araEaraC-ParaB–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS66 Sm/Sp pRO1600/ColE1 xylS-Pm–>mcherry Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS84 Gm pBBR1 xylS-Pm–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS85 Gm pBBR1 araC-ParaB–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS86 Gm pBBR1 lacI-PT7–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS87 Gm pBBR1 rhaSrhaR-PrhaB–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS89 Gm pBBR1 alkS-PalkB–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS90 Gm pBBR1 nahR-Psal–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS91 Gm pBBR1 lacI-PlacUV5–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS93 Gm pBBR1 rhaTrhaSrhaR-PrhaB–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS94 Gm pBBR1 araEaraC-ParaB–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS95 Km RK2 xylS-Pm–>mcherry Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pPS131 Gm RSF1010 rhaSrhaR-PrhaB–>GFP(mut3) Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pSIJ218 Km R6K pEMG derived, T7 polymerase flanked by homologous regions
for insertion after glmS
gene
Nielsen´s lab Calero P. et al ACS Synthetic Biology. 2016
pBSd141 Ap pRO1600/ColE1 mRFP1 (const. Expressed); IIs-sites
«destination»
Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSd191 Ap pBR322/ROP mRFP1 (const. Expressed); IIs-sites
«destination»
Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc241ble Km+Pm* pRO1600/ColE1 MCS-default; PmR, IIs-sites «cargo» Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc241cat Km+Cm* pRO1600/ColE1 MCS-default; CmR, IIs-sites «cargo» Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc241mls Km+MLS* pRO1600/ColE1 MCS-default; MLSR, IIs-sites «cargo» Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc241spec Km+Sm/Sp* pRO1600/ColE1 MCS-default; SpR, IIs-sites «cargo» Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc241tet-short Km+Tc* pRO1600/ColE1 MCS-default; TcR, IIs-sites «cargo» Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc241zeo Km+Zc* pRO1600/ColE1 MCS-default; ZcR, IIs-sites «cargo» Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc243ble Km+Pm* pRO1600/ColE1 lacZα-pUC18;
Pm
R,
IIs-sites «cargo»
Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc243cat Km+Cm* pRO1600/ColE1 lacZα-pUC18;
Cm
R,
IIs-sites «cargo»
Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc243mls Km+MLS* pRO1600/ColE1 lacZα-pUC18;
MLS
R,
IIs-sites «cargo»
Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc243spec Km+Sm/Sp* pRO1600/ColE1 lacZα-pUC18;
Sp
R,
IIs-sites «cargo»
Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc243tet-short Km+Tc* pRO1600/ColE1 lacZα-pUC18;
Tc
R,
IIs-sites «cargo»
Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc243zeo Km+Zc* pRO1600/ColE1 lacZα-pUC18;
Zc
R,
IIs-sites «cargo»
Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc291kan Km+Km* pBR322/ROP MCS-default; KmR, IIs-sites «cargo» Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc293kan Km+Km* pBR322/ROP lacZα-pUC18;
Km
R,
IIs-sites «cargo»
Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc391kan Cm+Km* pBR322/ROP MCS-default; CmR, IIs-sites «cargo» Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc393kan Cm+Km* pBR322/ROP lacZα-pUC18;
Cm
R,
IIs-sites «cargo»
Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc241 Km pRO1600/ColE1 MCS-default; IIs-sites «cargo» Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc243 Km pRO1600/ColE1 lacZα-pUC18;
IIs-sites «cargo»
Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc291 Km pBR322/ROP MCS-default; IIs-sites «cargo» Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc293 Km pBR322/ROP lacZα-pUC18;
IIs-sites «cargo»
Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc391 Cm pBR322/ROP MCS-default; IIs-sites «cargo» Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pBSc393 Cm pBR322/ROP lacZα-pUC18 Mascher´s lab Radeck J. et al. Scientific Reports. 2017
pSEVA224-AEB Km RK2 lacIqPtrc Müller´s lab Jahn M. et al. Microbial Cell Factories. 2016
pSEVA234-AEB Km RK2 lacIqPtrc Müller´s lab Jahn M. et al. Microbial Cell Factories. 2016
pSEVA244-AEB Km RK2 lacIqPtrc Müller´s lab Jahn M. et al. Microbial Cell Factories. 2016
pSEVA254-AEB Km RK2 lacIqPtrc Müller´s lab Jahn M. et al. Microbial Cell Factories. 2016
