LOCUS       Exported                2631 bp ds-DNA     circular SYN 16-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 16, 2022 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          280..1318
                     /organism="Cloning vector pSEVA111"
                     /mol_type="other DNA"
                     /db_xref="taxon:1246683"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    complement(33..120)
                     /label=MCS
     primer_bind     121..144
                     /label=F24
                     /note="F24"
     terminator      complement(151..253)
                     /label=T0 terminator
     CDS             414..1274
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(1347..1551)
                     /label=OriT
     rep_origin      complement(1613..2480)
                     /direction=LEFT
                     /label=pUC Ori
     terminator      complement(2526..2630)
                     /label=T1 terminator
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg
      301 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaagggga tgtatgagta
      421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
      661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
      961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1261 ttaagcattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga
     1321 cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta
     1381 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct
     1441 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg
     1501 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg
     1561 cgaggctggc cgtaggccgg ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1621 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1681 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1741 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1801 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1861 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1921 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1981 agatacctac agcgtgagct ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2041 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2101 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2161 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2221 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2281 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2341 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     2401 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     2461 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc aggcgcgccc
     2521 agctgtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     2581 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc t
//
