LOCUS JX560337 3882 bp DNA circular SYN 30-SEP-2014 DEFINITION Cloning vector pSEVA421, complete sequence. ACCESSION JX560337 VERSION JX560337.2 GI:692118366 KEYWORDS . SOURCE Cloning vector pSEVA421 ORGANISM Cloning vector pSEVA421 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3882) AUTHORS Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B., Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D., Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de Lorenzo,V. TITLE The Standard European Vector Architecture (SEVA): a coherent platform for analysis and deployment of complex prokaryotic phenotypes JOURNAL Unpublished REFERENCE 2 (bases 1 to 3882) AUTHORS Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B., Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D., Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de Lorenzo,V. TITLE Direct Submission JOURNAL Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, Madrid 28049, Spain REFERENCE 3 (bases 1 to 3882) AUTHORS Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B., Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D., Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de Lorenzo,V. TITLE Direct Submission JOURNAL Submitted (30-SEP-2014) Systems Biology Program, Centro Nacional de Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, Madrid 28049, Spain REMARK Sequence update by submitter COMMENT On Sep 30, 2014 this sequence version replaced gi:410073704. ##Assembly-Data-START## Assembly Method :: ApE v. v2.0.40 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers primer_bind 9..32 /note="R24" primer_bind complement(121..144) /note="F24" terminator 151..253 /note="T0" gene 4995..5900 /gene="aadA" CDS 4995..5900 /dnas_title="spectinomycin adenyltransferase" /gene="aadA" /codon_start=1 /transl_table=11 /product="spectinomycin adenyltransferase" /protein_id="AFV59794.1" /db_xref="GI:410073705" /translation="MPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVI AEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRR ALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIF EPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWA GDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDR LASRADQLEEFVHYVKGEITKVVGK" misc_feature 5911..6156 /note="oriT" gene complement(6215..7363) /gene="trfA" CDS complement(6215..7363) /dnas_title="replication protein" /gene="trfA" /codon_start=1 /transl_table=11 /product="replication protein" /protein_id="AFV59795.1" /db_xref="GI:410073706" /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVG SIVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKK RKTAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALF TTRNKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPI TFTFYELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLD RGKKTSRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKL ETFRLMCGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" regulatory 8401..8505 /regulatory_class=terminator /note="T1" gene complement(261..4367) /note="cas9 (S. pyogenes)" misc_feature complement(4543..4748) /note="tracrRNA (S. pyogenes)" PCR_primer 209..225 /note="PS3" PCR_primer complement(8404..8421) /note="PS6" PCR_primer 5927..5944 /note="PS5" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc 121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg 181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt 241 ggtgagaatc caggggtccc TCAGTCACCT CCTAGCTGAC TCAAATCAAT GCGTGTTTCA 301 TAAAGACCAG TGATGGATTG ATGGATAAGA GTGGCATCTA AAACTTCTTT TGTAGACGTA 361 TATCGTTTAC GATCAATTGT TGTATCAAAA TATTTAAAAG CAGCGGGAGC TCCAAGATTC 421 GTCAACGTAA ATAAATGAAT AATATTTTCT GCTTGTTCAC GTATTGGTTT GTCTCTATGT 481 TTGTTATATG CACTAAGAAC TTTATCTAAA TTGGCATCTG CTAAAATAAC ACGCTTAGAA 541 AATTCACTGA TTTGCTCAAT AATCTCATCT AAATAATGCT TATGCTGCTC CACAAACAAT 601 TGTTTTTGTT CGTTATCTTC TGGACTACCC TTCAACTTTT CATAATGACT AGCTAAATAT 661 AAAAAATTCA CATATTTGCT TGGCAGAGCC AGCTCATTTC CTTTTTGTAA TTCTCCGGCA 721 CTAGCCAGCA TCCGTTTACG ACCGTTTTCT AACTCAAAAA GACTATATTT AGGTAGTTTA 781 ATGATTAAGT CTTTTTTAAC TTCCTTATAT CCTTTAGCTT CTAAAAAGTC AATCGGATTT 841 TTTTCAAAGG AACTTCTTTC CATAATTGTG ATCCCTAGTA ACTCTTTAAC GGATTTTAAC 901 TTCTTCGATT TCCCTTTTTC CACCTTAGCA ACCACTAGGA CTGAATAAGC TACCGTTGGA 961 