LOCUS Exported 3924 bp ds-DNA circular SYN 16-AUG-2022 DEFINITION Cloning vector pSEVA121, complete sequence. ACCESSION JX560322 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3924) AUTHORS Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez G, Kim J, Nikel PI, Platero R, de Lorenzo V. TITLE The Standard European Vector Architecture (SEVA): a coherent platform for analysis and deployment of complex prokaryotic phenotypes JOURNAL Unpublished REFERENCE 2 (bases 1 to 3924) AUTHORS Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez G, Kim J, Nikel PI, Platero R, de Lorenzo V. TITLE Direct Submission JOURNAL Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, Madrid 28049, Spain REFERENCE 3 (bases 1 to 3924) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 16, 2022 from SnapGene 4.2.11 http://www.snapgene.com COMMENT ##Assembly-Data-START## Assembly Method :: ApE v. v2.0.40 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3924 /organism="Cloning vector pSEVA121" /mol_type="other DNA" /db_xref="taxon:1246686" source 280..1318 /organism="Cloning vector pSEVA111" /mol_type="other DNA" /db_xref="taxon:1246683" primer_bind 9..32 /label=R24 /note="R24" primer_bind complement(121..144) /label=F24 /note="F24" regulatory 151..253 /regulatory_class="terminator" /note="T0" CDS 414..1274 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 1329..1574 /label=oriT /note="oriT" gene complement(1633..2781) /gene="trfA" /label=trfA CDS complement(1633..2781) /codon_start=1 /gene="trfA" /product="replication protein" /label=trfA /protein_id="AFV59762.1" /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" regulatory 3819..3923 /regulatory_class="terminator" /note="T1" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc 121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg 181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt 241 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg 301 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc 361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaagggga tgtatgagta 421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg 661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag 961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 1021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 1081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 1141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 1201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 1261 ttaagcattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga 1321 cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta 1381 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct 1441 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg 1501 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg 1561 cgaggctggc cgtaggccgg ccgcgatgca ggtggctgct gaacccccag ccggaactga 1621 ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac 1681 caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac 1741 gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc 1801 ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt 1861 ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat 1921 caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag 1981 cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag 2041 gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc 2101 ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta 2161 ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta 2221 ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg 2281 gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg 2341 catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg 2401 cttcgtgtgt ttcagcaacg cggcctgctt ggcttcgctg acctgttttg ccaggtcctc 2461 gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt 2521 cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc 2581 gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg 2641 gactatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc 2701 gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt 2761 ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccggaat taattccccg 2821 gatcgatccg tcgatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc 2881 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt 2941 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct 3001 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc 3061 atccggggtc agcaccgttt ctgcggactg gctttctacg tggctgccat ttttggggtg 3121 aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc 3181 cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc 3241 gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg 3301 ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga 3361 cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt 3421 caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc 3481 acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc 3541 gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc 3601 tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc 3661 agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccctaca tggctctgct 3721 gtagtgagtg ggttgcgctc cggcagcggt cctgatcccc cgcagaaaaa aaggatctca 3781 agaagatcct ttgatctttt ctacggcgcg cccagctgtc tagggcggcg gatttgtcct 3841 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg 3901 agcctttcgt tttatttgat gcct //