LOCUS Exported 3671 bp ds-DNA circular SYN 16-AUG-2022 DEFINITION Cloning vector pSEVA141, complete sequence. ACCESSION JX560324 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3671) AUTHORS Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez G, Kim J, Nikel PI, Platero R, de Lorenzo V. TITLE The Standard European Vector Architecture (SEVA): a coherent platform for analysis and deployment of complex prokaryotic phenotypes JOURNAL Unpublished REFERENCE 2 (bases 1 to 3671) AUTHORS Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez G, Kim J, Nikel PI, Platero R, de Lorenzo V. TITLE Direct Submission JOURNAL Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, Madrid 28049, Spain REFERENCE 3 (bases 1 to 3671) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 16, 2022 from SnapGene 4.2.11 http://www.snapgene.com COMMENT ##Assembly-Data-START## Assembly Method :: ApE v. v2.0.40 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3671 /organism="Cloning vector pSEVA141" /mol_type="other DNA" /db_xref="taxon:1246691" source 280..1318 /organism="Cloning vector pSEVA111" /mol_type="other DNA" /db_xref="taxon:1246683" primer_bind 9..32 /label=R24 /note="R24" primer_bind complement(121..144) /label=F24 /note="F24" regulatory 151..253 /regulatory_class="terminator" /note="T0" CDS 414..1274 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 1329..1574 /label=oriT /note="oriT" gene complement(2308..3150) /gene="rep" /label=rep CDS complement(2308..3150) /codon_start=1 /gene="rep" /product="replication protein" /label=rep /protein_id="AFV59766.1" /translation="MSNVASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVT DEVMYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGW VQTIIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWD SIGQREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKV THLLFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA" regulatory 3566..3670 /regulatory_class="terminator" /note="T1" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc 121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg 181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt 241 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg 301 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc 361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaagggga tgtatgagta 421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg 661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag 961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 1021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 1081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 1141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 1201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 1261 ttaagcattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga 1321 cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta 1381 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct 1441 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg 1501 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg 1561 cgaggctggc cgtaggccgg ccgataatct catgaccaaa atcccttaac gtgagttttc 1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 1801 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 2101 gcatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 2161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 2221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg ccgtgaaagg 2281 caggccggtc cgtggtggcc acggcctcta ggccagatcc agcggcatct gggttagtcg 2341 agcgcgggcc gcttcccatg tctcaccagg gcgagcctgt ttcgcgatct cagcatctga 2401 aatcttcccg gccttgcgct tcgctggggc cttacccacc gccttggcgg gcttcttcgg 2461 tccaaaactg aacaacagat gtgtgacctt gcgcccggtc tttcgctgcg cccactccac 2521 ctgtagcggg ctgtgctcgt tgatctgcgt cacggctgga tcaagcactc gcaacttgaa 2581 gtccttgatc gagggatacc ggccttccag ttgaaaccac tttcgcagct ggtcaatttc 2641 tatttcgcgc tggccgatgc tgtcccattg catgagcagc tcgtaaagcc tgatcgcgtg 2701 ggtgctgtcc atcttggcca cgtcagccaa ggcgtatttg gtgaactgtt tggtgagttc 2761 cgtcaggtac ggcagcatgt ctttggtgaa cctgagttct acacggccct caccctcccg 2821 gtagatgatt gtttgcaccc agccggtaat catcacactc ggtcttttcc ccttgccatt 2881 gggctcttgg gttaaccgga cttcccgccg tttcaggcgc agggccgctt ctttgagctg 2941 gttgtaggaa gattcgatag ggacacccgc catcgtcgct atgtcctccg ccgtcactga 3001 atacatcact tcatcggtga caggctcgct cctcttcacc tggctaatac aggccagaac 3061 gatccgctgt tcctgaacac tgaggcgata cgcggcctcg accagggcat tgcttttgta 3121 aaccattggg ggtgaggcca cgttcgacat tccttgtgta taaggggaca ctgtatctgc 3181 gtcccacaat acaacaaatc cgtcccttta caacaacaaa tccgtccctt cttaacaaca 3241 aatccgtccc ttaatggcaa caaatccgtc cctttttaaa ctctacaggc cacggattac 3301 gtggcctgta gacgtcctaa aaggtttaaa agggaaaagg aagaaaaggg tggaaacgca 3361 aaaaacgcac cactacgtgg ccccgttggg gccgcatttg tgcccctgaa ggggcggggg 3421 aggcgtctgg gcaatccccg ttttaccagt cccctatcgc cgcctgagag ggcgcaggaa 3481 gcgagtaatc agggtatcga ggcggattca cccttggcgt ccaaccagcg gcaccagcgg 3541 cgcctgagag gggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg 3601 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt 3661 atttgatgcc t //