pSEVA264-AEB Km RK2 lacIqPtrc Müller´s lab Jahn M. et al. Microbial Cell Factories. 2016
pSEVA274-AEB Km RK2 lacIqPtrc Müller´s lab Jahn M. et al. Microbial Cell Factories. 2016
pSEVA284-AEB Km RK2 lacIqPtrc Müller´s lab Jahn M. et al. Microbial Cell Factories. 2016
pABCa Gm pMlb / p15A syn Ter1; Design type A KY031724 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pABCb Sm/Sp p42b / pMB1 syn Ter2; Design type A KY031725 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pABCc Km p42d / pSC101* syn Ter3; Design type A KY031726 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pABCc-mob Km p42d / pSC101* syn Ter3; mob; Design type B KY031727 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pABC1 Sm/Sp p42b / pMB1 syn Ter1; Design type A KY031728 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pABC1-loxA Sm/Sp p42b / pMB1 syn Ter1-loxL; mob-loxR; Design
type C1
KY031729 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pABC1-loxB Sm/Sp p42b / pMB1 syn Ter1-loxL; mob-loxR-rrnBT2; Design
type C2
KY031730 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pABC1-sT2 Sm/Sp p42b / pMB1 syn Ter2; Design type A KY031731 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pABC1-sT3 Sm/Sp p42b / pMB1 syn Ter3; Design type A KY031732 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pABC2 Gm pMla / p15A syn Ter2; Design type A KY031733 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pABC3 hygromycin pTi / pSC101* syn Ter3; Design type A KY031734 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pABC4 Sm/Sp p42e / pMB1 syn Ter1; Design type A KY031735 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pABC5 Sm/Sp p42f1 / pMB1 syn Ter2; Design type A KY031736 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pABC6 Sm/Sp p42f2 / pMB1 syn Ter2; Design type A KY031737 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLoriV-42b Km oriVp42b (R. etli) KY031698 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLoriV-42d Km oriVp42d (R. etli) KY031699 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLoriV-42e Km oriVp42e (R. etli) KY031700 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLoriV-42f1 Km oriVp42frepA1B1C1 (R. etli) KY031701 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLoriV-42f2 Km oriVp42frepA2B2C2 (R. etli) KY031702 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLoriV-Mla Km oriVpMla (M. loti) KY031703 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLoriV-Mlb Km oriVpMlb (M. loti) KY031704 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLoriV-Ti Km oriVpTi (A. tumefaciens) KY031705 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLsynTer-1 Km pMB1 syn Ter1-MCS KY031706 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLsynTer-2 Km pMB1 syn Ter2-MCS KY031707 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLsynTer-3 Km pMB1 syn Ter3-MCS KY031708 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLsynTer-1-lox Km pMB1 syn Ter1-MCS-loxL KY031709 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLsynTer-2-lox Km pMB1 syn Ter2-MCS-loxL KY031710 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLsynTer-3-lox Km pMB1 syn Ter3-MCS-loxL KY031711 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLoriT-1 Km pMB1 mob KY031712 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLoriT-1-lox Km pMB1 mob-loxR KY031713 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLoriT-1-loxT Km pMB1 mob-loxR-rrnBT2 KY031714 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLAR-Km Km pMB1 KY031715 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLAR-Spec Sm/Sp, Km pMB1 KY031716 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLAR-Tc Tet, Km pMB1 KY031717 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLAR-Hyg Hygromycin, Km pMB1 KY031718 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLAR-Tmp Trimethoprim, Km pMB1 KY031719 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pLAR-Gm Gm, Km pMB1 KY031720 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pK18mob2 Km pMB1 KY031721 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pACYC184 Tet p15A KY031722 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pXG-10 Cm pSC101* KY031723 Becker´s lab Döhlemann J. et al.
