LOCUS       pPS39        5705 bp    DNA     circular SYN 28-JAN-2019
DEFINITION  Cloning vector pPS39, complete sequence.
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Cloning vector pSEVA441"
                     /mol_type="other DNA"
                     /db_xref="taxon:1246745"
                     /vntifkey="98"
     primer_bind     9..32
                     /vntifkey="28"
                     /label=Primer_Bind_1
                     /note="F24"
     CDS             complement(45..983)
                     /cds_type=ORF
                     /vntifkey="4"
                     /label=rhaR
     CDS             complement(967..1803)
                     /vntifkey="4"
                     /label=rhaS
     primer_bind     complement(2197..2220)
                     /vntifkey="28"
                     /label=Primer_Bind_2
                     /note="R24"
     terminator      2227..2329
                     /vntifkey="43"
                     /label=Terminator_1
                     /note="T0"
     CDS             2447..3352
                     /gene="aadA"
                     /codon_start=1
                     /transl_table=11
                     /product="spectinomycin adenyltransferase"
                     /protein_id="AFV59798.1"
                     /db_xref="GI:410073711"
                     /vntifkey="4"
                     /label=aadA
     gene            2447..3352
                     /gene="aadA"
                     /vntifkey="60"
     misc_feature    3373..3608
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="oriT"
     CDS             complement(4342..5184)
                     /gene="rep"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="AFV59799.1"
                     /db_xref="GI:410073712"
                     /vntifkey="4"
                     /label=rep
     gene            complement(4342..5184)
                     /gene="rep"
                     /vntifkey="60"
     terminator      5600..5704
                     /vntifkey="43"
                     /label=Terminator_2
                     /note="T1"
BASE COUNT     1362 a      1524 c      1489 g      1330 t 
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgatc gcgtttaatc tttctgcgaa 
       61 ttgagatgac gccactggct gggcgtcatc ccggtttccc gggtaaacac caccgaaaaa 
      121 tagttactat cttcaaagcc acattcggtc gaaatatcac tgattaacag gcggctatgc 
      181 tggagaagat attgcgcatg acacactctg acctgccgca gatattgatt gatggtcatt 
      241 ccagtctgct ggcgaaattg ctgacgcaaa acgcgctcac tgcacgatgc ctcatcacaa 
      301 aatttatcca gcgcaaaggg acttttcagg ctagccgcca gccgggtaat cagcttatcc 
      361 agcaacgttt cgctggatgt tggcggcaac gaatcactgg tgtaacgatg gcgattcagc 
      421 aacatcacca actgcccgaa cagcaactca cccatttcgt tagcaaacgg cacatgctga 
      481 ctactttcat gctcaagctg accgataatc tgccgcgcct gcgccatccc aacgctacct 
      541 aagcgccagt gtggttgccc tgcgctggcg ctaaatcccg gaatcgcccc ctgccagtca 
      601 agattcagct tcagccgctc tgggcaataa ataatattct gcaaaaccag atcgttaacg 
      661 gaagcgtagg agtgtttatc gtcagcatga atgtaaaaga gatcgccacg ggtaatgcga 
      721 taagggcgat cgttgagtac atgcagacca ttaccgcgcc agacaatcac cagctcacaa 
      781 aaatcatgtg tatgttcggc aaagacatct tgcggataac ggtcagccac agcgactgcc 
      841 tgctggtcgc tggcaaaaaa atcatctttg agaagtttta actgatgcac caccgtggct 
      901 acctcggcca gagaacgaag ttgattattc gcaatatggc gtacaaatac gttgagaaga 
      961 ttcgcgttat tgcagaaaac catcccgtcc ctgacgaata tcacgcggtg accagttaaa 
     1021 ctctcggcga aaaagcgtcg aaaagtggtt actgtcgctg aatccacagc gataggcgat 
     1081 gtcagtaacg ctggcctcac tgtggcgtag cagatgtcgg gctttcatca gtcgcaggcg 
     1141 attcaggtaa cgctgaggcg tcagtcccgt ttgctgctta agctgccgat gtagcgtacg 
     1201 cagtgaaaga gaaaattgat ccgccacggc atcccaattc acctcatcgg caaaatggtc 
     1261 ctccagccag gccagaagca agttgagacg tgatgcgctg ttttccaggt tctcctgcaa 
     1321 actgctttta cgcagcaaga gcagtaattg cataaacaag atctcgcgac tggcggtcga 
     1381 gggtaaatca ttttcccctt cctgctgttc catctgtgca accagctgtc gcacctgctg 
     1441 caatacgctg tggttaacgc gccagtgaga cggatactgc ccatccagct cttgtggcag 
     1501 caactgattc agcccggcga gaaactgaaa tcgatccggc gagcgataca gcacattggt 
     1561 cagacacaga ttatcggtat gttcatacag atgccgatca tgatcgcgta cgaaacagac 
     1621 cgtgccaccg gtgatggtat agggctgccc attaaacaca tgaatacccg tgccatgttc 
     1681 gacaatcaca atttcatgaa aatcatgatg atgttcagga aaatccgcct gcgggagccg 
     1741 gggttctatc gccacggaca cgttaccaga cggaaaaaaa tccacactat gtaatacggt 
     1801 catactggcc tcctgatgtc gtcaacacgg cgaaatagta atcacgaggt caggttctta 
     1861 ccttaaattt tcgacggaaa accacgtaaa aaacgtcgat ttttcaagat acagcgtgaa 
     1921 ttttcaggaa atgcggtgag catcacatca ccacaattca gcaaattgtg aacatcatca 
     1981 cgttcatctt tccctggttg ccaatggccc attttcctgt cagtaacgag aaggtcgcga 
     2041 attcaggcgc tttttagact ggtcgtaatg aaattcgctg aggcgatcgc cctaggccgc 
     2101 ggccgcgcga attcgagctc ggtacccggg gatcctctag agtcgagcgc atacctgcag 
     2161 gcatgcaagc ttgcggccgc gcgatcgcac ctcagcgtcg tgactgggaa aaccctggcg 
     2221 actagtcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg 
     2281 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccagg ggtccccaat 
     2341 aattacgatt tacgtattta aatgaacctt gaccgaacgc agcggtggta acggcgcagt 
     2401 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc 
     2461 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt 
     2521 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc 
     2581 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac 
     2641 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga 
     2701 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat 
     2761 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga 
     2821 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact 
     2881 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat 
     2941 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg 
     3001 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa 
     3061 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt 
     3121 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt 
     3181 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc 
     3241 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga 
     3301 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aagacccttg 
     3361 tccttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca 
     3421 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt 
     3481 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc 
     3541 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc 
     3601 tggccgtagg ccggccgata atctcatgac caaaatccct taacgtgagt tttcgttcca 
     3661 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 
     3721 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 
     3781 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 
     3841 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 
     3901 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 
     3961 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 
     4021 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 
     4081 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggcatcc 
     4141 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 
     4201 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 
     4261 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggccgtga aaggcaggcc 
     4321 ggtccgtggt ggccacggcc tctaggccag atccagcggc atctgggtta gtcgagcgcg 
     4381 ggccgcttcc catgtctcac cagggcgagc ctgtttcgcg atctcagcat ctgaaatctt 
     4441 cccggccttg cgcttcgctg gggccttacc caccgccttg gcgggcttct tcggtccaaa 
     4501 actgaacaac agatgtgtga ccttgcgccc ggtctttcgc tgcgcccact ccacctgtag 
     4561 cgggctgtgc tcgttgatct gcgtcacggc tggatcaagc actcgcaact tgaagtcctt 
     4621 gatcgaggga taccggcctt ccagttgaaa ccactttcgc agctggtcaa tttctatttc 
     4681 gcgctggccg atgctgtccc attgcatgag cagctcgtaa agcctgatcg cgtgggtgct 
     4741 gtccatcttg gccacgtcag ccaaggcgta tttggtgaac tgtttggtga gttccgtcag 
     4801 gtacggcagc atgtctttgg tgaacctgag ttctacacgg ccctcaccct cccggtagat 
     4861 gattgtttgc acccagccgg taatcatcac actcggtctt ttccccttgc cattgggctc 
     4921 ttgggttaac cggacttccc gccgtttcag gcgcagggcc gcttctttga gctggttgta 
     4981 ggaagattcg atagggacac ccgccatcgt cgctatgtcc tccgccgtca ctgaatacat 
     5041 cacttcatcg gtgacaggct cgctcctctt cacctggcta atacaggcca gaacgatccg 
     5101 ctgttcctga acactgaggc gatacgcggc ctcgaccagg gcattgcttt tgtaaaccat 
     5161 tgggggtgag gccacgttcg acattccttg tgtataaggg gacactgtat ctgcgtccca 
     5221 caatacaaca aatccgtccc tttacaacaa caaatccgtc ccttcttaac aacaaatccg 
     5281 tcccttaatg gcaacaaatc cgtccctttt taaactctac aggccacgga ttacgtggcc 
     5341 tgtagacgtc ctaaaaggtt taaaagggaa aaggaagaaa agggtggaaa cgcaaaaaac 
     5401 gcaccactac gtggccccgt tggggccgca tttgtgcccc tgaaggggcg ggggaggcgt 
     5461 ctgggcaatc cccgttttac cagtccccta tcgccgcctg agagggcgca ggaagcgagt 
     5521 aatcagggta tcgaggcgga ttcacccttg gcgtccaacc agcggcacca gcggcgcctg 
     5581 agaggggcgc gcccagctgt ctagggcggc ggatttgtcc tactcaggag agcgttcacc 
     5641 gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga 
     5701 tgcct 
//
