LOCUS       pS2313-O                3855 bp ds-DNA     circular     10-APR-2019
DEFINITION  .
ACCESSION   
VERSION     
KEYWORDS    "Responsible person/group" "Usage" "Status" "Parent plasmids"      
            "Cloning sites" "Antibiotic resistance" "Origin of replication"
            "Promoters" "RBS" "Genes" "Tags" "Markers" "Spacers" "gRNAs"
            "Terminators" "DNA fragments" "Backbones" "Design entry"
            "LabCollector ID" "Comment" "Box" "Description" "Name"
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     misc_feature    complement(1663..1669)
                     /label="RBS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1677..2387
                     /label="mOrange2"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(533..1391)
                     /label="oriV"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     gene            complement(join(3725..3855,1..532))
                     /label="Rep"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        1521..1587
                     /label="promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      complement(1406..1510)
                     /label="T1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     gene            2624..3439
                     /label="Km"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      2394..2496
                     /label="T0"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(3471..3716)
                     /label="oriT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 GCAGGTCTGG TTCCATGCCC GGAATGCCGG GCTGGCTGGG CGGCTCCTCG CCGGGGCCGG
       61 TCGGTAGTTG CTGCTCGCCC GGATACAGGG TCGGGATGCG GCGCAGGTCG CCATGCCCCA
      121 ACAGCGATTC GTCCTGGTCG TCGTGATCAA CCACCACGGC GGCACTGAAC ACCGACAGGC
      181 GCAACTGGTC GCGGGGCTGG CCCCACGCCA CGCGGTCATT GACCACGTAG GCCGACACGG
      241 TGCCGGGGCC GTTGAGCTTC ACGACGGAGA TCCAGCGCTC GGCCACCAAG TCCTTGACTG
      301 CGTATTGGAC CGTCCGCAAA GAACGTCCGA TGAGCTTGGA AAGTGTCTTC TGGCTGACCA
      361 CCACGGCGTT CTGGTGGCCC ATCTGCGCCA CGAGGTGATG CAGCAGCATT GCCGCCGTGG
      421 GTTTCCTCGC AATAAGCCCG GCCCACGCCT CATGCGCTTT GCGTTCCGTT TGCACCCAGT
      481 GACCGGGCTT GTTCTTGGCT TGAATGCCGA TTTCTCTGGA CTGCGTGGCC ATGCTTATCT
      541 CCATGCGGTA GGGgTGCCGC ACGGTTGCGG CACCATGCGC AATCAGCTGC AACTTTTCGG
      601 CAGCGCGACA ACAATTATGC GTTGCGTAAA AGTGGCAGTC AATTACAGAT TTTCTTTAAC
      661 CTACGCAATG AGCTATTGCG GGGGGTGCCG CAATGAGCTG TTGCGTACCC CCCTTTTTTA
      721 AGTTGTTGAT TTTTAAGTCT TTCGCATTTC GCCCTATATC TAGTTCTTTG GTGCCCAAAG
      781 AAGGGCACCC CTGCGGGGTT CCCCCACGCC TTCGGCGCGG CTCCCCCTCC GGCAAAAAGT
      841 GGCCCCTCCG GGGCTTGTTG ATCGACTGCG CGGCCTTCGG CCTTGCCCAA GGTGGCGCTG
      901 CCCCCTTGGA ACCCCCGCAC TCGCCGCCGT GAGGCTCGGG GGGCAGGCGG GCGGGCTTCG
      961 CCcTTCGACT GCCCCCACTC GCATAGGCTT GGGTCGTTCC AGGCGCGTCA AGGCCAAGCC
     1021 GCTGCGCGGT CGCTGCGCGA GCCTTGACCC GCCTTCCACT TGGTGTCCAA CCGGCAAGCG
     1081 AAGCGCGCAG GCCGCAGGCC GGAGGCTTTT CCCCAGAGAA AATTAAAAAA ATTGATGGGG
     1141 CAAGGCCGCA GGCCGCGCAG TTGGAGCCGG TGGGTATGTG GTCGAAGGCT GGGTAGCCGG
     1201 TGGGCAATCC CTGTGGTCAA GCTCGTGGGC AGGCGCAGCC TGTCCATCAG CTTGTCCAGC
     1261 AGGGTTGTCC ACGGGCCGAG CGAAGCGAGC CAGCCGGTGG CCGCTCGCGG CCATCGTCCA
     1321 CATATCCACG GGCTGGCAAG GGAGCGCAgc gaccgcgcag ggcgaagccc ggagagcaag
     1381 cccgtagggg gGGCGCGCCC AGCTGTCTAG GGCGGCGGAT TTGTCCTACT CAGGAGAGCG
     1441 TTCACCGACA AACAACAGAT AAAACGAAAG GCCCAGTCTT TCGACTGAGC CTTTCGTTTT
     1501 ATTTGATGCC TTTAATtaaT TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA
     1561 TAATACGACA AGGTGAGGAA CTAAACCCCT AGGCCGCGGC CGCGCGAATT CGAGCTCGGT
     1621 ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTaggaggaa aaacatatgg
     1681 tgagcaaggg cgaggagaat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     1741 gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     1801 cctacgaggg ctttcagacc gctaagctga aggtgaccaa gggtggcccc ctgcccttcg
     1861 cctgggacat cctgtcccct catttcacct acggctccaa ggcctacgtg aagcaccccg
     1921 ccgacatccc cgactacttc aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     1981 tgaactacga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     2041 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtgatgc
     2101 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggtgccc
     2161 tgaagggcaa gatcaagatg aggctgaagc tgaaggacgg cggccactac acctccgagg
     2221 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtGgaca
     2281 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     2341 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaaACT AGTCTTGGAC
     2401 TCCTGTTGAT AGATCCAGTA ATGACCTCAG AACTCCATCT GGATTTGTTC AGAACGCTCG
     2461 GTTGCCGCCG GGCGTTTTTT ATTGGTGAGA ATCCAGGGGT CCCCAATAAT TACGATTTAA
     2521 ATTTGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAAATATAT CATCATGAAC
     2581 AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAGCG
     2641 TGAAACGAGC TGTAGCCGTC CGCGTCTGAA CAGCAACATG GATGCGGATC TGTATGGCTA
     2701 TAAATGGGCG CGTGATAACG TGGGTCAGAG CGGCGCGACC ATTTATCGTC TGTATGGCAA
     2761 ACCGGATGCG CCGGAACTGT TTCTGAAACA TGGCAAAGGC AGCGTGGCGA ACGATGTGAC
     2821 CGATGAAATG GTGCGTCTGA ACTGGCTGAC CGAATTTATG CCGCTGCCGA CCATTAAACA
     2881 TTTTATTCGC ACCCCGGATG ATGCGTGGCT GCTGACCACC GCGATTCCGG GCAAAACCGC
     2941 GTTTCAGGTG CTGGAAGAAT ATCCGGATAG CGGCGAAAAC ATTGTGGATG CGCTGGCCGT
     3001 GTTTCTGCGT CGTCTGCATA GCATTCCGGT GTGCAACTGC CCGTTTAACA GCGATCGTGT
     3061 GTTTCGTCTG GCCCAGGCGC AGAGCCGTAT GAACAACGGC CTGGTGGATG CGAGCGATTT
     3121 TGATGATGAA CGTAACGGCT GGCCGGTGGA ACAGGTGTGG AAAGAAATGC ATAAACTGCT
     3181 GCCGTTTAGC CCGGATAGCG TGGTGACCCA CGGCGATTTT AGCCTGGATA ACCTGATTTT
     3241 CGATGAAGGC AAACTGATTG GCTGCATTGA TGTGGGCCGT GTGGGCATTG CGGATCGTTA
     3301 TCAGGATCTG GCCATTCTGT GGAACTGCCT GGGCGAATTT AGCCCGAGCC TGCAAAAACG
     3361 TCTGTTTCAG AAATATGGCA TTGATAATCC GGATATGAAC AAACTGCAAT TTCATCTGAT
     3421 GCTGGATGAA TTTTTCTAAT AATTAATTGG ACcgcggtcc gcgcgttgtc CTTTTCCGCT
     3481 GCATAACCCT GCTTCGGGGT CATTATAGCG ATTTTTTCGG TATATCCATC CTTTTTCGCA
     3541 CGATATACAG GATTTTGCCA AAGGGTTCGT GTAGACTTTC CTTGGTGTAT CCAACGGCGT
     3601 CAGCCGGGCA GGATAGGTGA AGTAGGCCCA CCCGCGAGCG GGTGTTCCTT CTTCACTGTC
     3661 CCTTATTCGC ACCTGGCGGT GCTCAACGGG AATCCTGCTC TGCGAGGCTG GCCGTAGGCC
     3721 GGCCCTACCG GCGCGGCAGC GTTACCCGTG TCGGCGGCTC CAACGGCTCG CCATCGTCCA
     3781 GAAAACACGG CTCATCGGGC ATCGGCAGGC GCTGCTGCCC GCGCCGTTCC CATTCCTCCG
     3841 TTTCGGTCAA GGCTG
//