ACS Synthetic Biology. 2017
pRK99 Km, Gm R6K MN626472 Prieto´s lab
pRK100 Km RK2 MN626473 Prieto´s lab
pRK101 Km pRO1600/ColE1 MN626474 Prieto´s lab
pRK102 Gm RK2 MN626475 Prieto´s lab
pRK177 Tet pRO1600/ColE1 MN626476 Prieto´s lab
pBAM1-GFP Ap,Km R6K mini-Tn5 transposon de Lorenzo´s lab
pSEVAb127 Ap RK2 Bba_J23105-GFP MW476665.1 Martins dos Santos´ lab
pSEVAb137 Ap pBBR1 Bba_J23105-GFP MW476666.1 Martins dos Santos´ lab
pSEVAb147 Ap pRO1600/ColE1 Bba_J23105-GFP MW476667.1 Martins dos Santos´ lab
pSEVAb157 Ap RSF1010 Bba_J23105-GFP MW476668.1 Martins dos Santos´ lab
pSEVAb227 Km RK2 Bba_J23105-GFP MW476669.1 Martins dos Santos´ lab
pSEVAb237 Km pBBR1 Bba_J23105-GFP MW476670.1 Martins dos Santos´ lab
pSEVAb247 Km pRO1600/ColE1 Bba_J23105-GFP MW476671.1 Martins dos Santos´ lab
pSEVAb257 Km RSF1010 Bba_J23105-GFP MW476672.1 Martins dos Santos´ lab
pSEVAb427 Sm/Sp RK2 Bba_J23105-GFP MW476673.1 Martins dos Santos´ lab
pSEVAb437 Sm/Sp pBBR1 Bba_J23105-GFP MW296039.1 Martins dos Santos´ lab
pSEVAb447 Sm/Sp pRO1600/ColE1 Bba_J23105-GFP MW476674.1 Martins dos Santos´ lab
pSEVAb457 Sm/Sp RSF1010 Bba_J23105-GFP MW476675.1 Martins dos Santos´ lab
pSEVAb627 Gm RK2 Bba_J23105-GFP MW476676.1 Martins dos Santos´ lab
pSEVAb637 Gm pBBR1 Bba_J23105-GFP MW476677.1 Martins dos Santos´ lab
pSEVAb647 Gm pRO1600/ColE1 Bba_J23105-GFP MW476678.1 Martins dos Santos´ lab
pSEVAb657 Gm RSF1010 Bba_J23105-GFP MW476679.1 Martins dos Santos´ lab
pSEVA251-mVenus Km RSF1010 mVenus Luis Lopez Maury
pSEVA251-turquoise Km RSF1010 turquoise Luis Lopez Maury
pSEVA451-mVenus Sm/Sp RSF1010 mVenus Luis Lopez Maury
pSEVA451-turquoise Sm/Sp RSF1010 turquoise Luis Lopez Maury
pSEVA221::Amer-F1 Km RK2 Amer-F1; NOT gate OP271959 de Lorenzo´s lab
pSEVA221::AmtR-A1 Km RK2 AmtR-A1; NOT gate OP254164 de Lorenzo´s lab
pSEVA221::BetI-E1 Km RK2 BetI-E1; NOT gate OP254165 de Lorenzo´s lab
pSEVA221::BM3R1-B1 Km RK2 BM3R1-B1; NOT gate OP254166 de Lorenzo´s lab
pSEVA221::BM3R1-B2 Km RK2 BM3R1-B2; NOT gate OP254167 de Lorenzo´s lab
pSEVA221::BM3R1-B3 Km RK2 BM3R1-B3; NOT gate OP254168 de Lorenzo´s lab
pSEVA221::HIyIIR-H1 Km RK2 HIyIIR-H1; NOT gate OP254169 de Lorenzo´s lab
pSEVA221::lcaRA-I1 Km RK2 lcaRA-I1; NOT gate OP254170 de Lorenzo´s lab
