LOCUS JX560350 5153 bp DNA circular SYN 31-OCT-2012 DEFINITION Cloning vector pSEVA651, complete sequence. ACCESSION JX560350 VERSION JX560350.1 GI:410073745 KEYWORDS . SOURCE Cloning vector pSEVA651 ORGANISM Cloning vector pSEVA651 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5153) AUTHORS Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B., Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D., Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de Lorenzo,V. TITLE The Standard European Vector Architecture (SEVA): a coherent platform for analysis and deployment of complex prokaryotic phenotypes JOURNAL Unpublished REFERENCE 2 (bases 1 to 5153) AUTHORS Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B., Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D., Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de Lorenzo,V. TITLE Direct Submission JOURNAL Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, Madrid 28049, Spain COMMENT ##Assembly-Data-START## Assembly Method :: ApE v. v2.0.40 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..7871 /organism="Cloning vector pSEVA651" /mol_type="other DNA" /db_xref="taxon:1246772" terminator 2869..2971 /note="T0" gene 3215..3748 /dnas_title="acc3" /gene="acc3" CDS 3215..3748 /dnas_title="gentamicin-3-acetyltransferase" /gene="acc3" /codon_start=1 /transl_table=11 /product="gentamicin-3-acetyltransferase" /protein_id="AFV59822.1" /db_xref="GI:410073746" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAAL DLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQP RSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKL GIREEVMHFDIDPSTAT" misc_feature 3834..4069 /note="oriT" gene complement(4078..5028) /dnas_title="repC" /gene="repC" CDS complement(4078..5028) /dnas_title="replication protein" /gene="repC" /codon_start=1 /transl_table=11 /product="replication protein" /protein_id="AFV59825.1" /db_xref="GI:410073749" /translation="MAHRSLIGGSGGMTAGCSSPPCWRGSARARGCPVVKPKNKHSLS HVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDYGDGKRIEFSGPEPLGADDLRILQG LVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAVTAECHVVKGSYRALAKEIGAEVD SGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSEYASDEADGRLYVALNPLIAQAV MGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGKTGKASIDTLCGYVWPSEASGS TMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKAAG" gene complement(4916..5755) /dnas_title="repA" /gene="repA" CDS complement(4916..5755) /dnas_title="replication protein" /gene="repA" /codon_start=1 /transl_table=11 /product="replication protein" /protein_id="AFV59824.1" /db_xref="GI:410073748" /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKS MLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAV ADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVI GRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSA EAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPR GEA" gene complement(6266..7237) /dnas_title="repB" /gene="repB" CDS complement(6266..7237) /dnas_title="replication protein" /gene="repB" /codon_start=1 /transl_table=11 /product="replication protein" /protein_id="AFV59823.1" /db_xref="GI:410073747" /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEV LQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSP KNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRA GYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRR TALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKP GHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM" promoter complement(7262..7291) /note="PlacUV5" terminator 7766..7870 /note="T1" gene complement(19..984) /label=xylS promoter 1928..1963 /label=Pm primer_bind 1980..2054 /dnas_title="tggagtcatgaccatgcctaggccgcggccgcgcgaattcAGAAG GAGAatataccATGAGCCAAGTAGCCAGGGTC" /pair="Gibson-PP-Beta-F" /primer="tggagtcatgaccatgcctaggccgcggccgcgcgaattcAGAAGGAGA atataccATGAGCCAAGTAGCCAGGGTC" /current=1 PCR_primer 1869..1890 /dnas_title="470" /pair="GGTTTGATAGGGATAAGTCCAG" /primer="470" /current=1 CDS 2036..2794 /dnas_title="PP-beta" /vntifkey=4 /label=PP-beta primer_bind 2839..2862 /note="F24" primer_bind complement(2773..2834) /dnas_title="ccgcaagcttgcatgcctgcaggtcgactctagaggatccTTACT CCTGCGGCCGGCTGGCC" /pair="Gibson-PP-Beta-Rv" /primer="ccgcaagcttgcatgcctgcaggtcgactctagaggatccTTACTCCTG CGGCCGGCTGGCC" /current=1 ORIGIN 1 ttaattaaac gttcgtaatc aagccacttc ctttttgcat tgacgcaggg tgtcggaagg 61 caactcgccg aacgcgctcc tatagttttc agcgaagcgt cccaaatgta agaagccgta 121 gtctagggct atctcagtta tactacgcac attggcactg ggatcgttca agcaggcgcg 181 gatgctttcg agcttgcggt tgcggatgta gttcttcggc gtggtgccgg cgtgcttctc 241 gaacaaattg tagagcgagc gtggactcat catcgccagc tccgctaacc gctcaaggct 301 gatattccgt ttgagattct cctcaatgaa ttgaacgact cgctcgaaag acgggttacc 361 tttgctgaaa atttcacggc tgacattgct gcccagcatt tcgagcagct tggaagcgat 421 gatccccgca tagtgctctt ggacccgagg catcgacttt gtatgttccg cttcgtcaca 481 aactaacccg agtagattga taaagccatc gagttgctgg agattgtgtc gcgcggcgaa 541 acggataccc tccctcggct tgtgccaatt gttgtcactg cacgcccgat caaggaccac 601 tgagggcaat ttaacgataa atttctcgca atcttctgaa taggtcaggt cggcttggtc 661 atccggattg agcagcaata gttcgcccgg cgcaaaatag tgctcctggc catggccacg 721 ccacaggcaa tggcctttga gtattatttg cagatgataa caggtctcta atccaggcga 781 gattaccctc acgctaccgc cgtagctgat tcgacacaga tcgaggcatc cgaagattct 841 gtggtgcagc ctgcctgccg ggcgcccgcc cttgggcagg cgaatagagt gcgtaccgac 901 atactggtta acataatcgg agactgcata gggctcggcg tggacgaaga tctgactttt 961 ctcgttcaat aagcaaaaat ccatagttca cggttctctt attttaatgt gggctgcttg 1021 gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct cgacgtgatg cgtatacggg 1081 ttacccccat tgccacgttg cgccatcctt tttgcaatca gtgaccactt ttccaagcaa 1141 aaataacgcc aagcagaacg aagacgttct ttttaagaag cgagaacacc agaagttcgt 1201 gctgtcgggg catggggcga cgaattggcg gataaagggg atctgctgga tattacggcc 1261 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt 1321 gcccgcctga tgaatgctca tccgtaatta cgtatggcaa tgaaagacgg tgagctggtg 1381 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca 1441 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat 1501 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt 1561 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg 1621 gacaacttct tcgcccccgt tttcaccatg catgggaatt agcttgatct gaccaacgac 1681 cggtagcgga gctatccaac ggcggtatac caggaaaaca cacagcaggt acatcagaac 1741 agtaccatga ctgaagaaca aatagttttt tcctgatcca taaagcagaa cggcctgctc 1801 catgacaaat ctggctcccc aactaatgcc ccatgcagcc agcataacca gcataaagtg 1861 cagtgtccgg tttgataggg ataagtccag ccttgcaaga agcggataca ggagtgcaaa 1921 aaatggctat ctctagtaag gcctacccct taggctttat gcaacagaaa caataataat 1981 ggagtcatga ccatgcctag gccgcggccg cgcgaattca gaAGGAGAat ataccatgag 2041 ccaagtagcc agggtcgaaa cccattccca gccacaggct gtagccgccg agtcggcgac 2101 catcctgcag atcatccagc