SEVA-Sib list
The sibling (Sib) vectors and tools included in this section follow some—but not all—standardization criteria of the collection and do not have a SEVA code, and they named with the designation given by their creators. Yet, they include a large number of useful assetts which, in most cases, can be combined with bits and parts of the canonical collection at user’s will. Please note that information on these non-canonical vectors—kindly shared by a large number of contributors— is reproduced as provided by the authors, but it is not curated by the database managers. The SEVA-sib list includes e.g. a large collection of heterologous expression systems.
Name | Resistance | ORI | Cargo-like | GenBank # or seq | Developer | Reference |
pSEVAT1Z1A | Ap | R6K | thyA complement, Zeta toxin |
KJ686596 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVAD1Z1A | Ap | R6K | dapA complement, Zeta toxin |
KJ686597 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVAT1K1A | Ap | R6K | thyA complement, Kid toxin |
KJ686598 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVAD1K1A | Ap | R6K | dapA complement, Kid toxin |
KJ686599 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVAT7Z1A | Ap | ColE2-P9 | thyA complement, Zeta toxin |
KJ686600 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVAD7Z1A | Ap | ColE2-P9 | dapA complement, Zeta toxin |
KJ686601 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVAT7K1A | Ap | ColE2-P9 | thyA complement, Kid toxin |
KJ686602 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVAD7K1A | Ap | ColE2-P9 | dapA complement, Kid toxin |
KJ686603 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVA12DKR-A | Km | RK2 | dapA targeting homology arms, pi-A rep gene, Kis antitoxin, FRT-Km-FRT |
KJ686604 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVA12DKR-E | Km | RK2 | dapA targeting homology arms, pi-E rep gene, Kis antitoxin, FRT-Km-FRT |
KJ686605 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVA12DKR-J | Km | RK2 | dapA targeting homology arms, pi-J rep gene, Kis antitoxin, FRT-Km-FRT |
KJ686606 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVA12TEC-B | Km | RK2 | thyA targeting homology arms, repA-B rep gene, Epsilon antitoxin, FRT-Km-FRT |
KJ686607 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVA12TEC-I | Km | RK2 | thyA targeting homology arms, repA-I rep gene, Epsilon antitoxin, FRT-Km-FRT |
KJ686608 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVA12TEC-K | Km | RK2 | thyA targeting homology arms, repA-J rep gene, Epsilon antitoxin, FRT-Km-FRT |
KJ686609 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVA3b61 | Cm | pUC-pTHT15 | Shuttle vector for use in E. coli / B. subtilis | KJ686616 | Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 |
pSEVA3b67Rb | Cm | pUC-pTHT15 | Shuttle vector with constitutive mRFP1, compatible as reporter for E. coli / B. subtilis |
Ellis´lab | Wright O. et al. ACS Synthetic Biology. 2015 | |
pBAMD1-2 | Ap, Km | R6K | mini-Tn5 transposon | KM403113 | de Lorenzo´s lab | Martínez-garcía E. e al. Frontiers in bioengineering and Biotehnology. 2014 |
pBAMD1-4 | Ap, Sm/Sp | R6K | mini-Tn5 transposon | KM403114 | de Lorenzo´s lab | Martínez-garcía E. e al. Frontiers in bioengineering and Biotehnology. 2014 |
pBAMD1-6 | Ap, Gm | R6K | mini-Tn5 transposon | KM403115 | de Lorenzo´s lab | Martínez-garcía E. e al. Frontiers in bioengineering and Biotehnology. 2014 |
pBG28 | Gm, Km | R6K | mini-Tn7 transposon, translational coupler , synthetic promoter 14a | KR872418 | Blank’s Lab | Zobel et al., ACS Synthetic Biology. 2015 |
pTn7-M | Gm, Km | R6K | mini-Tn7 transposon, MCS | KR920750 | de Lorenzo´s lab | Zobel et al., ACS Synthetic Biology. 2015 |
pBG37 | Gm, Km | R6K | mini-Tn7 transposon, translational coupler, synthetic promoter 14c | KT192132 | Blank’s lab | Zobel et al., ACS Synthetic Biology. 2015 |
pBG51 | Gm, Km | R6K | mini-Tn7 transposon, translational coupler, synthetic promoter 14d | KT192133 | Blank’s lab | Zobel et al., ACS Synthetic Biology. 2015 |
pBG42 | Gm, Km | R6K | mini-Tn7 transposon, translational coupler, synthetic promoter 14g | KT192134 | Blank’s lab | Zobel et al., ACS Synthetic Biology. 2015 |
pM13 | Gm, Km | R6K | mini-Tn7 transposon, SEVA cargo: 13 | KT192135 | de Lorenzo´s lab | Zobel et al., ACS Synthetic Biology. 2015 |
pBG | Gm, Km | R6K | mini-Tn7 transposon, translational coupler | KT192136 | de Lorenzo´s lab | Zobel et al., ACS Synthetic Biology. 2015 |
pBG13 | Gm, Km | R6K | mini-Tn7 transposon, translational coupler, SEVA cargo: 13 | KT192137 | de Lorenzo´s lab | Zobel et al., ACS Synthetic Biology. 2015 |
pBG17 | Gm, Km | R6K | mini-Tn7 transposon, translational coupler, synthetic promoter 14e | KT192138 | Blank’s lab | Zobel et al., ACS Synthetic Biology. 2015 |
pBG25 | Gm, Km | R6K | mini-Tn7 transposon, translational coupler, synthetic promoter 14f | KT192139 | Blank’s lab | Zobel et al., ACS Synthetic Biology. 2015 |
pBG35 | Gm, Km | R6K | mini-Tn7 transposon, translational coupler, synthetic promoter 14b | KT192141 | Blank’s lab | Zobel et al., ACS Synthetic Biology. 2015 |
pM13 | Km, Gm | R6K | mini-Tn7 transposon, SEVA cargo: 13 | KT192135.3 | Blank’s Lab | Zobel et al., ACS Synthetic Biology. 2015 |
pBG34 | Km, Gm | R6K | mini-Tn7 transposon, translational coupler, synthetic promoter 34 |
Blank’s Lab | Zobel et al., ACS Synthetic Biology. 2015 | |
pSEVA221-MAGE | Km | RK2 | Csaba Pal´s lab | Nyerges Á. et al. PNAS. 2016 | ||
pSEVA231-MAGE | Km | pBBR1 | pSEVA231-MAGE.gbk | Csaba Pal´s lab | Nyerges Á. et al. PNAS. 2016 | |
pPS36 | Sm/Sp | pRO1600/ColE1 | xylS-Pm-USER cassette | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS37 | Sm/Sp | pRO1600/ColE1 | araC-ParaB-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS38 | Sm/Sp | pRO1600/ColE1 | lacI-PT7-USER cassette | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS39 | Sm/Sp | pRO1600/ColE1 | rhaS–rhaR-PrhaB-USER cassette |
pPS39.gb | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 |
