LOCUS Exported 5376 bp ds-DNA circular SYN 16-AUG-2022 DEFINITION Cloning vector pSEVA151, complete sequence. ACCESSION JX560325 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5376) AUTHORS Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez G, Kim J, Nikel PI, Platero R, de Lorenzo V. TITLE The Standard European Vector Architecture (SEVA): a coherent platform for analysis and deployment of complex prokaryotic phenotypes JOURNAL Unpublished REFERENCE 2 (bases 1 to 5376) AUTHORS Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez G, Kim J, Nikel PI, Platero R, de Lorenzo V. TITLE Direct Submission JOURNAL Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, Madrid 28049, Spain REFERENCE 3 (bases 1 to 5376) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 16, 2022 from SnapGene 4.2.11 http://www.snapgene.com COMMENT ##Assembly-Data-START## Assembly Method :: ApE v. v2.0.40 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5376 /organism="Cloning vector pSEVA151" /mol_type="other DNA" /db_xref="taxon:1246694" source 280..1318 /organism="Cloning vector pSEVA111" /mol_type="other DNA" /db_xref="taxon:1246683" primer_bind 9..32 /label=R24 /note="R24" primer_bind complement(121..144) /label=F24 /note="F24" regulatory 151..253 /regulatory_class="terminator" /note="T0" CDS 414..1274 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 1329..1574 /label=oriT /note="oriT" gene complement(1583..2533) /gene="repC" /label=repC CDS complement(1583..2533) /codon_start=1 /gene="repC" /product="replication protein" /label=repC /protein_id="AFV59770.1" /translation="MAHRSLIGGSGGMTAGCSSPPCWRGSARARGCPVVKPKNKHSLSH VRHDPAHCLAPGLFRALKRGERKRSKLDVTYDYGDGKRIEFSGPEPLGADDLRILQGLV AMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAVTAECHVVKGSYRALAKEIGAEVDSGG ALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSEYASDEADGRLYVALNPLIAQAVMGGG QHVRISMDEVRALDSETARLLHQRLCGWIDPGKTGKASIDTLCGYVWPSEASGSTMRKR RQRVREALPELVALGWTVTEFAAGKYDITRPKAAG" gene complement(2421..3260) /gene="repA" /label=repA CDS complement(2421..3260) /codon_start=1 /gene="repA" /product="replication protein" /label=repA /protein_id="AFV59769.1" /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA" gene complement(3771..4742) /gene="repB" /label=repB CDS complement(3771..4742) /codon_start=1 /gene="repB" /product="replication protein" /label=repB /protein_id="AFV59768.1" /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM" regulatory complement(4767..4796) /regulatory_class="promoter" /note="PlacUV5" regulatory 5271..5375 /regulatory_class="terminator" /note="T1" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc 121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg 181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt 241 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg 301 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc 361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaagggga tgtatgagta 421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg 661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag 961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 1021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 1081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 1141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 1201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 1261 ttaagcattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga 1321 cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta 1381 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct 1441 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg 1501 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg 1561 cgaggctggc cgtaggccgg cctcagcctg ccgccttggg ccgggtgatg tcgtacttgc 1621 ccgccgcgaa ctcggttacc gtccagccca gcgcgaccag ctccggcaac gcctcgcgca 1681 cccgctggcg gcgcttgcgc atggtcgaac cactggcctc tgacggccag acatagccgc 1741 acaaggtatc tatggaagcc ttgccggttt tgccggggtc gatccagcca cacagccgct 1801 ggtgcagcag gcgggcggtt tcgctgtcca gcgcccgcac ctcgtccatg ctgatgcgca 1861 catgctggcc gccacccatg acggcctgcg cgatcaaggg gttcagggcc acgtacaggc 