aagtcgcaat gtcccccaat gcagacatcg acaagatgga 2161 gcgattgatg gcgatgcacc gccagcacca agcacaacag gcgcagcaag cgttcgatgc 2221 cgcgctagcc gccatgcagg aagagcttcc ggttatccgt gagcgcggcg caatcaagga 2281 caagtacaag aacgtccaat ctacctacgc cctatgggag gacatcaacg aagagttgaa 2341 gcccatcctg gcgaagcacg gctttgctct caccttccga attccacgca ccgacaaagg 2401 catcgaggtt gagggcgtgc ttagtcatcg agacggccac agggaaacca catcaatcct 2461 cctgccagcc gatgctaccg gcagcaagaa tgccgtccag gccgttgcca gttctgtcag 2521 ctacgggaag cgctacacgg ccggcgctct tctgaacttc accactactg gcgaagacga 2581 tgacggccaa ggagccgtac cgatgcaagt gcctgatgag ccggtcatca caccgcgcca 2641 agccgcccaa ctcgatgggc tgctgaaaaa atgcagccaa gtcctggtcg acaacttcac 2701 cgccaagtac ggctgcgctg ccaacgtcta caaatccgag ttcgacgttg tgctcgctcg 2761 cctcaccaag tcggccagcc ggccgcagga gtaaggatcc tctagagtcg acctgcaggc 2821 atgcaagctt gcggccgcgt cgtgactggg aaaaccctgg cgactagtct tggactcctg 2881 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc 2941 cgccgggcgt tttttattgg tgagaatcca ggggtcccca ataattacga tttaaatttg 3001 acataagcct gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg 3061 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt 3121 tatgactgtt tttttgtaca gcctatgcct cgggcatcca agcagcaagc gcgttacgcc 3181 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcagca acgatgttac 3241 gcagcagggc agtcgcccta aaacaaagtt aggtggctca agtatgggca tcattcgcac 3301 atgtaggctc ggccctgacc aagtcaaatc catgcgggct gctcttgatc ttttcggtcg 3361 tgagttcgga gacgtagcca cctactccca acatcagccg gactccgatt acctcgggaa 3421 cttgctccgt agtaagacat tcatcgcgct tgctgccttc gaccaagaag cggttgttgg 3481 cgctctcgcg gcttacgttc tgcccaagtt tgagcagccg cgtagtgaga tctatatcta 3541 tgatctcgca gtctccggag agcaccggag gcagggcatt gccaccgcgc tcatcaatct 3601 cctcaagcat gaggccaacg cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg 3661 tgacgatccc gcagtggctc tctatacaaa gttgggcata cgggaagaag tgatgcactt 3721 tgatatcgac ccaagtaccg ccacctaaca attcgttcaa gccgagatcg gcttcccggc 3781 cgcggagttg ttcggtaaat tggacaacgg tccgcgcgtt gtccttttcc gctgcataac 3841 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata 3901 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg 3961 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt 4021 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag gccggcctca 4081 gcctgccgcc ttgggccggg tgatgtcgta cttgcccgcc gcgaactcgg ttaccgtcca 4141 gcccagcgcg accagctccg gcaacgcctc gcgcacccgc tggcggcgct tgcgcatggt 4201 cgaaccactg gcctctgacg gccagacata gccgcacaag gtatctatgg aagccttgcc 4261 ggttttgccg gggtcgatcc agccacacag ccgctggtgc agcaggcggg cggtttcgct 4321 gtccagcgcc cgcacctcgt ccatgctgat gcgcacatgc tggccgccac ccatgacggc 4381 ctgcgcgatc aaggggttca gggccacgta caggcgcccg tccgcctcgt cgctggcgta 4441 ctccgacagc agccgaaacc cctgccgctt gcggccattc tgggcgatga tggatacctt 4501 ccaaaggcgc tcgatgcagt cctgtatgtg cttgagcgcc ccaccactat cgacctctgc 4561 cccgatttcc tttgccagcg cccgatagct acctttgacc acatggcatt cagcggtgac 4621 ggcctcccac ttgggttcca ggaacagccg gagctgccgt ccgccttcgg tcttgggttc 4681 cgggccaagc actaggccat taggcccagc catggccacc agcccttgca ggatgcgcag 4741 atcatcagcg cccagcggct ccgggccgct gaactcgatc cgcttgccgt cgccgtagtc 4801 atacgtcacg tccagcttgc tgcgcttgcg ctcgccccgc ttgagggcac ggaacaggcc 4861 gggggccaga cagtgcgccg ggtcgtgccg gacgtggctg aggctgtgct tgttcttagg 4921 cttcaccacg gggcaccccc ttgctcttgc gctgcctctc cagcacggcg ggcttgagca 4981 ccccgccgtc atgccgcctg aaccaccgat cagcgaacgg tgcgccatag ttggccttgc 5041 tcacaccgaa gcggacgaag aaccggcgct ggtcgtcgtc cacaccccat tcctcggcct 5101 cggcgctggt catgctcgac aggtaggact gccagcggat gttatcgacc agtaccgagc 5161 tgccccggct ggcctgctgc tggtcgcctg cgcccatcat ggccgcgccc ttgctggcat 5221 ggtgcaggaa cacgatagag cacccggtat cggcggcgat ggcctccatg cgaccgatga 5281 cctgggccat ggggccgctg gcgttttctt cctcgatgtg gaaccggcgc agcgtgtcca 5341 gcaccatcag gcggcggccc tcggcggcgc gcttgaggcc gtcgaaccac tccggggcca 5401 tgatgttggg caggctgccg atcagcggct ggatcagcag gccgtcagcc acggcttgcc 5461 gttcctcggc gctgaggtgc gccccaaggg cgtgcaggcg gtgatgaatg gcggtgggcg 5521 ggtcttcggc gggcaggtag atcaccgggc cggtgggcag ttcgcccacc tccagcagat 5581 ccggcccgcc tgcaatctgt gcggccagtt gcagggccag catggattta ccggcaccac 5641 cgggcgacac cagcgccccg accgtaccgg ccaccatgtt gggcaaaacg tagtccagcg 5701 gtggcggcgc tgctgcgaac gcctccagaa tattgatagg cttatgggta gccattgatt 5761 gcctcctttg caggcagttg gtggttaggc gctggcgggg tcactacccc cgccctgcgc 5821 cgctctgagt tcttccaggc actcgcgcag cgcctcgtat tcgtcgtcgg tcagccagaa 5881 cttgcgctga cgcatccctt tggccttcat gcgctcggca tatcgcgctt ggcgtacagc 5941 gtcagggctg gccagcaggt cgccggtctg cttgtccttt tggtctttca tatcagtcac 6001 cgagaaactt gccggggccg aaaggcttgt cttcgcggaa caaggacaag gtgcagccgt 6061 caaggttaag gctggccata tcagcgactg aaaagcggcc agcctcggcc ttgtttgacg 6121 tataaccaaa gccaccgggc aaccaatagc ccttgtcact tttgatcagg tagaccgacc 6181 ctgaagcgct tttttcgtat tccataaaac ccccttctgt gcgtgagtac tcatagtata 6241 acaggcgtga gtaccaacgc aagcactaca tgctgaaatc tggcccgccc ctgtccatgc 6301 ctcgctggcg gggtgccggt gcccgtgcca gctcggcccg cgcaagctgg acgctgggca 6361 gacccatgac cttgctgacg gtgcgctcga tgtaatccgc ttcgtggccg ggcttgcgct 6421 ctgccagcgc tgggctggcc tcggccatgg ccttgccgat ttcctcggca ctgcggcccc 6481 ggctggccag cttctgcgcg gcgataaagt cgcacttgct gaggtcatca ccgaagcgct 6541 tgaccagccc ggccatctcg ctgcggtact cgtccagcgc cgtgcgccgg tggcggctaa 6601 gctgccgctc gggcagttcg aggctggcca gcctgcgggc cttctcctgc tgccgctggg 6661 cctgctcgat ctgctggcca gcctgctgca ccagcgccgg gccagcggtg gcggtcttgc 6721 ccttggattc acgcagcagc acccacggct gataaccggc gcgggtggtg tgcttgtcct 6781 tgcggttggt gaagcccgcc aagcggccat agtggcggct gtcggcgctg gccgggtcgg 6841 cgtcgtactc gctggccagc gtccgggcaa tctgcccccg aagttcaccg cctgcggcgt 6901 cggccacctt gacccatgcc tgatagttct tcgggctggt ttccactacc agggcaggct 6961 cccggccctc ggctttcatg tcatccaggt caaactcgct gaggtcgtcc accagcacca 7021 gaccatgccg ctcctgctcg gcgggcctga tatacacgtc attgccctgg gcattcatcc 7081 gcttgagcca tggcgtgttc tggagcactt cggcggctga ccattcccgg ttcatcatct 7141 ggccggtggt ggcgtccctg acgccgatat cgaagcgctc acagcccatg gccttgagct 7201 gtcggcctat ggcctgcaaa gtcctgtcgt tcttcatcgg gccaccaagc gattcccaca 7261 cattatacga gccggaagca taaagtgtaa agcctagatc cgaaggatga gccgggctga 7321 atgatcgacc gagacaggcc ctgcggggct gcacacgcgc ccccaccctt cgggtagggg 7381 gaaaggccgc taaagcggct aaaagcgctc cagcgtattt ctgcggggtt tggtgtgggg 7441 tttagcgggc tttgcccgcc tttccccctg ccgcgcagcg gtggggcggt gtgtagccta 7501 gcgcagcgaa tagaccagct atccggcctc tggccgggca tattgggcaa gggcagcagc 7561 gccccacaag ggcgctgata accgcgccta gtggattatt cttagataat catggatgga 7621 tttttccaac accccgccag cccccgcccc tgctgggttt gcaggtttgg gggcgtgaca 7681 gttattgcag gggttcgtga cagttattgc aggggggcgt gacagttatt gcaggggttc 7741 gtgacagtta gggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg 7801 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt 7861 atttgatgcc t //