pPS42 | Sm/Sp | pRO1600/ColE1 | alkS-PalkB-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS43 | Sm/Sp | pRO1600/ColE1 | nahR-Psal-USER cassette | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS44 | Sm/Sp | pRO1600/ColE1 | lacI-PlacUV5-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS50 | Sm/Sp | pRO1600/ColE1 | rhaT–rhaS–rhaR-PrhaB-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS51 | Sm/Sp | pRO1600/ColE1 | araE-araC-ParaB-USER cassette | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS1 | Gm | pBBR1 | xylS-Pm-USER cassette | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS2 | Gm | pBBR1 | araC-ParaB-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS3 | Gm | pBBR1 | lacI-PT7-USER cassette | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS4 | Gm | pBBR1 | rhaS–rhaR-PrhaB-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS6 | Gm | pBBR1 | alkS-PalkB-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS7 | Gm | pBBR1 | nahR-Psal-USER cassette | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS8 | Gm | pBBR1 | lacI-PlacUV5-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS10 | Gm | pBBR1 | rhaT–rhaS–rhaR-PrhaB-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS11 | Gm | pBBR1 | araE–araC-ParaB-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS23 | Km | RK2 | xylS-Pm-USER cassette | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS24 | Km | RK2 | araC-ParaB-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS25 | Km | RK2 | lacI-PT7-USER cassette | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS26 | Km | RK2 | rhaS–rhaR-PrhaB-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS28 | Km | RK2 | alkS-PalkB-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS29 | Km | RK2 | nahR-Psal-USER cassette | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS30 | Km | RK2 | lacI-PlacUV5-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS32 | Km | RK2 | rhaT–rhaS–rhaR-PrhaB-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS33 | Km | RK2 | araE–araC-ParaB-USER cassette |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS52 | Sm/Sp | pRO1600/ColE1 | xylS-Pm–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS53 | Sm/Sp | pRO1600/ColE1 | araC-ParaB—>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS54 | Sm/Sp | pRO1600/ColE1 | lacI-PT7–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS55 | Sm/Sp | pRO1600/ColE1 | rhaS–rhaR-PrhaB–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS58 | Sm/Sp | pRO1600/ColE1 | alkS-PalkB–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS59 | Sm/Sp | pRO1600/ColE1 | nahR-Psal–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS60 | Sm/Sp | pRO1600/ColE1 | lacI-PlacUV5–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS80 | Sm/Sp | pRO1600/ColE1 | rhaT–rhaS–rhaR-PrhaB–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS81 | Sm/Sp | pRO1600/ColE1 | araE–araC-ParaB–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS66 | Sm/Sp | pRO1600/ColE1 | xylS-Pm–>mcherry | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS84 | Gm | pBBR1 | xylS-Pm–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS85 | Gm | pBBR1 | araC-ParaB–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS86 | Gm | pBBR1 | lacI-PT7–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS87 | Gm | pBBR1 | rhaS–rhaR-PrhaB–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS89 | Gm | pBBR1 | alkS-PalkB–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS90 | Gm | pBBR1 | nahR-Psal–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS91 | Gm | pBBR1 | lacI-PlacUV5–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS93 | Gm | pBBR1 | rhaT–rhaS–rhaR-PrhaB–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS94 | Gm | pBBR1 | araE–araC-ParaB–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS95 | Km | RK2 | xylS-Pm–>mcherry | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pPS131 | Gm | RSF1010 | rhaS–rhaR-PrhaB–>GFP(mut3) | Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pSIJ218 | Km | R6K | pEMG derived, T7 polymerase flanked by homologous regions for insertion after glmS gene |