1921 gcccgtccgc ctcgtcgctg gcgtactccg acagcagccg aaacccctgc cgcttgcggc 1981 cattctgggc gatgatggat accttccaaa ggcgctcgat gcagtcctgt atgtgcttga 2041 gcgccccacc actatcgacc tctgccccga tttcctttgc cagcgcccga tagctacctt 2101 tgaccacatg gcattcagcg gtgacggcct cccacttggg ttccaggaac agccggagct 2161 gccgtccgcc ttcggtcttg ggttccgggc caagcactag gccattaggc ccagccatgg 2221 ccaccagccc ttgcaggatg cgcagatcat cagcgcccag cggctccggg ccgctgaact 2281 cgatccgctt gccgtcgccg tagtcatacg tcacgtccag cttgctgcgc ttgcgctcgc 2341 cccgcttgag ggcacggaac aggccggggg ccagacagtg cgccgggtcg tgccggacgt 2401 ggctgaggct gtgcttgttc ttaggcttca ccacggggca cccccttgct cttgcgctgc 2461 ctctccagca cggcgggctt gagcaccccg ccgtcatgcc gcctgaacca ccgatcagcg 2521 aacggtgcgc catagttggc cttgctcaca ccgaagcgga cgaagaaccg gcgctggtcg 2581 tcgtccacac cccattcctc ggcctcggcg ctggtcatgc tcgacaggta ggactgccag 2641 cggatgttat cgaccagtac cgagctgccc cggctggcct gctgctggtc gcctgcgccc 2701 atcatggccg cgcccttgct ggcatggtgc aggaacacga tagagcaccc ggtatcggcg 2761 gcgatggcct ccatgcgacc gatgacctgg gccatggggc cgctggcgtt ttcttcctcg 2821 atgtggaacc ggcgcagcgt gtccagcacc atcaggcggc ggccctcggc ggcgcgcttg 2881 aggccgtcga accactccgg ggccatgatg ttgggcaggc tgccgatcag cggctggatc 2941 agcaggccgt cagccacggc ttgccgttcc tcggcgctga ggtgcgcccc aagggcgtgc 3001 aggcggtgat gaatggcggt gggcgggtct tcggcgggca ggtagatcac cgggccggtg 3061 ggcagttcgc ccacctccag cagatccggc ccgcctgcaa tctgtgcggc cagttgcagg 3121 gccagcatgg atttaccggc accaccgggc gacaccagcg ccccgaccgt accggccacc 3181 atgttgggca aaacgtagtc cagcggtggc ggcgctgctg cgaacgcctc cagaatattg 3241 ataggcttat gggtagccat tgattgcctc ctttgcaggc agttggtggt taggcgctgg 3301 cggggtcact acccccgccc tgcgccgctc tgagttcttc caggcactcg cgcagcgcct 3361 cgtattcgtc gtcggtcagc cagaacttgc gctgacgcat ccctttggcc ttcatgcgct 3421 cggcatatcg cgcttggcgt acagcgtcag ggctggccag caggtcgccg gtctgcttgt 3481 ccttttggtc tttcatatca gtcaccgaga aacttgccgg ggccgaaagg cttgtcttcg 3541 cggaacaagg acaaggtgca gccgtcaagg ttaaggctgg ccatatcagc gactgaaaag 3601 cggccagcct cggccttgtt tgacgtataa ccaaagccac cgggcaacca atagcccttg 3661 tcacttttga tcaggtagac cgaccctgaa gcgctttttt cgtattccat aaaaccccct 3721 tctgtgcgtg agtactcata gtataacagg cgtgagtacc aacgcaagca ctacatgctg 3781 aaatctggcc cgcccctgtc catgcctcgc tggcggggtg ccggtgcccg tgccagctcg 3841 gcccgcgcaa gctggacgct gggcagaccc atgaccttgc tgacggtgcg ctcgatgtaa 3901 tccgcttcgt ggccgggctt gcgctctgcc agcgctgggc tggcctcggc catggccttg 3961 ccgatttcct cggcactgcg gccccggctg gccagcttct gcgcggcgat aaagtcgcac 4021 ttgctgaggt catcaccgaa gcgcttgacc agcccggcca tctcgctgcg gtactcgtcc 4081 agcgccgtgc gccggtggcg gctaagctgc cgctcgggca gttcgaggct ggccagcctg 4141 cgggccttct cctgctgccg ctgggcctgc tcgatctgct ggccagcctg ctgcaccagc 4201 gccgggccag cggtggcggt cttgcccttg gattcacgca gcagcaccca cggctgataa 4261 ccggcgcggg tggtgtgctt gtccttgcgg ttggtgaagc ccgccaagcg gccatagtgg 4321 cggctgtcgg cgctggccgg gtcggcgtcg tactcgctgg ccagcgtccg ggcaatctgc 4381 ccccgaagtt caccgcctgc ggcgtcggcc accttgaccc atgcctgata gttcttcggg 4441 ctggtttcca ctaccagggc aggctcccgg ccctcggctt tcatgtcatc caggtcaaac 4501 tcgctgaggt cgtccaccag caccagacca tgccgctcct gctcggcggg cctgatatac 4561 acgtcattgc cctgggcatt catccgcttg agccatggcg tgttctggag cacttcggcg 4621 gctgaccatt cccggttcat catctggccg gtggtggcgt ccctgacgcc gatatcgaag 4681 cgctcacagc ccatggcctt gagctgtcgg cctatggcct gcaaagtcct gtcgttcttc 4741 atcgggccac caagcgattc ccacacatta tacgagccgg aagcataaag tgtaaagcct 4801 agatccgaag gatgagccgg gctgaatgat cgaccgagac aggccctgcg gggctgcaca 4861 cgcgccccca cccttcgggt agggggaaag gccgctaaag cggctaaaag cgctccagcg 4921 tatttctgcg gggtttggtg tggggtttag cgggctttgc ccgcctttcc ccctgccgcg 4981 cagcggtggg gcggtgtgta gcctagcgca gcgaatagac cagctatccg gcctctggcc 5041 gggcatattg ggcaagggca gcagcgcccc acaagggcgc tgataaccgc gcctagtgga 5101 ttattcttag ataatcatgg atggattttt ccaacacccc gccagccccc gcccctgctg 5161 ggtttgcagg tttgggggcg tgacagttat tgcaggggtt cgtgacagtt attgcagggg 5221 ggcgtgacag ttattgcagg ggttcgtgac agttagggcg cgcccagctg tctagggcgg 5281 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca 5341 gtctttcgac tgagcctttc gttttatttg atgcct //