LOCUS Exported 3224 bp ds-DNA circular SYN 16-AUG-2022 DEFINITION Cloning vector pSEVA131, complete sequence. ACCESSION JX560323 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3224) AUTHORS Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez G, Kim J, Nikel PI, Platero R, de Lorenzo V. TITLE The Standard European Vector Architecture (SEVA): a coherent platform for analysis and deployment of complex prokaryotic phenotypes JOURNAL Unpublished REFERENCE 2 (bases 1 to 3224) AUTHORS Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez G, Kim J, Nikel PI, Platero R, de Lorenzo V. TITLE Direct Submission JOURNAL Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, Madrid 28049, Spain REFERENCE 3 (bases 1 to 3224) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 16, 2022 from SnapGene 4.2.11 http://www.snapgene.com COMMENT ##Assembly-Data-START## Assembly Method :: ApE v. v2.0.40 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3224 /organism="Cloning vector pSEVA131" /mol_type="other DNA" /db_xref="taxon:1246688" source 280..1318 /organism="Cloning vector pSEVA111" /mol_type="other DNA" /db_xref="taxon:1246683" primer_bind 9..32 /label=R24 /note="R24" primer_bind complement(121..144) /label=F24 /note="F24" regulatory 151..253 /regulatory_class="terminator" /note="T0" CDS 414..1274 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 1329..1574 /label=oriT /note="oriT" gene complement(1583..2245) /gene="rep" /label=rep CDS complement(1583..2245) /codon_start=1 /gene="rep" /product="replication protein" /label=rep /protein_id="AFV59764.1" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" regulatory 3119..3223 /regulatory_class="terminator" /note="T1" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc 121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg 181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt 241 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg 301 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc 361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaagggga tgtatgagta 421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg 661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag 961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 1021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 1081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 1141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 1201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 1261 ttaagcattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga 1321 cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta 1381 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct 1441 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg 1501 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg 1561 cgaggctggc cgtaggccgg ccctaccggc gcggcagcgt tacccgtgtc ggcggctcca 1621 acggctcgcc atcgtccaga aaacacggct catcgggcat cggcaggcgc tgctgcccgc 1681 gccgttccca ttcctccgtt tcggtcaagg ctggcaggtc tggttccatg cccggaatgc 1741 cgggctggct gggcggctcc tcgccggggc cggtcggtag ttgctgctcg cccggataca 1801 gggtcgggat gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg tcgtcgtgat 1861 caaccaccac ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc tggccccacg 1921 ccacgcggtc attgaccacg taggccgaca cggtgccggg gccgttgagc ttcacgacgg 1981 agatccagcg ctcggccacc aagtccttga ctgcgtattg gaccgtccgc aaagaacgtc 2041 cgatgagctt ggaaagtgtc ttctggctga ccaccacggc gttctggtgg cccatctgcg 2101 ccacgaggtg atgcagcagc attgccgccg tgggtttcct cgcaataagc ccggcccacg 2161 cctcatgcgc tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg gcttgaatgc 2221 cgatttctct ggactgcgtg gccatgctta tctccatgcg gtaggggtgc cgcacggttg 2281 cggcaccatg cgcaatcagc tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt 2341 aaaagtggca gtcaattaca gattttcttt aacctacgca atgagctatt gcggggggtg 2401 ccgcaatgag ctgttgcgta cccccctttt ttaagttgtt gatttttaag tctttcgcat 2461 ttcgccctat atctagttct ttggtgccca aagaagggca cccctgcggg gttcccccac 2521 gccttcggcg cggctccccc tccggcaaaa agtggcccct ccggggcttg ttgatcgact 2581 gcgcggcctt cggccttgcc caaggtggcg ctgccccctt ggaacccccg cactcgccgc 2641 cgtgaggctc ggggggcagg cgggcgggct tcgcccttcg actgccccca ctcgcatagg 2701 cttgggtcgt tccaggcgcg tcaaggccaa gccgctgcgc ggtcgctgcg cgagccttga 2761 cccgccttcc acttggtgtc caaccggcaa gcgaagcgcg caggccgcag gccggaggct 2821 tttccccaga gaaaattaaa aaaattgatg gggcaaggcc gcaggccgcg cagttggagc 2881 cggtgggtat gtggtcgaag gctgggtagc cggtgggcaa tccctgtggt caagctcgtg 2941 ggcaggcgca gcctgtccat cagcttgtcc agcagggttg tccacgggcc gagcgaagcg 3001 agccagccgg tggccgctcg cggccatcgt ccacatatcc acgggctggc aagggagcgc 3061 agcgaccgcg cagggcgaag cccggagagc aagcccgtag ggggggcgcg cccagctgtc 3121 tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga 3181 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcct //