LOCUS pSEVA434 5308 bp ds-DNA circular 11-NOV-2013 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers terminator complement(5203..5307) /label=T1 /ApEinfo_fwdcolor="#808080" /ApEinfo_revcolor="#808080" CDS 2611..3402 /label=aadA gene /ApEinfo_fwdcolor="#808080" /ApEinfo_revcolor="#c0c0c0" -10_signal 2489..2495 /label=-10 region /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" -35_signal 2466..2471 /label=-35 sequence /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" misc_feature complement(3667..5188) /note="pBBR1-ORI" /label=origin of replication /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="#ff0000" misc_feature complement(3413..3658) /label=oriT /ApEinfo_fwdcolor="#808040" /ApEinfo_revcolor="#808040" misc_feature complement(4330..5188) /note="oriV" /label=oriV /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="#ff0000" misc_feature 2277..2379 /label=T0 /ApEinfo_fwdcolor="#c0c0c0" /ApEinfo_revcolor="#c0c0c0" gene complement(3667..4329) /note="Rep" /label=Rep /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" CDS 89..1171 /gene="lacIq" /codon_start=1 /transl_table=11 /product="lac repressor protein" /protein_id="AAA56744.1" /db_xref="GI:595784" /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" /label=lac repressor protein /ApEinfo_fwdcolor="DarkOrange1" /ApEinfo_revcolor="DarkOrange1" promoter 1403..1432 /note="The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter" /citation=[3] /label=The trc promoter combines the -35 region from the /ApEinfo_fwdcolor="seashell4" /ApEinfo_revcolor="seashell4" -10_signal 1426..1432 /gene="lacUV5" /label=lacUV5 /ApEinfo_fwdcolor="seashell4" /ApEinfo_revcolor="seashell4" protein_bind 1438..1458 /bound_moiety="lacIq repressor protein" /label=protein_bind /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" -35_signal 1403..1408 /gene="trp" /label=trp /ApEinfo_fwdcolor="seashell4" /ApEinfo_revcolor="seashell4" gene 1554..2270 /label=msGFP /ApEinfo_fwdcolor="#008080" /ApEinfo_revcolor="#008080" source 1..5308 /dnas_title="pSEVA434M" ORIGIN 1 TTAATTAATT GACACCATCG AATGGTGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC 61 GGAAGAGAGT CAATTCAGGG TGGTGAATGT GAAACCAGTA ACGTTATACG ATGTCGCAGA 121 GTATGCCGGT GTCTCTTATC AGACCGTTTC CCGCGTGGTG AACCAGGCCA GCCACGTTTC 181 TGCGAAAACG CGGGAAAAAG TGGAAGCGGC GATGGCGGAG CTGAATTACA TTCCCAACCG 241 CGTGGCACAA CAACTGGCGG GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT 301 GGCCCTGCAC GCGCCGTCGC AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG 361 TGCCAGCGTG GTGGTGTCGA TGGTAGAACG AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT 421 GCACAATCTT CTCGCGCAAC GCGTCAGTGG GCTGATCATT AACTATCCGC TGGATGACCA 481 GGATGCCATT GCTGTGGAAG CTGCCTGCAC TAATGTTCCG GCGTTATTTC TTGATGTCTC 541 