LOCUS pSEVA234G 5279 bp ds-DNA circular 04-FEB-2013 FEATURES Location/Qualifiers terminator complement(5174..5278) /label=T1 misc_feature 2537..3352 /label=Km misc_feature complement(3384..3629) /label=oriT terminator 2307..2409 /label=T0 misc_feature complement(3638..5159) /note="pBBR1-ORI" /label=origin of replication misc_feature complement(4301..5159) /label=oriV gene complement(3638..4300) /label=Rep CDS 89..1171 /dnas_title="lac repressor protein" /gene="lacIq" /codon_start=1 /transl_table=11 /product="lac repressor protein" /protein_id="AAA56744.1" /db_xref="GI:595784" /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" /label=lac repressor protein promoter 1403..1432 /note="The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter" /citation=[3] /label=trc promoter -10_signal 1426..1432 /gene="lacUV5" /label=lacUV5 protein_bind 1438..1458 /bound_moiety="lacIq repressor protein" /label=protein_bind -35_signal 1403..1408 /gene="trp" /label=trp gene 1554..2270 /gene="gfp" /label=gfp ORIGIN 1 TTAATTAATT GACACCATCG AATGGTGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC 61 GGAAGAGAGT CAATTCAGGG TGGTGAATGT GAAACCAGTA ACGTTATACG ATGTCGCAGA 121 GTATGCCGGT GTCTCTTATC AGACCGTTTC CCGCGTGGTG AACCAGGCCA GCCACGTTTC 181 TGCGAAAACG CGGGAAAAAG TGGAAGCGGC GATGGCGGAG CTGAATTACA TTCCCAACCG 241 CGTGGCACAA CAACTGGCGG GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT 301 GGCCCTGCAC GCGCCGTCGC AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG 361 TGCCAGCGTG GTGGTGTCGA TGGTAGAACG AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT 421 GCACAATCTT CTCGCGCAAC GCGTCAGTGG GCTGATCATT AACTATCCGC TGGATGACCA 481 GGATGCCATT GCTGTGGAAG CTGCCTGCAC TAATGTTCCG GCGTTATTTC TTGATGTCTC 541 TGACCAGACA CCCATCAACA GTATTATTTT CTCCCATGAA GACGGTACGC GACTGGGCGT 601 GGAGCATCTG GTCGCATTGG GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC 661 TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG GCATAAATAT CTCACTCGCA ATCAAATTCA 721 GCCGATAGCG GAACGGGAAG GCGACTGGAG TGCCATGTCC GGTTTTCAAC AAACCATGCA 781 AATGCTGAAT GAGGGCATCG TTCCCACTGC GATGCTGGTT GCCAACGATC AGATGGCGCT 841 GGGCGCAATG CGCGCCATTA CCGAGTCCGG GCTGCGCGTT GGTGCGGATA TCTCGGTAGT 901 GGGATACGAC GATACCGAAG ACAGCTCATG TTATATCCCG CCGTTAACCA CCATCAAACA 961 GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA 1021 GGCGGTGAAG GGCAATCAGC TGTTGCCCGT CTCACTGGTG AAAAGAAAAA CCACCCTGGC 1081 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG 1141 