LOCUS       pSEVA237R-Pem7          3855 bp    DNA     circular     03-NOV-2014
FEATURES             Location/Qualifiers
     terminator      complement(3750..3854)
                     /label=T1
     misc_feature    complement(2877..3735)
                     /note="oriV"
     gene            complement(2214..2876)
                     /note="Rep"
     misc_feature    complement(1960..2205)
                     /label=oriT
     gene            1113..1928
                     /label=Km
     terminator      883..985
                     /label=T0
     promoter        10..76
                     /source="PEM7-Promoter.seq"
     misc_feature    complement(152..158)
                     /note="RBS"
     misc_feature    166..876
                     /note="mcherry"
     source          1..3855
                     /dnas_title="pSEVA237R-Pem7"
ORIGIN      
        1 TTAATtaaTT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA
       61 GGTGAGGAAC TAAACCCCTA GGCCGCGGCC GCGCGAATTC GAGCTCGGTA CCCGGGGATC
      121 CTCTAGAGTC GACCTGCAGG CATGCAAGCT Taggaggaaa aacatATGGT GAGCAAGGGC
      181 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC
      241 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC
      301 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC
      361 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC
      421 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG
      481 GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AAGACGGCGA GTTCATCTAC
      541 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC
      601 ATGGGCTGGG AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG
      661 ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT CAAGACCACC
      721 TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC
      781 ATCACCTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC CGAGGGCCGC
      841 CACTCCACCG GCGGCATGGA CGAGCTGTAC AAGTAAACTA GTCTTGGACT CCTGTTGATA
      901 GATCCAGTAA TGACCTCAGA ACTCCATCTG GATTTGTTCA GAACGCTCGG TTGCCGCCGG
      961 GCGTTTTTTA TTGGTGAGAA TCCAGGGGTC CCCAATAATT ACGATTTAAA TTTGTGTCTC
     1021 AAAATCTCTG ATGTTACATT GCACAAGATA AAAATATATC ATCATGAACA ATAAAACTGT
     1081 CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAGCGT GAAACGAGCT
     1141 GTAGCCGTCC GCGTCTGAAC AGCAACATGG ATGCGGATCT GTATGGCTAT AAATGGGCGC
     1201 GTGATAACGT GGGTCAGAGC GGCGCGACCA TTTATCGTCT GTATGGCAAA CCGGATGCGC
     1261 CGGAACTGTT TCTGAAACAT GGCAAAGGCA GCGTGGCGAA CGATGTGACC GATGAAATGG
     1321 TGCGTCTGAA CTGGCTGACC GAATTTATGC CGCTGCCGAC CATTAAACAT TTTATTCGCA
     1381 CCCCGGATGA TGCGTGGCTG CTGACCACCG CGATTCCGGG CAAAACCGCG TTTCAGGTGC
     1441 TGGAAGAATA TCCGGATAGC GGCGAAAACA TTGTGGATGC GCTGGCCGTG TTTCTGCGTC
     1501 GTCTGCATAG CATTCCGGTG TGCAACTGCC CGTTTAACAG CGATCGTGTG TTTCGTCTGG
     1561 CCCAGGCGCA GAGCCGTATG AACAACGGCC TGGTGGATGC GAGCGATTTT GATGATGAAC