TGACCAGACA CCCATCAACA GTATTATTTT CTCCCATGAA GACGGTACGC GACTGGGCGT 601 GGAGCATCTG GTCGCATTGG GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC 661 TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG GCATAAATAT CTCACTCGCA ATCAAATTCA 721 GCCGATAGCG GAACGGGAAG GCGACTGGAG TGCCATGTCC GGTTTTCAAC AAACCATGCA 781 AATGCTGAAT GAGGGCATCG TTCCCACTGC GATGCTGGTT GCCAACGATC AGATGGCGCT 841 GGGCGCAATG CGCGCCATTA CCGAGTCCGG GCTGCGCGTT GGTGCGGATA TCTCGGTAGT 901 GGGATACGAC GATACCGAAG ACAGCTCATG TTATATCCCG CCGTTAACCA CCATCAAACA 961 GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA 1021 GGCGGTGAAG GGCAATCAGC TGTTGCCCGT CTCACTGGTG AAAAGAAAAA CCACCCTGGC 1081 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG 1141 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCGCG 1201 AATTGATCTG GTTTGACAGC TTATCATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA 1261 GGCAGCCATC GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT 1321 CGCTCAAGGC GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG 1381 GCAAATATTC TGAAATGAGC TGTTGACAAT TAATCATCCG GCTCGTATAA TGTGTGGAAT 1441 TGTGAGCGGA TAACAATTTC ACACCCTAGG CCGCGGCCGC GCGAATTCGA GCTCGGTACC 1501 CGGGGATCCT CTAGAGTCGA CCTGCAGGCA TGCAAGCTTA GGAGGAAAAA CATAtgcgtA 1561 AAGGTGAAGA ACTGTTCACC GGTGTTGTTC CGATCCTGGT TGAACTGGAT GGTGATGTTA 1621 ACGGCCACAA ATTCTCTGTT CGTGGTGAAG GTGAAGGTGA TGCAACCAAC GGTAAACTGA 1681 CCCTGAAATT CATCTGCACT ACCGGTAAAC TGCCGGTTCC ATGGCCGACT CTGGTGACTA 1741 CCCTGACCTA TGGTGTTCAG TGTTTTTCTC GTTACCCGGA TCACATGAAG CAGCATGATT 1801 TCTTCAAATC TGCAATGCCG GAAGGTTATG TACAGGAGCG CACCATTTCT TTCAAAGACG 1861 ATGGCACCTA CAAAACCCGT GCAGAGGTTA AATTTGAAGG TGATACTCTG GTGAACCGTA 1921 TTGAACTGAA AGGCATTGAT TTCAAAGAGG ACGGCAACAT CCTGGGCCAC AAACTGGAAT 1981 ATAACTTCAA CTCCCATAAC GTTTACATCA CCGCAGACAA ACAGAAGAAC GGTATCAAAG 2041 CTAACTTCAA AATTCGCCAT AACGTTGAAG ACGGTAGCGT ACAGCTGGCG GACCACTACC 2101 AGCAGAACAC TCCGATCGGT GATGGTCCGG TTCTGCTGCC GGATAACCAC TACCTGTCCA 2161 CCCAGTCTAA ACTGTCCAAA GACCCGAACG AAAAGCGCGA CCACATGGTG CTGCTGGAGT 2221 TCGTTACTGC cGCAGGTATC ACGCACGGCA TGGATGAACT CTACAAATAA ACTAGTCTTG 2281 GACTCCTGTT GATAGATCCA GTAATGACCT CAGAACTCCA TCTGGATTTG TTCAGAACGC 2341 TCGGTTGCCG CCGGGCGTTT TTTATTGGTG AGAATCCAGG GGTCCCCAAT AATTACGATT 2401 TACGTATTTA AATgaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc 2461 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa 2521 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag 2581 ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg 2641 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc 2701 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg 2761 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt 2821 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt 2881 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga 2941 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat 3001 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg 3061 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta 3121 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg 3181 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac 3241 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct 3301 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc 3361 cactacgtga aaggcgagat caccaaggta gtcggcaaat aaGACCCTTG TCCTTTTCCG 3421 CTGCATAACC CTGCTTCGGG GTCATTATAG CGATTTTTTC GGTATATCCA TCCTTTTTCG 3481 CACGATATAC AGGATTTTGC CAAAGGGTTC GTGTAGACTT TCCTTGGTGT ATCCAACGGC 3541 GTCAGCCGGG CAGGATAGGT GAAGTAGGCC CACCCGCGAG CGGGTGTTCC TTCTTCACTG 3601 TCCCTTATTC GCACCTGGCG GTGCTCAACG GGAATCCTGC TCTGCGAGGC TGGCCGTAgg 3661 ccggccctac cggcgCGGCA GCGTTACCCG TGTCGGCGGC TCCAACGGCT CGCCATCGTC 3721 CAGAAAACAC GGCTCATCGG GCATCGGCAG GCGCTGCTGC CCGCGCCGTT CCCATTCCTC 3781 CGTTTCGGTC AAGGCTGGCA GGTCTGGTTC CATGCCCGGA ATGCCGGGCT GGCTGGGCGG 3841 CTCCTCGCCG GGGCCGGTCG GTAGTTGCTG CTCGCCCGGA TACAGGGTCG GGATGCGGCG 3901 CAGGTCGCCA TGCCCCAACA GCGATTCGTC CTGGTCGTCG TGATCAACCA CCACGGCGGC 3961 ACTGAACACC GACAGGCGCA ACTGGTCGCG GGGCTGGCCC CACGCCACGC GGTCATTGAC 4021 CACGTAGGCC GACACGGTGC CGGGGCCGTT GAGCTTCACG ACGGAGATCC AGCGCTCGGC 4081 CACCAAGTCC TTGACTGCGT ATTGGACCGT CCGCAAAGAA CGTCCGATGA GCTTGGAAAG 4141 TGTCTTCTGG CTGACCACCA CGGCGTTCTG GTGGCCCATC TGCGCCACGA GGTGATGCAG 4201 CAGCATTGCC GCCGTGGGTT TCCTCGCAAT AAGCCCGGCC CACGCCTCAT GCGCTTTGCG 4261 TTCCGTTTGC ACCCAGTGAC CGGGCTTGTT CTTGGCTTGA ATGCCGATTT CTCTGGACTG 4321 CGTGGCCATG CTTATCTCCA TGCGGTAGGG gTGCCGCACG GTTGCGGCAC CATGCGCAAT 4381 CAGCTGCAAC TTTTCGGCAG CGCGACAACA ATTATGCGTT GCGTAAAAGT GGCAGTCAAT 4441 TACAGATTTT CTTTAACCTA CGCAATGAGC TATTGCGGGG GGTGCCGCAA TGAGCTGTTG 4501 CGTACCCCCC TTTTTTAAGT TGTTGATTTT TAAGTCTTTC GCATTTCGCC CTATATCTAG 4561 TTCTTTGGTG CCCAAAGAAG GGCACCCCTG CGGGGTTCCC CCACGCCTTC GGCGCGGCTC 4621 CCCCTCCGGC AAAAAGTGGC CCCTCCGGGG CTTGTTGATC GACTGCGCGG CCTTCGGCCT 4681 TGCCCAAGGT GGCGCTGCCC CCTTGGAACC CCCGCACTCG CCGCCGTGAG GCTCGGGGGG 4741 CAGGCGGGCG GGCTTCGCCc TTCGACTGCC CCCACTCGCA TAGGCTTGGG TCGTTCCAGG 4801 CGCGTCAAGG CCAAGCCGCT GCGCGGTCGC TGCGCGAGCC TTGACCCGCC TTCCACTTGG 4861 TGTCCAACCG GCAAGCGAAG CGCGCAGGCC GCAGGCCGGA GGCTTTTCCC CAGAGAAAAT 4921 TAAAAAAATT GATGGGGCAA GGCCGCAGGC CGCGCAGTTG GAGCCGGTGG GTATGTGGTC 4981 GAAGGCTGGG TAGCCGGTGG GCAATCCCTG TGGTCAAGCT CGTGGGCAGG CGCAGCCTGT 5041 CCATCAGCTT GTCCAGCAGG GTTGTCCACG GGCCGAGCGA AGCGAGCCAG CCGGTGGCCG 5101 CTCGCGGCCA TCGTCCACAT ATCCACGGGC TGGCAAGGGA GCGCAgcgac cgcgcagggc 5161 gaagcccgga gagcaagccc gtaggggggg cgcgccCAGC TGTCTAGGGC GGCGGATTTG 5221 TCCTACTCAG GAGAGCGTTC ACCGACAAAC AACAGATAAA ACGAAAGGCC CAGTCTTTCG 5281 ACTGAGCCTT TCGTTTTATT TGATGCCT //