LOCUS       pS2313-B                3858 bp ds-DNA     circular     10-APR-2019
DEFINITION  .
ACCESSION   
VERSION     
KEYWORDS    "Responsible person/group" "Usage" "Status" "Parent plasmids"      
            "Cloning sites" "Antibiotic resistance" "Origin of replication"
            "Promoters" "RBS" "Genes" "Tags" "Markers" "Spacers" "gRNAs"
            "Terminators" "DNA fragments" "Backbones" "Design entry"
            "LabCollector ID" "Comment" "Box" "Description" "Name"
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     misc_feature    complement(1665..1671)
                     /label="RBS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     gene            2629..3444
                     /label="Km"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(535..1393)
                     /label="oriV"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     gene            complement(join(3730..3858,1..534))
                     /label="Rep"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(3476..3721)
                     /label="oriT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      complement(1408..1512)
                     /label="T1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      2399..2501
                     /label="T0"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        1523..1589
                     /label="promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1679..2389
                     /label="mBFP2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 TGGCAGGTCT GGTTCCATGC CCGGAATGCC GGGCTGGCTG GGCGGCTCCT CGCCGGGGCC
       61 GGTCGGTAGT TGCTGCTCGC CCGGATACAG GGTCGGGATG CGGCGCAGGT CGCCATGCCC
      121 CAACAGCGAT TCGTCCTGGT CGTCGTGATC AACCACCACG GCGGCACTGA ACACCGACAG
      181 GCGCAACTGG TCGCGGGGCT GGCCCCACGC CACGCGGTCA TTGACCACGT AGGCCGACAC
      241 GGTGCCGGGG CCGTTGAGCT TCACGACGGA GATCCAGCGC TCGGCCACCA AGTCCTTGAC
      301 TGCGTATTGG ACCGTCCGCA AAGAACGTCC GATGAGCTTG GAAAGTGTCT TCTGGCTGAC
      361 CACCACGGCG TTCTGGTGGC CCATCTGCGC CACGAGGTGA TGCAGCAGCA TTGCCGCCGT
      421 GGGTTTCCTC GCAATAAGCC CGGCCCACGC CTCATGCGCT TTGCGTTCCG TTTGCACCCA
      481 GTGACCGGGC TTGTTCTTGG CTTGAATGCC GATTTCTCTG GACTGCGTGG CCATGCTTAT
      541 CTCCATGCGG TAGGGgTGCC GCACGGTTGC GGCACCATGC GCAATCAGCT GCAACTTTTC
      601 GGCAGCGCGA CAACAATTAT GCGTTGCGTA AAAGTGGCAG TCAATTACAG ATTTTCTTTA
      661 ACCTACGCAA TGAGCTATTG CGGGGGGTGC CGCAATGAGC TGTTGCGTAC CCCCCTTTTT
      721 TAAGTTGTTG ATTTTTAAGT CTTTCGCATT TCGCCCTATA TCTAGTTCTT TGGTGCCCAA
      781 AGAAGGGCAC CCCTGCGGGG TTCCCCCACG CCTTCGGCGC GGCTCCCCCT CCGGCAAAAA
      841 GTGGCCCCTC CGGGGCTTGT TGATCGACTG CGCGGCCTTC GGCCTTGCCC AAGGTGGCGC
      901 TGCCCCCTTG GAACCCCCGC ACTCGCCGCC GTGAGGCTCG GGGGGCAGGC GGGCGGGCTT
      961 CGCCcTTCGA CTGCCCCCAC TCGCATAGGC TTGGGTCGTT CCAGGCGCGT CAAGGCCAAG
     1021 CCGCTGCGCG GTCGCTGCGC GAGCCTTGAC CCGCCTTCCA CTTGGTGTCC AACCGGCAAG
     1081 CGAAGCGCGC AGGCCGCAGG CCGGAGGCTT TTCCCCAGAG AAAATTAAAA AAATTGATGG
     1141 GGCAAGGCCG CAGGCCGCGC AGTTGGAGCC GGTGGGTATG TGGTCGAAGG CTGGGTAGCC
     1201 GGTGGGCAAT CCCTGTGGTC AAGCTCGTGG GCAGGCGCAG CCTGTCCATC AGCTTGTCCA
     1261 GCAGGGTTGT CCACGGGCCG AGCGAAGCGA GCCAGCCGGT GGCCGCTCGC GGCCATCGTC