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTA AGTTAGCGCG 1201 AATTGATCTG GTTTGACAGC TTATCATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA 1261 GGCAGCCATC GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT 1321 CGCTCAAGGC GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG 1381 GCAAATATTC TGAAATGAGC TGTTGACAAT TAATCATCCG GCTCGTATAA TGTGTGGAAT 1441 TGTGAGCGGA TAACAATTTC ACACCCTAGG CCGCGGCCGC GCGAATTCGA GCTCGGTACC 1501 CGGGGATCCT CTAGAGTCGA CCTGCAGGCA TGCAAGCTTA GGAGGAAAAA CATATGAGTA 1561 AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT GGTGATGTTA 1621 ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC GGAAAACTTA 1681 CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGCCAACA CTTGTCACTA 1741 CTTTGACTTA TGGTGTTCAA TGCTTTTCAA GATACCCAGA TCATATGAAA CGGCATGACT 1801 TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG 1861 ACGGGAACTA CAAGACACGT GCTGAAGTCA AGTTTGAAGG TGATACCCTT GTTAATAGAA 1921 TCGAGTTAAA AGGTATTGAT TTTAAAGAAG ATGGAAACAT TCTTGGACAC AAATTGGAAT 1981 ACAACTATAA CTCACACAAT GTATACATCA TGGCAGACAA ACAAAAGAAT GGAATCAAAG 2041 TTAACTTCAA AATTAGACAC AACATTGAAG ATGGAAGCGT TCAACTAGCA GACCATTATC 2101 AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC AGACAACCAT TACCTGTCCA 2161 CACAATCTGC CCTTTCGAAA GATCCCAACG AAAAGAGAGA CCACATGGTC CTTCTTGAGT 2221 TTGTAACAGC TGCTGGGATT ACACATGGCA TGGATGAACT ATACAAATAG cattcgtatg 2281 tctgctattt cgcgtataga ACTAGTCTTG GACTCCTGTT GATAGATCCA GTAATGACCT 2341 CAGAACTCCA TCTGGATTTG TTCAGAACGC TCGGTTGCCG CCGGGCGTTT TTTATTGGTG 2401 AGAATCCAGG GGTCCCCAAT AATTACGATT TAAATTTGTG TCTCAAAATC TCTGATGTTA 2461 CATTGCACAA GATAAAAATA TATCATCATG AACAATAAAA CTGTCTGCTT ACATAAACAG 2521 TAATACAAGG GGTGTTATGA GCCATATTCA GCGTGAAACG AGCTGTAGCC GTCCGCGTCT 2581 GAACAGCAAC ATGGATGCGG ATCTGTATGG CTATAAATGG GCGCGTGATA ACGTGGGTCA 2641 GAGCGGCGCG ACCATTTATC GTCTGTATGG CAAACCGGAT GCGCCGGAAC TGTTTCTGAA 2701 ACATGGCAAA GGCAGCGTGG CGAACGATGT GACCGATGAA ATGGTGCGTC TGAACTGGCT 2761 GACCGAATTT ATGCCGCTGC CGACCATTAA ACATTTTATT CGCACCCCGG ATGATGCGTG 2821 GCTGCTGACC ACCGCGATTC CGGGCAAAAC CGCGTTTCAG GTGCTGGAAG AATATCCGGA 2881 TAGCGGCGAA AACATTGTGG ATGCGCTGGC CGTGTTTCTG CGTCGTCTGC ATAGCATTCC 2941 GGTGTGCAAC TGCCCGTTTA ACAGCGATCG TGTGTTTCGT CTGGCCCAGG CGCAGAGCCG 3001 TATGAACAAC GGCCTGGTGG ATGCGAGCGA TTTTGATGAT GAACGTAACG GCTGGCCGGT 3061 GGAACAGGTG TGGAAAGAAA TGCATAAACT GCTGCCGTTT AGCCCGGATA GCGTGGTGAC 3121 CCACGGCGAT TTTAGCCTGG ATAACCTGAT TTTCGATGAA GGCAAACTGA TTGGCTGCAT 3181 TGATGTGGGC CGTGTGGGCA TTGCGGATCG