CTATCAAAAC CACCATATTT TTTTGGATCC CAGTCTTTTT TACGAGCAAT AAGCTTGTCC 1021 GAATTTCTTT TTGGTAAAAT TGACTCCTTG GAGAATCCGC CTGTCTGTAC TTCTGTTTTC 1081 TTGACAATAT TGACTTGGGG CATGGACAAT ACTTTGCGCA CTGTGGCAAA ATCTCGCCCT 1141 TTATCCCAGA CAATTTCTCC AGTTTCCCCA TTAGTTTCGA TTAGAGGGCG TTTGCGAATC 1201 TCTCCATTTG CAAGTGTAAT TTCTGTTTTG AAGAAGTTCA TGATATTAGA GTAAAAGAAA 1261 TATTTTGCGG TTGCTTTGCC TATTTCTTGC TCAGACTTAG CAATCATTTT ACGAACATCA 1321 TAAACTTTAT AATCACCATA GACAAACTCC GATTCAAGTT TTGGATATTT CTTAATCAAA 1381 GCAGTTCCAA CGACGGCATT TAGATACGCA TCATGGGCAT GATGGTAATT GTTAATCTCA 1441 CGTACTTTAT AGAATTGGAA ATCTTTTCGG AAGTCAGAAA CTAATTTAGA TTTTAAGGTA 1501 ATCACTTTAA CCTCTCGAAT AAGTTTATCA TTTTCATCGT ATTTAGTATT CATGCGACTA 1561 TCCAAAATTT GTGCCACATG CTTAGTGATT TGGCGAGTTT CAACCAATTG GCGTTTGATA 1621 AAACCAGCTT TATCAAGTTC ACTCAAACCT CCACGTTCAG CTTTCGTTAA ATTATCAAAC 1681 TTACGTTGAG TGATTAACTT GGCGTTTAGA AGTTGTCTCC AATAGTTTTT CATCTTTTTG 1741 ACTACTTCTT CACTTGGAAC GTTATCCGAT TTACCACGAT TTTTATCAGA ACGCGTTAAG 1801 ACCTTATTGT CTATTGAATC GTCTTTAAGG AAACTTTGTG GAACAATGTG ATCGACATCA 1861 TAATCACTTA AACGATTAAT ATCTAATTCT TGGTCCACAT ACATGTCTCT TCCATTTTGG 1921 AGATAATAGA GATAGAGCTT TTCATTTTGC AATTGAGTAT TTTCAACAGG ATGCTCTTTA 1981 AGAATCTGAC TTCCTAATTC TTTGATACCT TCTTCGATTC GTTTCATACG CTCTCGCGAA 2041 TTTTTCTGGC CCTTTTGAGT TGTCTGATTT TCACGTGCCA TTTCAATAAC GATATTTTCT 2101 GGCTTATGCC GCCCCATTAC TTTGACCAAT TCATCAACAA CTTTTACAGT CTGTAAAATA 2161 CCTTTTTTAA TAGCAGGGCT ACCAGCTAAA TTTGCAATAT GTTCATGTAA ACTATCGCCT 2221 TGTCCAGACA CTTGTGCTTT TTGAATGTCT TCTTTAAATG TCAAACTATC ATCATGGATC 2281 AGCTGCATAA AATTGCGATT GGCAAAACCA TCTGATTTCA AAAAATCTAA TATTGTTTTG 2341 CCAGATTGCT TATCCCTAAT ACCATTAATC AATTTTCGAG ACAAACGTCC CCAACCAGTA 2401 TAACGGCGAC GTTTAAGCTG TTTCATCACC TTATCATCAA AGAGGTGAGC ATATGTTTTA 2461 AGTCTTTCCT CAATCATCTC CCTATCTTCA AATAAGGTCA ATGTTAAAAC AATATCCTCT 2521 AAGATATCTT CATTTTCTTC ATTATCCAAA AAATCTTTAT CTTTAATAAT TTTTAGCAAA 2581 TCATGGTAGG TACCTAATGA AGCATTAAAT CTATCTTCAA CTCCTGAAAT TTCAACACTA 2641 TCAAAACATT CTATTTTTTT GAAATAATCT TCTTTTAATT GCTTAACGGT TACTTTTCGA 2701 TTTGTTTTGA AGAGTAAATC AACAATGGCT TTCTTCTGTT CACCTGAAAG AAATGCTGGT 2761 TTTCGCATTC CTTCAGTAAC ATATTTGACC TTTGTCAATT CGTTATAAAC CGTAAAATAC 2821 TCATAAAGCA AACTATGTTT TGGTAGTACT TTTTCATTTG GAAGATTTTT ATCAAAGTTT 2881 GTCATGCGTT CAATAAATGA TTGAGCTGAA GCACCTTTAT CGACAACTTC TTCAAAATTC 2941 CATGGGGTAA TTGTTTCTTC AGACTTCCGA GTCATCCATG CAAAACGACT ATTGCCACGC 3001 GCCAATGGAC CAACATAATA AGGAATTCGA AAAGTCAAGA TTTTTTCAAT CTTCTCACGA 3061 TTGTCTTTTA AAAATGGATA AAAGTCTTCT TGTCTTCTCA AAATAGCATG CAGCTCACCC 3121 AAGTGAATTT GATGGGGAAT AGAGCCGTTG TCAAAGGTCC GTTGCTTGCG CAGCAAATCT 3181 TCACGATTTA GTTTCACCAA TAATTCCTCA GTACCATCCA TTTTTTCTAA AATTGGTTTG 3241 ATAAATTTAT AAAATTCTTC TTGGCTAGCT CCCCCATCAA TATAACCTGC ATATCCGTTT 3301 TTTGATTGAT CAAAAAAGAT TTCTTTATAC TTTTCTGGAA GTTGTTGTCG AACTAAAGCT 3361 TTTAAAAGAG TCAAGTCTTG ATGATGTTCA TCGTAGCGTT TAATCATTGA AGCTGATAGG 3421 GGAGCCTTAG TTATTTCAGT ATTTACTCTT AGGATATCTG AAAGTAAAAT AGCATCTGAT 3481 AAATTCTTAG CTGCCAAAAA CAAATCAGCA TATTGATCTC CAATTTGCGC CAATAAATTA 3541 TCTAAATCAT CATCGTAAGT ATCTTTTGAA AGCTGTAATT TAGCATCTTC TGCCAAATCA 3601 AAATTTGATT TAAAATTAGG GGTCAAACCC AATGACAAAG CAATGAGATT CCCAAATAAG 3661 CCATTTTTCT TCTCACCGGG GAGCTGAGCA ATGAGATTTT CTAATCGTCT TGATTTACTC 3721 AATCGTGCAG AAAGAATCGC TTTAGCATCT ACTCCACTTG CGTTAATAGG GTTTTCTTCA 3781 AATAATTGAT TGTAGGTTTG TACCAACTGG ATAAATAGTT TGTCCACATC ACTATTATCA 3841 GGATTTAAAT CTCCCTCAAT CAAAAAATGA CCACGAAACT TAATCATATG CGCTAAGGCC 3901 AAATAGATTA AGCGCAAATC CGCTTTATCA GTAGAATCTA CCAATTTTTT TCGCAGATGA 3961 TAGATAGTTG GATATTTCTC ATGATAAGCA ACTTCATCTA CTATATTTCC AAAAATAGGA 4021 TGACGTTCAT GCTTCTTGTC TTCTTCCACC AAAAAAGACT CTTCAAGTCG ATGAAAGAAA 4081 CTATCATCTA CTTTCGCCAT CTCATTTGAA AAAATCTCCT GTAGATAACA AATACGATTC 4141 TTCCGACGTG TATACCTTCT ACGAGCTGTC CGTTTGAGAC GAGTCGCTTC CGCTGTCTCT 4201 CCACTGTCAA ATAAAAGAGC CCCTATAAGA TTTTTTTTGA TACTGTGGCG GTCTGTATTT 4261 CCCAGAACCT TGAACTTTTT AGACGGAACC TTATATTCAT CAGTGATCAC CGCCCATCCG 4321 ACGCTATTTG TGCCGATATC TAAGCCTATT GAGTATTTCT TATCCATTTT TGCCTCCTAA 4381 AATAAAAAGT TTAAATTAAA TCCATAATGA GTTTGATGAT TTCAATAATA GTTTTAATGA 4441 CCTCCGAAAT TAGTTTAATA TGCTTTAATT TTTCTTTTTC AAAATATCTC TTCAAAAAAT 4501 ATTACCCAAT ACTTAATAAT AAATAGATTA TAACACAAAA