     1321 CACATATCCA CGGGCTGGCA AGGGAGCGCA gcgaccgcgc agggcgaagc ccggagagca
     1381 agcccgtagg gggGGCGCGC CCAGCTGTCT AGGGCGGCGG ATTTGTCCTA CTCAGGAGAG
     1441 CGTTCACCGA CAAACAACAG ATAAAACGAA AGGCCCAGTC TTTCGACTGA GCCTTTCGTT
     1501 TTATTTGATG CCTTTAATta aTTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG
     1561 TATAATACGA CAAGGTGAGG AACTAAACCC CTAGGCCGCG GCCGCGCGAA TTCGAGCTCG
     1621 GTACCCGGGG ATCCTCTAGA GTCGACCTGC AGGCATGCAA GCTTaggagg aaaaacatat
     1681 ggtgtctaag ggcgaagagc tgattaagga gaacatgcac atgaagctgt acatggaggg
     1741 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
     1801 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
     1861 cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc
     1921 cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
     1981 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
     2041 caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac
     2101 actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag
     2161 aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac
     2221 atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta
     2281 cagactggaa agaatcaagg aggccaacaa cgagacGtac gtcgagcagc acgaggtggc
     2341 agtggccaga tactgcgacc tccctagcaa actggggcac aaActtaact aaACTAGTCT
     2401 TGGACTCCTG TTGATAGATC CAGTAATGAC CTCAGAACTC CATCTGGATT TGTTCAGAAC
     2461 GCTCGGTTGC CGCCGGGCGT TTTTTATTGG TGAGAATCCA GGGGTCCCCA ATAATTACGA
     2521 TTTAAATTTG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA
     2581 TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT
     2641 CAGCGTGAAA CGAGCTGTAG CCGTCCGCGT CTGAACAGCA ACATGGATGC GGATCTGTAT
     2701 GGCTATAAAT GGGCGCGTGA TAACGTGGGT CAGAGCGGCG CGACCATTTA TCGTCTGTAT
     2761 GGCAAACCGG ATGCGCCGGA ACTGTTTCTG AAACATGGCA AAGGCAGCGT GGCGAACGAT
     2821 GTGACCGATG AAATGGTGCG TCTGAACTGG CTGACCGAAT TTATGCCGCT GCCGACCATT
     2881 AAACATTTTA TTCGCACCCC GGATGATGCG TGGCTGCTGA CCACCGCGAT TCCGGGCAAA
     2941 ACCGCGTTTC AGGTGCTGGA AGAATATCCG GATAGCGGCG AAAACATTGT GGATGCGCTG
     3001 GCCGTGTTTC TGCGTCGTCT GCATAGCATT CCGGTGTGCA ACTGCCCGTT TAACAGCGAT
     3061 CGTGTGTTTC GTCTGGCCCA GGCGCAGAGC CGTATGAACA ACGGCCTGGT GGATGCGAGC
     3121 GATTTTGATG ATGAACGTAA CGGCTGGCCG GTGGAACAGG TGTGGAAAGA AATGCATAAA
     3181 CTGCTGCCGT TTAGCCCGGA TAGCGTGGTG ACCCACGGCG ATTTTAGCCT GGATAACCTG
     3241 ATTTTCGATG AAGGCAAACT GATTGGCTGC ATTGATGTGG GCCGTGTGGG CATTGCGGAT
     3301 CGTTATCAGG ATCTGGCCAT TCTGTGGAAC TGCCTGGGCG AATTTAGCCC GAGCCTGCAA
     3361 AAACGTCTGT TTCAGAAATA TGGCATTGAT AATCCGGATA TGAACAAACT GCAATTTCAT
     3421 CTGATGCTGG ATGAATTTTT CTAATAATTA ATTGGACcgc ggtccgcgcg ttgtcCTTTT
     3481 CCGCTGCATA ACCCTGCTTC GGGGTCATTA TAGCGATTTT TTCGGTATAT CCATCCTTTT
     3541 TCGCACGATA TACAGGATTT TGCCAAAGGG TTCGTGTAGA CTTTCCTTGG TGTATCCAAC
     3601 GGCGTCAGCC GGGCAGGATA GGTGAAGTAG GCCCACCCGC GAGCGGGTGT TCCTTCTTCA
     3661 CTGTCCCTTA TTCGCACCTG GCGGTGCTCA ACGGGAATCC TGCTCTGCGA GGCTGGCCGT
     3721 AGGCCGGCCC TACCGGCGCG GCAGCGTTAC CCGTGTCGGC GGCTCCAACG GCTCGCCATC
     3781 GTCCAGAAAA CACGGCTCAT CGGGCATCGG CAGGCGCTGC TGCCCGCGCC GTTCCCATTC
     3841 CTCCGTTTCG GTCAAGGC
//