TTATCAGGAT CTGGCCATTC TGTGGAACTG 3241 CCTGGGCGAA TTTAGCCCGA GCCTGCAAAA ACGTCTGTTT CAGAAATATG GCATTGATAA 3301 TCCGGATATG AACAAACTGC AATTTCATCT GATGCTGGAT GAATTTTTCT AATAATTAAT 3361 TGGACCGcgg tccgcgcgtt gtcCTTTTCC GCTGCATAAC CCTGCTTCGG GGTCATTATA 3421 GCGATTTTTT CGGTATATCC ATCCTTTTTC GCACGATATA CAGGATTTTG CCAAAGGGTT 3481 CGTGTAGACT TTCCTTGGTG TATCCAACGG CGTCAGCCGG GCAGGATAGG TGAAGTAGGC 3541 CCACCCGCGA GCGGGTGTTC CTTCTTCACT GTCCCTTATT CGCACCTGGC GGTGCTCAAC 3601 GGGAATCCTG CTCTGCGAGG CTGGCCGTAg gccggcccta ccggcgCGGC AGCGTTACCC 3661 GTGTCGGCGG CTCCAACGGC TCGCCATCGT CCAGAAAACA CGGCTCATCG GGCATCGGCA 3721 GGCGCTGCTG CCCGCGCCGT TCCCATTCCT CCGTTTCGGT CAAGGCTGGC AGGTCTGGTT 3781 CCATGCCCGG AATGCCGGGC TGGCTGGGCG GCTCCTCGCC GGGGCCGGTC GGTAGTTGCT 3841 GCTCGCCCGG ATACAGGGTC GGGATGCGGC GCAGGTCGCC ATGCCCCAAC AGCGATTCGT 3901 CCTGGTCGTC GTGATCAACC ACCACGGCGG CACTGAACAC CGACAGGCGC AACTGGTCGC 3961 GGGGCTGGCC CCACGCCACG CGGTCATTGA CCACGTAGGC CGACACGGTG CCGGGGCCGT 4021 TGAGCTTCAC GACGGAGATC CAGCGCTCGG CCACCAAGTC CTTGACTGCG TATTGGACCG 4081 TCCGCAAAGA ACGTCCGATG AGCTTGGAAA GTGTCTTCTG GCTGACCACC ACGGCGTTCT 4141 GGTGGCCCAT CTGCGCCACG AGGTGATGCA GCAGCATTGC CGCCGTGGGT TTCCTCGCAA 4201 TAAGCCCGGC CCACGCCTCA TGCGCTTTGC GTTCCGTTTG CACCCAGTGA CCGGGCTTGT 4261 TCTTGGCTTG AATGCCGATT TCTCTGGACT GCGTGGCCAT GCTTATCTCC ATGCGGTAGG 4321 GgTGCCGCAC GGTTGCGGCA CCATGCGCAA TCAGCTGCAA CTTTTCGGCA GCGCGACAAC 4381 AATTATGCGT TGCGTAAAAG TGGCAGTCAA TTACAGATTT TCTTTAACCT ACGCAATGAG 4441 CTATTGCGGG GGGTGCCGCA ATGAGCTGTT GCGTACCCCC CTTTTTTAAG TTGTTGATTT 4501 TTAAGTCTTT CGCATTTCGC CCTATATCTA GTTCTTTGGT GCCCAAAGAA GGGCACCCCT 4561 GCGGGGTTCC CCCACGCCTT CGGCGCGGCT CCCCCTCCGG CAAAAAGTGG CCCCTCCGGG 4621 GCTTGTTGAT CGACTGCGCG GCCTTCGGCC TTGCCCAAGG TGGCGCTGCC CCCTTGGAAC 4681 CCCCGCACTC GCCGCCGTGA GGCTCGGGGG GCAGGCGGGC GGGCTTCGCC cTTCGACTGC 4741 CCCCACTCGC ATAGGCTTGG GTCGTTCCAG GCGCGTCAAG GCCAAGCCGC TGCGCGGTCG 4801 CTGCGCGAGC CTTGACCCGC CTTCCACTTG GTGTCCAACC GGCAAGCGAA GCGCGCAGGC 4861 CGCAGGCCGG AGGCTTTTCC CCAGAGAAAA TTAAAAAAAT TGATGGGGCA AGGCCGCAGG 4921 CCGCGCAGTT GGAGCCGGTG GGTATGTGGT CGAAGGCTGG GTAGCCGGTG GGCAATCCCT 4981 GTGGTCAAGC TCGTGGGCAG GCGCAGCCTG TCCATCAGCT TGTCCAGCAG GGTTGTCCAC 5041 GGGCCGAGCG AAGCGAGCCA GCCGGTGGCC GCTCGCGGCC ATCGTCCACA TATCCACGGG 5101 CTGGCAAGGG AGCGCAgcga ccgcgcaggg cgaagcccgg agagcaagcc cgtagggggg 5161 gcgcgccCAG CTGTCTAGGG CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 5221 CAACAGATAA AACGAAAGGC CCAGTCTTTC GACTGAGCCT TTCGTTTTAT TTGATGCCT //