LOCUS       Exported                2094 bp ds-DNA     circular SYN 16-AUG-2022
DEFINITION  Cloning vector pSEVA111, complete sequence.
ACCESSION   JX560321
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 2094)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2014) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   4  (bases 1 to 2094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 16, 2022 from SnapGene 4.2.11
            http://www.snapgene.com
COMMENT     On Sep 25, 2014 this sequence version replaced JX560321.1.
            ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Cloning vector pSEVA111"
                     /mol_type="other DNA"
                     /db_xref="taxon:1246683"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     regulatory      151..253
                     /regulatory_class="terminator"
                     /note="T0"
     CDS             414..1274
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1329..1574
                     /label=oriT
                     /note="oriT"
     rep_origin      1583..1974
                     /label=R6K
                     /note="R6K"
     regulatory      1989..2093
                     /regulatory_class="terminator"
                     /note="T1"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg
      301 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaagggga tgtatgagta
      421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
      661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
      961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1261 ttaagcattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga
     1321 cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta
     1381 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct
     1441 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg
     1501 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg
     1561 cgaggctggc cgtaggccgg ccgatctgaa gatcagcagt tcaacctgtt gatagtacgt
     1621 actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag ctctcatgtt
     1681 taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt actaagctct
     1741 catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa tcagcaactt
     1801 aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag gcttttaaag
     1861 cctttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg agaagccctt
     1921 agagcctctc aaagcaattt tgagtgacac aggaacactt aacggctgac atggggcgcg
     1981 cccagctgtc tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     2041 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcct
//