pSEVA221::LitR-L1 Km RK2 LitR-L1; NOT gate OP254171 de Lorenzo´s lab
pSEVA221::LmrA-N1 Km RK2 LmrA-N1; NOT gate OP254172 de Lorenzo´s lab
pSEVA221::PhIF-P1 Km RK2 PhIF-P1; NOT gate OP254173 de Lorenzo´s lab
pSEVA221::PhIF-P2 Km RK2 PhIF-P2; NOT gate OP254174 de Lorenzo´s lab
pSEVA221::PhIF-P3 Km RK2 PhIF-P3; NOT gate OP254175 de Lorenzo´s lab
pSEVA221::PsrA-R1 Km RK2 PsrA-R1; NOT gate OP254176 de Lorenzo´s lab
pSEVA221::QacR-Q1 Km RK2 QacR-Q1; NOT gate OP254177 de Lorenzo´s lab
pSEVA221::QacR-Q2 Km RK2 QacR-Q2; NOT gate OP254178 de Lorenzo´s lab
pSEVA221::SrpR-S1 Km RK2 SrpR-S1; NOT gate OP254179 de Lorenzo´s lab
pSEVA221::SrpR-S2 Km RK2 SrpR-S2; NOT gate OP254180 de Lorenzo´s lab
pSEVA221::SrpR-S3 Km RK2 SrpR-S3; NOT gate OP254181 de Lorenzo´s lab
pSEVA221::SrpR-S4 Km RK2 SrpR-S4; NOT gate OP254182 de Lorenzo´s lab
pSEVA221::NOR-SrpR-S1 Km RK2 NOR-SrpR-S1; NOR gate OP254183 de Lorenzo´s lab
pSEVA221::1201 Km RK2 Empty plasmid for autofluorescence de Lorenzo´s lab
pSEVA221::1717 Km RK2 RPU standard plasmid for standardization de Lorenzo´s lab
pSEVA221::1818 Km RK2 pTac activity plasmid for promoter activity de Lorenzo´s lab
pSEVA221::1718 Km RK2 Inducer Characterization – IPTG de Lorenzo´s lab
pSEVA221::1719 Km RK2 Inducer Characterization – aTc de Lorenzo´s lab
pSEVA221::1720 Km RK2 Inducer Characterization – arabinose de Lorenzo´s lab
pSEVA1MIN11 Ap Min variant R6K MCS-default pSEVA1MIN11.gb Daniel Daley & Morten Norholm´s lab Cumming AJ. et al. ACS Synthetic
Biology. 2022
pSEVA1MIN21 Ap Min variant RK2 MCS-default pSEVA1MIN21.gb Daniel Daley & Morten Norholm´s lab Cumming AJ. et al. ACS Synthetic
Biology. 2022
pSEVA1MIN31 Ap Min variant pBBR1 MCS-default pSEVA1MIN31.gb Daniel Daley & Morten Norholm´s lab Cumming AJ. et al. ACS Synthetic
Biology. 2022
pSEVA1MIN41 Ap Min variant pRO1600/ColE1 MCS-default pSEVA1MIN41.gb Daniel Daley & Morten Norholm´s lab Cumming AJ. et al. ACS Synthetic
Biology. 2022
pSEVA1MIN51 Ap Min variant RFS1010 MCS-default pSEVA1MIN51.gb Daniel Daley & Morten Norholm´s lab Cumming AJ. et al. ACS Synthetic
Biology. 2022
pSEVA1MIN61 Ap Min variant p15A MCS-default pSEVA1MIN61.gb Daniel Daley & Morten Norholm´s lab Cumming AJ. et al. ACS Synthetic
Biology. 2022
pSEVA1MIN81 Ap Min variant pUC MCS-default pSEVA1MIN81.gb Daniel Daley & Morten Norholm´s lab Cumming AJ. et al. ACS Synthetic
Biology. 2022
pSEVA1MIN91 Ap Min variant pBR322/ROP MCS-default pSEVA1MIN91.gb Daniel Daley & Morten Norholm´s lab Cumming AJ. et al. ACS Synthetic
Biology. 2022
pS2313B Km pBBR1 PEM7 –> mBlue2 pS2313B.gb Pablo I. Nikel´s lab
pS2313O Km pBBR1 PEM7 –> mOrange2 pS2313O.gb Pablo I. Nikel´s lab
pBLAM1-2 Ap,Km R6K mariner mini-transposon pBLAM1-2.gb Goñi-Moreno´s lab
pBLAM1-4 Ap, Sm/Sp R6K mariner mini-transposon pBLAM1-4.gb Goñi-Moreno´s lab
pBLAM1-6 Ap, Gm R6K mariner mini-transposon pBLAM1-6.gb Goñi-Moreno´s lab
pSEVA421-Cas9tracrRNA Sm/Sp RK2 Constitutive expresion of Cas9-tracrRNA pSEVA421-Cas9-tracrRNA.gbk de Lorenzo´s lab Aparicio T. et al. Biotech. J, 2018
pSEVA658-ssr Gm RSF1010 Inducible expression of Ssr pSEVA658-ssr.gbk de Lorenzo´s lab Aparicio T. et al. Biotech. J, 2016
pSEVA6511-GIIi Gm RSF1010 chnR-PchnB –> Ll.LtrB – Ll.LtrB group II intron de Lorenzo´s lab Velazquez E. et al. ACS Synth. Biol. 2021.
pS23Pm::GFP Km pBBR1 Pm–> msfGFP Pablo I. Nikel´s lab Volke DC. et al. Microb. Biotech. 2020
pS628SRED Gm RK2 xylS-Pm–> I-sceI — P14g(BCD2) –> rfp Pablo I. Nikel´s lab Volke DC. et al. Metab. Eng. Commun. 2020.
pS228SRFP Km RK2 xylS-Pm–> I-sceI — P14g(BCD2) –> rfp Pablo I. Nikel´s lab Volke DC. et al. Metab. Eng. Commun. 2020.
pQURE6・H Gm Conditionally replicating RK2 xylS-Pm–> I-sceI — P14g –> rfp Pablo I. Nikel´s lab Volke DC. et al. Metab. Eng. Commun. 2020.
pTn7-xylS Gm, Km R6K Pablo I. Nikel´s lab Volke DC. et al. Microb. Biotech. 2020
pTn7-xylS-Nia Gm, Km R6K Pablo I. Nikel´s lab Volke DC. et al. Microb. Biotech. 2020
pSNW2 Km R6K P14g(BCD2) –> msfGFP Pablo I. Nikel´s lab Volke DC. et al. Metab. Eng. Commun. 2020.
pSNW4 Sm/Sp R6K P14g(BCD2) –> msfGFP Pablo I. Nikel´s lab Volke DC. et al. Metab. Eng. Commun. 2020.
pSNW5 Tet R6K P14g(BCD2) –> msfGFP Pablo I. Nikel´s lab Volke DC. et al. Metab. Eng. Commun. 2020.
pSNW6 Gm R6K P14g(BCD2) –> msfGFP Pablo I. Nikel´s lab Volke DC. et al. Metab. Eng. Commun. 2020.
pGNW2・LPG Km R6K P14g(BCD2) –> msfGFP Pablo I. Nikel´s lab Wirth NT. et al. Microb. Biotech. 2020.
pS448・CsR Sm/Sp pRO1600/ColE1 Pm–> cas9 — PEM7 –> sgRNA Pablo I. Nikel´s lab Wirth NT. et al. Microb. Biotech. 2020.