Nielsen´s lab | Calero P. et al ACS Synthetic Biology. 2016 | |
pBSd141 | Ap | pRO1600/ColE1 | mRFP1 (const. Expressed); IIs-sites «destination» |
Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSd191 | Ap | pBR322/ROP | mRFP1 (const. Expressed); IIs-sites «destination» |
Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc241ble | Km+Pm* | pRO1600/ColE1 | MCS-default; PmR, IIs-sites «cargo» | Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc241cat | Km+Cm* | pRO1600/ColE1 | MCS-default; CmR, IIs-sites «cargo» | Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc241mls | Km+MLS* | pRO1600/ColE1 | MCS-default; MLSR, IIs-sites «cargo» | Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc241spec | Km+Sm/Sp* | pRO1600/ColE1 | MCS-default; SpR, IIs-sites «cargo» | Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc241tet-short | Km+Tc* | pRO1600/ColE1 | MCS-default; TcR, IIs-sites «cargo» | Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc241zeo | Km+Zc* | pRO1600/ColE1 | MCS-default; ZcR, IIs-sites «cargo» | Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc243ble | Km+Pm* | pRO1600/ColE1 | lacZα-pUC18; PmR, IIs-sites «cargo» |
Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc243cat | Km+Cm* | pRO1600/ColE1 | lacZα-pUC18; CmR, IIs-sites «cargo» |
Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc243mls | Km+MLS* | pRO1600/ColE1 | lacZα-pUC18; MLSR, IIs-sites «cargo» |
Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc243spec | Km+Sm/Sp* | pRO1600/ColE1 | lacZα-pUC18; SpR, IIs-sites «cargo» |
Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc243tet-short | Km+Tc* | pRO1600/ColE1 | lacZα-pUC18; TcR, IIs-sites «cargo» |
Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc243zeo | Km+Zc* | pRO1600/ColE1 | lacZα-pUC18; ZcR, IIs-sites «cargo» |
Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc291kan | Km+Km* | pBR322/ROP | MCS-default; KmR, IIs-sites «cargo» | Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc293kan | Km+Km* | pBR322/ROP | lacZα-pUC18; KmR, IIs-sites «cargo» |
Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc391kan | Cm+Km* | pBR322/ROP | MCS-default; CmR, IIs-sites «cargo» | Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc393kan | Cm+Km* | pBR322/ROP | lacZα-pUC18; CmR, IIs-sites «cargo» |
Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc241 | Km | pRO1600/ColE1 | MCS-default; IIs-sites «cargo» | Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc243 | Km | pRO1600/ColE1 | lacZα-pUC18; IIs-sites «cargo» |
Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc291 | Km | pBR322/ROP | MCS-default; IIs-sites «cargo» | Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc293 | Km | pBR322/ROP | lacZα-pUC18; IIs-sites «cargo» |
Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc391 | Cm | pBR322/ROP | MCS-default; IIs-sites «cargo» | Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pBSc393 | Cm | pBR322/ROP | lacZα-pUC18 | Mascher´s lab | Radeck J. et al. Scientific Reports. 2017 | |
pSEVA224-AEB | Km | RK2 | lacIq–Ptrc | Müller´s lab | Jahn M. et al. Microbial Cell Factories. 2016 | |
pSEVA234-AEB | Km | RK2 | lacIq–Ptrc | Müller´s lab | Jahn M. et al. Microbial Cell Factories. 2016 | |
pSEVA244-AEB | Km | RK2 | lacIq–Ptrc | Müller´s lab | Jahn M. et al. Microbial Cell Factories. 2016 | |
pSEVA254-AEB | Km | RK2 | lacIq–Ptrc | Müller´s lab | Jahn M. et al. Microbial Cell Factories. 2016 | |
pSEVA264-AEB | Km | RK2 | lacIq–Ptrc | Müller´s lab | Jahn M. et al. Microbial Cell Factories. 2016 | |
pSEVA274-AEB | Km | RK2 | lacIq–Ptrc | Müller´s lab | Jahn M. et al. Microbial Cell Factories. 2016 | |
pSEVA284-AEB | Km | RK2 | lacIq–Ptrc | Müller´s lab | Jahn M. et al. Microbial Cell Factories. 2016 | |
pABCa | Gm | pMlb / p15A | syn Ter1; Design type A | KY031724 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pABCb | Sm/Sp | p42b / pMB1 | syn Ter2; Design type A | KY031725 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pABCc | Km | p42d / pSC101* | syn Ter3; Design type A | KY031726 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pABCc-mob | Km | p42d / pSC101* | syn Ter3; mob; Design type B | KY031727 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pABC1 | Sm/Sp | p42b / pMB1 | syn Ter1; Design type A | KY031728 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pABC1-loxA | Sm/Sp | p42b / pMB1 | syn Ter1-loxL; mob-loxR; Design type C1 |
KY031729 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pABC1-loxB | Sm/Sp | p42b / pMB1 | syn Ter1-loxL; mob-loxR-rrnBT2; Design type C2 |
KY031730 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pABC1-sT2 | Sm/Sp | p42b / pMB1 | syn Ter2; Design type A | KY031731 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pABC1-sT3 | Sm/Sp | p42b / pMB1 | syn Ter3; Design type A | KY031732 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pABC2 | Gm | pMla / p15A | syn Ter2; Design type A | KY031733 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pABC3 | hygromycin | pTi / pSC101* | syn Ter3; Design type A | KY031734 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pABC4 | Sm/Sp | p42e / pMB1 | syn Ter1; Design type A | KY031735 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pABC5 | Sm/Sp | p42f1 / pMB1 | syn Ter2; Design type A | KY031736 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pABC6 | Sm/Sp | p42f2 / pMB1 | syn Ter2; Design type A | KY031737 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pLoriV-42b | Km | oriVp42b (R. etli) | KY031698 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pLoriV-42d | Km | oriVp42d (R. etli) | KY031699 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pLoriV-42e | Km | oriVp42e (R. etli) | KY031700 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pLoriV-42f1 | Km | oriVp42f – repA1B1C1 (R. etli) | KY031701 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pLoriV-42f2 | Km | oriVp42f – repA2B2C2 (R. etli) | KY031702 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pLoriV-Mla | Km | oriVpMla (M. loti) | KY031703 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pLoriV-Mlb | Km | oriVpMlb (M. loti) | KY031704 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pLoriV-Ti | Km | oriVpTi (A. tumefaciens) | KY031705 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pLsynTer-1 | Km | pMB1 | syn Ter1-MCS | KY031706 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pLsynTer-2 | Km | pMB1 | syn Ter2-MCS | KY031707 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pLsynTer-3 | Km | pMB1 | syn Ter3-MCS | KY031708 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pLsynTer-1-lox | Km | pMB1 | syn Ter1-MCS-loxL | KY031709 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pLsynTer-2-lox | Km | pMB1 | syn Ter2-MCS-loxL | KY031710 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pLsynTer-3-lox | Km | pMB1 | syn Ter3-MCS-loxL | KY031711 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pLoriT-1 | Km | pMB1 | mob | KY031712 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pLoriT-1-lox | Km | pMB1 | mob-loxR | KY031713 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pLoriT-1-loxT | Km | pMB1 | mob-loxR-rrnBT2 | KY031714 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
pLAR-Km | Km | pMB1 | KY031715 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pLAR-Spec | Sm/Sp, Km | pMB1 | KY031716 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pLAR-Tc | Tet, Km | pMB1 | KY031717 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pLAR-Hyg | Hygromycin, Km | pMB1 | KY031718 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pLAR-Tmp | Trimethoprim, Km | pMB1 | KY031719 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pLAR-Gm | Gm, Km | pMB1 | KY031720 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pK18mob2 | Km | pMB1 | KY031721 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pACYC184 | Tet | p15A | KY031722 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pXG-10 | Cm | pSC101* | KY031723 | Becker´s lab | Döhlemann J. et al. ACS Synthetic Biology. 2017 |
|
pRK99 | Km, Gm | R6K | MN626472 | Prieto´s lab | ||
pRK100 | Km | RK2 | MN626473 | Prieto´s lab | ||
pRK101 | Km | pRO1600/ColE1 | MN626474 | Prieto´s lab | ||
pRK102 | Gm | RK2 | MN626475 | Prieto´s lab | ||
pRK177 | Tet | pRO1600/ColE1 | MN626476 | Prieto´s lab | ||
pBAM1-GFP | Ap,Km | R6K | mini-Tn5 transposon | de Lorenzo´s lab | ||
pSEVAb127 | Ap | RK2 | Bba_J23105-GFP | MW476665.1 | Martins dos Santos´ lab | |
pSEVAb137 | Ap | pBBR1 | Bba_J23105-GFP | MW476666.1 | Martins dos Santos´ lab | |
pSEVAb147 | Ap | pRO1600/ColE1 | Bba_J23105-GFP | MW476667.1 | Martins dos Santos´ lab | |
pSEVAb157 | Ap | RSF1010 | Bba_J23105-GFP | MW476668.1 | Martins dos Santos´ lab | |
pSEVAb227 | Km | RK2 | Bba_J23105-GFP | MW476669.1 | Martins dos Santos´ lab | |
pSEVAb237 | Km | pBBR1 | Bba_J23105-GFP | MW476670.1 | Martins dos Santos´ lab | |
pSEVAb247 | Km | pRO1600/ColE1 | Bba_J23105-GFP | MW476671.1 | Martins dos Santos´ lab | |
pSEVAb257 | Km | RSF1010 | Bba_J23105-GFP | MW476672.1 | Martins dos Santos´ lab | |
pSEVAb427 | Sm/Sp | RK2 | Bba_J23105-GFP | MW476673.1 | Martins dos Santos´ lab | |
pSEVAb437 | Sm/Sp | pBBR1 | Bba_J23105-GFP | MW296039.1 | Martins dos Santos´ lab | |
pSEVAb447 | Sm/Sp | pRO1600/ColE1 | Bba_J23105-GFP | MW476674.1 | Martins dos Santos´ lab | |
pSEVAb457 | Sm/Sp | RSF1010 | Bba_J23105-GFP | MW476675.1 | Martins dos Santos´ lab | |
pSEVAb627 | Gm | RK2 | Bba_J23105-GFP | MW476676.1 | Martins dos Santos´ lab | |
pSEVAb637 | Gm | pBBR1 | Bba_J23105-GFP | MW476677.1 | Martins dos Santos´ lab | |
pSEVAb647 | Gm | pRO1600/ColE1 | Bba_J23105-GFP | MW476678.1 | Martins dos Santos´ lab | |
pSEVAb657 | Gm | RSF1010 | Bba_J23105-GFP | MW476679.1 | Martins dos Santos´ lab | |
pSEVA251-mVenus | Km | RSF1010 | mVenus | Luis Lopez Maury | ||
pSEVA251-turquoise | Km | RSF1010 | turquoise | Luis Lopez Maury | ||
pSEVA451-mVenus | Sm/Sp | RSF1010 | mVenus | Luis Lopez Maury | ||
pSEVA451-turquoise | Sm/Sp | RSF1010 | turquoise | Luis Lopez Maury | ||
pSEVA221::Amer-F1 | Km | RK2 | Amer-F1; NOT gate | OP271959 | de Lorenzo´s lab | |
pSEVA221::AmtR-A1 | Km | RK2 | AmtR-A1; NOT gate | OP254164 | de Lorenzo´s lab | |
pSEVA221::BetI-E1 | Km | RK2 | BetI-E1; NOT gate | OP254165 | de Lorenzo´s lab | |
pSEVA221::BM3R1-B1 | Km | RK2 | BM3R1-B1; NOT gate | OP254166 | de Lorenzo´s lab | |
pSEVA221::BM3R1-B2 | Km | RK2 | BM3R1-B2; NOT gate | OP254167 | de Lorenzo´s lab | |
pSEVA221::BM3R1-B3 | Km | RK2 | BM3R1-B3; NOT gate | OP254168 | de Lorenzo´s lab | |
pSEVA221::HIyIIR-H1 | Km | RK2 | HIyIIR-H1; NOT gate | OP254169 | de Lorenzo´s lab | |
pSEVA221::lcaRA-I1 | Km | RK2 | lcaRA-I1; NOT gate | OP254170 | de Lorenzo´s lab | |
pSEVA221::LitR-L1 | Km | RK2 | LitR-L1; NOT gate | OP254171 | de Lorenzo´s lab | |
pSEVA221::LmrA-N1 | Km | RK2 | LmrA-N1; NOT gate | OP254172 | de Lorenzo´s lab | |
pSEVA221::PhIF-P1 | Km | RK2 | PhIF-P1; NOT gate | OP254173 | de Lorenzo´s lab | |
pSEVA221::PhIF-P2 | Km | RK2 | PhIF-P2; NOT gate | OP254174 | de Lorenzo´s lab | |
pSEVA221::PhIF-P3 | Km | RK2 | PhIF-P3; NOT gate | OP254175 | de Lorenzo´s lab | |
pSEVA221::PsrA-R1 | Km | RK2 | PsrA-R1; NOT gate | OP254176 | de Lorenzo´s lab | |
pSEVA221::QacR-Q1 | Km | RK2 | QacR-Q1; NOT gate | OP254177 | de Lorenzo´s lab | |
pSEVA221::QacR-Q2 | Km | RK2 | QacR-Q2; NOT gate | OP254178 | de Lorenzo´s lab | |
pSEVA221::SrpR-S1 | Km | RK2 | SrpR-S1; NOT gate | OP254179 | de Lorenzo´s lab | |
pSEVA221::SrpR-S2 | Km | RK2 | SrpR-S2; NOT gate | OP254180 | de Lorenzo´s lab | |
pSEVA221::SrpR-S3 | Km | RK2 | SrpR-S3; NOT gate | OP254181 | de Lorenzo´s lab | |
pSEVA221::SrpR-S4 | Km | RK2 | SrpR-S4; NOT gate | OP254182 | de Lorenzo´s lab | |
pSEVA221::NOR-SrpR-S1 | Km | RK2 | NOR-SrpR-S1; NOR gate | OP254183 | de Lorenzo´s lab | |
pSEVA221::1201 | Km | RK2 | Empty plasmid for autofluorescence | de Lorenzo´s lab | ||
pSEVA221::1717 | Km | RK2 | RPU standard plasmid for standardization | de Lorenzo´s lab | ||
pSEVA221::1818 | Km | RK2 | pTac activity plasmid for promoter activity | de Lorenzo´s lab | ||
pSEVA221::1718 | Km | RK2 | Inducer Characterization – IPTG | de Lorenzo´s lab | ||
pSEVA221::1719 | Km | RK2 | Inducer Characterization – aTc | de Lorenzo´s lab | ||
pSEVA221::1720 | Km | RK2 | Inducer Characterization – arabinose | de Lorenzo´s lab | ||
pSEVA1MIN11 | Ap Min variant | R6K | MCS-default | pSEVA1MIN11.gb | Daniel Daley & Morten Norholm´ | Cumming AJ. et al. ACS Synthetic Biology. 2022 |
pSEVA1MIN21 | Ap Min variant | RK2 | MCS-default | pSEVA1MIN21.gb | Daniel Daley & Morten Norholm´ | Cumming AJ. et al. ACS Synthetic Biology. 2022 |
pSEVA1MIN31 | Ap Min variant | pBBR1 | MCS-default | pSEVA1MIN31.gb | Daniel Daley & Morten Norholm´ | Cumming AJ. et al. ACS Synthetic Biology. 2022 |
pSEVA1MIN41 | Ap Min variant | pRO1600/ColE1 | MCS-default | pSEVA1MIN41.gb | Daniel Daley & Morten Norholm´ | Cumming AJ. et al. ACS Synthetic Biology. 2022 |
pSEVA1MIN51 | Ap Min variant | RFS1010 | MCS-default | pSEVA1MIN51.gb | Daniel Daley & Morten Norholm´ | Cumming AJ. et al. ACS Synthetic Biology. 2022 |
pSEVA1MIN61 | Ap Min variant | p15A | MCS-default | pSEVA1MIN61.gb | Daniel Daley & Morten Norholm´ | Cumming AJ. et al. ACS Synthetic Biology. 2022 |
pSEVA1MIN81 | Ap Min variant | pUC | MCS-default | pSEVA1MIN81.gb | Daniel Daley & Morten Norholm´ | Cumming AJ. et al. ACS Synthetic Biology. 2022 |
pSEVA1MIN91 | Ap Min variant | pBR322/ROP | MCS-default | pSEVA1MIN91.gb | Daniel Daley & Morten Norholm´ | Cumming AJ. et al. ACS Synthetic Biology. 2022 |
pS2313B | Km | pBBR1 | PEM7 –> mBlue2 | pS2313B.gb | Pablo I. Nikel´s lab | |
pS2313O | Km | pBBR1 | PEM7 –> mOrange2 | pS2313O.gb | Pablo I. Nikel´s lab | |
pBLAM1-2 | Ap,Km | R6K | mariner mini-transposon | pBLAM1-2.gb | Goñi-Moreno´s lab | |
pBLAM1-4 | Ap, Sm/Sp | R6K | mariner mini-transposon | pBLAM1-4.gb | Goñi-Moreno´s lab | |
pBLAM1-6 | Ap, Gm | R6K | mariner mini-transposon | pBLAM1-6.gb | Goñi-Moreno´s lab | |
pSEVA421-Cas9tracrRNA | Sm/Sp | RK2 | Constitutive expresion of Cas9-tracrRNA | pSEVA421-Cas9-tracrRNA.gbk | de Lorenzo´s lab | Aparicio T. et al. Biotech. J, 2018 |
pSEVA658-ssr | Gm | RSF1010 | Inducible expression of Ssr | pSEVA658-ssr.gbk | de Lorenzo´s lab | Aparicio T. et al. Biotech. J, 2016 |
pSEVA6511-GIIi | Gm | RSF1010 | chnR-PchnB –> Ll.LtrB – Ll.LtrB group II intron | de Lorenzo´s lab | Velazquez E. et al. ACS Synth. Biol. 2021. | |
pS23Pm::GFP | Km | pBBR1 | Pm–> msfGFP | Pablo I. Nikel´s lab | Volke DC. et al. Microb. Biotech. 2020 | |
pS628SRED | Gm | RK2 | xylS-Pm–> I-sceI — P14g(BCD2) –> rfp | Pablo I. Nikel´s lab | Volke DC. et al. Metab. Eng. Commun. 2020. | |
pS228SRFP | Km | RK2 | xylS-Pm–> I-sceI — P14g(BCD2) –> rfp | Pablo I. Nikel´s lab | Volke DC. et al. Metab. Eng. Commun. 2020. | |
pQURE6・H | Gm | Conditionally replicating RK2 | xylS-Pm–> I-sceI — P14g –> rfp | Pablo I. Nikel´s lab | Volke DC. et al. Metab. Eng. Commun. 2020. | |
pTn7-xylS | Gm, Km | R6K | Pablo I. Nikel´s lab | Volke DC. et al. Microb. Biotech. 2020 | ||
pTn7-xylS-Nia | Gm, Km | R6K | Pablo I. Nikel´s lab | Volke DC. et al. Microb. Biotech. 2020 | ||
pSNW2 | Km | R6K | P14g(BCD2) –> msfGFP | Pablo I. Nikel´s lab | Volke DC. et al. Metab. Eng. Commun. 2020. | |
pSNW4 | Sm/Sp | R6K | P14g(BCD2) –> msfGFP | Pablo I. Nikel´s lab | Volke DC. et al. Metab. Eng. Commun. 2020. | |
pSNW5 | Tet | R6K | P14g(BCD2) –> msfGFP | Pablo I. Nikel´s lab | Volke DC. et al. Metab. Eng. Commun. 2020. | |
pSNW6 | Gm | R6K | P14g(BCD2) –> msfGFP | Pablo I. Nikel´s lab | Volke DC. et al. Metab. Eng. Commun. 2020. | |
pGNW2・LPG | Km | R6K | P14g(BCD2) –> msfGFP | Pablo I. Nikel´s lab | Wirth NT. et al. Microb. Biotech. 2020. | |
pS448・CsR | Sm/Sp | pRO1600/ColE1 | Pm–> cas9 — PEM7 –> sgRNA | Pablo I. Nikel´s lab | Wirth NT. et al. Microb. Biotech. 2020. |