     1621 GTAACGGCTG GCCGGTGGAA CAGGTGTGGA AAGAAATGCA TAAACTGCTG CCGTTTAGCC
     1681 CGGATAGCGT GGTGACCCAC GGCGATTTTA GCCTGGATAA CCTGATTTTC GATGAAGGCA
     1741 AACTGATTGG CTGCATTGAT GTGGGCCGTG TGGGCATTGC GGATCGTTAT CAGGATCTGG
     1801 CCATTCTGTG GAACTGCCTG GGCGAATTTA GCCCGAGCCT GCAAAAACGT CTGTTTCAGA
     1861 AATATGGCAT TGATAATCCG GATATGAACA AACTGCAATT TCATCTGATG CTGGATGAAT
     1921 TTTTCTAATA ATTAATTGGA Ccgcggtccg cgcgttgtcC TTTTCCGCTG CATAACCCTG
     1981 CTTCGGGGTC ATTATAGCGA TTTTTTCGGT ATATCCATCC TTTTTCGCAC GATATACAGG
     2041 ATTTTGCCAA AGGGTTCGTG TAGACTTTCC TTGGTGTATC CAACGGCGTC AGCCGGGCAG
     2101 GATAGGTGAA GTAGGCCCAC CCGCGAGCGG GTGTTCCTTC TTCACTGTCC CTTATTCGCA
     2161 CCTGGCGGTG CTCAACGGGA ATCCTGCTCT GCGAGGCTGG CCGTAGGCCG GCCCTACCGG
     2221 CGCGGCAGCG TTACCCGTGT CGGCGGCTCC AACGGCTCGC CATCGTCCAG AAAACACGGC
     2281 TCATCGGGCA TCGGCAGGCG CTGCTGCCCG CGCCGTTCCC ATTCCTCCGT TTCGGTCAAG
     2341 GCTGGCAGGT CTGGTTCCAT GCCCGGAATG CCGGGCTGGC TGGGCGGCTC CTCGCCGGGG
     2401 CCGGTCGGTA GTTGCTGCTC GCCCGGATAC AGGGTCGGGA TGCGGCGCAG GTCGCCATGC
     2461 CCCAACAGCG ATTCGTCCTG GTCGTCGTGA TCAACCACCA CGGCGGCACT GAACACCGAC
     2521 AGGCGCAACT GGTCGCGGGG CTGGCCCCAC GCCACGCGGT CATTGACCAC GTAGGCCGAC
     2581 ACGGTGCCGG GGCCGTTGAG CTTCACGACG GAGATCCAGC GCTCGGCCAC CAAGTCCTTG
     2641 ACTGCGTATT GGACCGTCCG CAAAGAACGT CCGATGAGCT TGGAAAGTGT CTTCTGGCTG
     2701 ACCACCACGG CGTTCTGGTG GCCCATCTGC GCCACGAGGT GATGCAGCAG CATTGCCGCC
     2761 GTGGGTTTCC TCGCAATAAG CCCGGCCCAC GCCTCATGCG CTTTGCGTTC CGTTTGCACC
     2821 CAGTGACCGG GCTTGTTCTT GGCTTGAATG CCGATTTCTC TGGACTGCGT GGCCATGCTT
     2881 ATCTCCATGC GGTAGGGgTG CCGCACGGTT GCGGCACCAT GCGCAATCAG CTGCAACTTT
     2941 TCGGCAGCGC GACAACAATT ATGCGTTGCG TAAAAGTGGC AGTCAATTAC AGATTTTCTT
     3001 TAACCTACGC AATGAGCTAT TGCGGGGGGT GCCGCAATGA GCTGTTGCGT ACCCCCCTTT
     3061 TTTAAGTTGT TGATTTTTAA GTCTTTCGCA TTTCGCCCTA TATCTAGTTC TTTGGTGCCC
     3121 AAAGAAGGGC ACCCCTGCGG GGTTCCCCCA CGCCTTCGGC GCGGCTCCCC CTCCGGCAAA
     3181 AAGTGGCCCC TCCGGGGCTT GTTGATCGAC TGCGCGGCCT TCGGCCTTGC CCAAGGTGGC
     3241 GCTGCCCCCT TGGAACCCCC GCACTCGCCG CCGTGAGGCT CGGGGGGCAG GCGGGCGGGC
     3301 TTCGCCcTTC GACTGCCCCC ACTCGCATAG GCTTGGGTCG TTCCAGGCGC GTCAAGGCCA
     3361 AGCCGCTGCG CGGTCGCTGC GCGAGCCTTG ACCCGCCTTC CACTTGGTGT CCAACCGGCA
     3421 AGCGAAGCGC GCAGGCCGCA GGCCGGAGGC TTTTCCCCAG AGAAAATTAA AAAAATTGAT
     3481 GGGGCAAGGC CGCAGGCCGC GCAGTTGGAG CCGGTGGGTA TGTGGTCGAA GGCTGGGTAG
     3541 CCGGTGGGCA ATCCCTGTGG TCAAGCTCGT GGGCAGGCGC AGCCTGTCCA TCAGCTTGTC
     3601 CAGCAGGGTT GTCCACGGGC CGAGCGAAGC GAGCCAGCCG GTGGCCGCTC GCGGCCATCG
     3661 TCCACATATC CACGGGCTGG CAAGGGAGCG CAgcgaccgc gcagggcgaa gcccggagag
     3721 caagcccgta gggggGGCGC GCCCAGCTGT CTAGGGCGGC GGATTTGTCC TACTCAGGAG
     3781 AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTTCGACT GAGCCTTTCG
     3841 TTTTATTTGA TGCCT
//