Standard European
Vector Architecture 3.0

Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes

WHAT IS SEVA

The Standard European Vector Architecture (SEVA) platform is a web-based resource and a material clone repository to assist the choice of optimal plasmid vectors for de-constructing and re-constructing complex prokaryotic phenotypes.

The SEVA database (SEVA-DB) is a resource for implementation of a standard for physical assembly of vector plasmids and for their non-ambiguous nomenclature as well as the index for a repository of functional sequences and actual constructs available to the community. The database was designed to simplify the choice of a given vector for the sake of specific applications, in such a way the user can easily decide the best configuration of replication origins, antibiotic resistance and business segments.

The SEVA-DB adopts simple design concepts imported from Systems Engineering into vector architecture and development to facilitate the swapping of functional modules and the extension genome engineering options to microorganisms beyond typical Laboratory strains. This platform has been implemented in the Molecular Environmental Microbiology Laboratory of the Centro Nacional de Biotecnología (CSIC).

UNDERSTANDING THE STANDARD

The SEVA format involves a set of rules for the physical assembly of the three basic components of plasmid vectors (origin of replication, selection marker and the business cargo) as well as a fixed nomenclature for the designation of the corresponding constructs.

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BACKBONE MODULES & NOMENCLATURE

Besides the standardized layout of constructs the SEVA format calls for the unequivocal designation for each of the vectors with the nomenclature explained in detail in that section and the code summarized in the plasmid list section.

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FIND YOUR PLASMID

We are a growing community that keeps up to date thanks to our contributors, who provide a constant flow of new SEVA plasmids. This way we have become one of the biggest repositories available, bringing solutions to actual researchers’ needs.

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The complete info about the SEVA collection is described here:

SEVA 1.0
SEVA 2.0
SEVA 3.0

CONTRIBUTING

If you wish to contribute with your favourite module please click  here