If you wish to contribute with your favourite module please follow these simple rules:
1- Remove, if any, the following restriction sites from your sequence: HindIII, PstI, XbaI, BamHI, SmaI, KpnI, SacI, SalI, EcoRI, SfiI, SphI, AvrII, PshAI, SwaI, AscI, FseI, PacI, SpeI, SanDI and NotI.
2- Flank your module with the appropriate restriction sites:
– Origin of replication by FseI and AscI.
– Antibiotic marker: SwaI and PshA.
– Cargoes: PacI and SpeI. The major transcriptional flow encoded in this segment must follow the
direction PacI ➔ SpeI.
3- Adhere to Open Access policy and send your new construct (plasmid and strain) to us for inclusion in the SEVA database, where we will assign a code according the SEVA rules.
Contributors to the collection of SEVA vectors must be accredited by the curators of the platform to follow the standards specified above, stored in the SEVA repository and assigned a definitive code prior to public release. Users are discouraged to assign SEVA codes that are not explicitly endorsed by the SEVA-DB managers.