TTCTTTTAAA AAGTAGTTTA 4561 TTTTGTTATC ATTCTATAGT ATTAAGTATT GTTTTATGGC TGATAAATTT CTTTGAATTT 4621 CTCCTTGATT ATTTGTTATA AAAGTTATAA AATAATCTTG TTGGAACCAT TCAAAACAGC 4681 ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG 4741 CTTTTTTTGA TACTTCTATT CTACTCTGAC TGCAAACCAA AAAAACAAGC GCTTTCAAAA 4801 CGCTTGTTTT ATCATTTTTA GGGAAATTAA TCTCTTAATC CTTTTATCAT TCTACATTTA 4861 GGCGCTGCCA TCTTGCTAAA CCTACTAAGC TCCACAGGAT GATatttaaa tgaaccttga 4921 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt 4981 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat 5041 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt 5101 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg 5161 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag 5221 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc 5281 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg 5341 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc 5401 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc 5461 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag 5521 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc 5581 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg 5641 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag 5701 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg 5761 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc 5821 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca 5881 ccaaggtagt cggcaaataa gacaattgtc cttttccgct gcataaccct gcttcggggt 5941 cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca 6001 aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga 6061 agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt 6121 gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc ggccgcgatg caggtggctg 6181 ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat 6241 cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga 6301 cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt 6361 ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg 6421 tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca 6481 gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca 6541 ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga 6601 agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc 6661 cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc 6721 cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg 6781 cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct 6841 tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg 6901 ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa 6961 gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcttcgc 7021 tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc 7081 gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac 7141 gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct 7201 cgatcttggc cgtagcttgc tggactatcg agccgacgga ctggaaggtt tcgcggggcg 7261 cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga 7321 tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga 7381 ttgccccgga attaattccc cggatcgatc cgtcgatctt gatcccctgc gccatcagat 7441 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg 7501 cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg 7561 ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca 7621 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta 7681 cgtggctgcc atttttgggg tgaggccgtt cgcggccgag gggcgcagcc cctgggggga 7741 tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg 7801 tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata aatattggtt 7861 taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa 7921 tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc cctcatctgt 7981 cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc 8041 tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac 8101 tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac gtcgccggcc 8161 gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt 8221 caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc 8281 ggcggcccta catggctctg ctgtagtgag tgggttgcgc tccggcagcg gtcctgatcc 8341 cccgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacggcg cgcccagctg 8401 tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac 8461 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcct //