LOCUS Exported 2434 bp ds-DNA circular SYN 16-AUG-2022 DEFINITION Cloning vector pSEVA111, complete sequence. ACCESSION JX560321 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2434) AUTHORS Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez G, Kim J, Nikel PI, Platero R, de Lorenzo V. TITLE The Standard European Vector Architecture (SEVA): a coherent platform for analysis and deployment of complex prokaryotic phenotypes JOURNAL Unpublished REFERENCE 2 (bases 1 to 2434) AUTHORS Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez G, Kim J, Nikel PI, Platero R, de Lorenzo V. TITLE Direct Submission JOURNAL Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, Madrid 28049, Spain REFERENCE 3 (bases 1 to 2434) AUTHORS Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez G, Kim J, Nikel PI, Platero R, de Lorenzo V. TITLE Direct Submission JOURNAL Submitted (25-SEP-2014) Systems Biology Program, Centro Nacional de Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, Madrid 28049, Spain REFERENCE 4 (bases 1 to 2434) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 16, 2022 from SnapGene 4.2.11 http://www.snapgene.com COMMENT On Sep 25, 2014 this sequence version replaced JX560321.1. ##Assembly-Data-START## Assembly Method :: ApE v. v2.0.40 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..2434 /organism="Cloning vector pSEVA111" /mol_type="other DNA" /db_xref="taxon:1246683" source 280..1318 /organism="Cloning vector pSEVA111" /mol_type="other DNA" /db_xref="taxon:1246683" source 1583..2314 /label=/dnas_title=pSEVA261 /note="/dnas_title=pSEVA261" primer_bind 9..32 /label=R24 /note="R24" primer_bind complement(121..144) /label=F24 /note="F24" regulatory 151..253 /regulatory_class="terminator" /note="T0" CDS 414..1274 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 1329..1574 /label=oriT /note="oriT" misc_feature 1583..2314 /label=p15A ori /note="p15A ori" regulatory 2329..2433 /regulatory_class="terminator" /note="T1" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc 121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg 181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt 241 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg 301 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc 361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaagggga tgtatgagta 421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg 661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag 961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 1021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 1081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 1141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 1201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 1261 ttaagcattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga 1321 cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta 1381 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct 1441 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg 1501 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg 1561 cgaggctggc cgtaggccgg ccctagaaat attttatctg attaataaga tgatcttctt 1621 gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg 1681 gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag 1741 gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga 1801 ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc 1861 gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt 1921 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga 1981 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg 2041 agagcgcacg agggagccgc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 2101 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg 2161 gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc 2221 aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt 2281 agcgagtcag tgagcgagga agcggaatat atccggcgcg cccagctgtc tagggcggcg 2341 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt 2401 ctttcgactg agcctttcgt tttatttgat gcct //