LOCUS pSEVA237M 3811 bp ds-DNA circular 08-MAY-2014 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers terminator complement(6742..6846) /label=T1 /ApEinfo_fwdcolor="#808080" /ApEinfo_revcolor="#808080" gene complement(5206..5868) /label=REP /ApEinfo_fwdcolor="#ff0080" /ApEinfo_revcolor="#ff0080" gene 4105..4920 /label=neo /ApEinfo_fwdcolor="#808080" /ApEinfo_revcolor="#808080" misc_feature complement(4952..5197) /label=oriT /ApEinfo_fwdcolor="#808040" /ApEinfo_revcolor="#808040" terminator 3875..3977 /label=T0 /ApEinfo_fwdcolor="#c0c0c0" /ApEinfo_revcolor="#c0c0c0" misc_feature 5869..6727 /note="oriV" misc_feature 5206..6727 /note="pBBR1 ori" CDS 4105..4920 /note="neomycin phosphotransferase" gene 3152..3868 /label=msfGFP /ApEinfo_fwdcolor="#008080" /ApEinfo_revcolor="#008080" CDS 3152..3868 /note="msfGFP" CDS complement(9..2666) /label=alkS crc-mut misc_feature complement(2669..2692) /label=tir promoter complement(2693..2798) /label=km promoter (pBAM1) misc_feature complement(2799..2948) /label=buffer seq primer_bind 2949..2972 /label=R24 promoter 3053..3053 /label=PalkB +1 misc_feature 3042..3047 /label=-10 misc_feature 3020..3025 /label=-35 misc_feature 3002..3021 /label=alkSb source 1..6847 /dnas_title="pSEVA239M" ORIGIN 1 TTAATTAAtc agataacacc ctgacgttcg gcttcaatta ttgcttgcgt gcgactcttt 61 acattaagga tggtaaatat tttcctcata tgccacttta tagcatcttc ggtgacatac 121 atctttgttg ctatttgttt gtttgagcac ccctctttta caagtttcaa gacactgacc 181 tgcttcagtg tcaaaaaatc accagcttta ttttctgcgg attttctaat ctccaccatt 241 tgcggcagac taaaagctcc aatcgcttga tctaaattaa ctgctgcgaa gacttcacat 301 gaagtcggta ttaatctctt aatcaaacgt atttcatcca gcactgtttg aaagcattga 361 agctgttttg ccatctccaa tgcataagca atgttatgct gagccttctt taatgtgccg 421 ccacctgctt gcgctccggc taaatacaat aagccacgga cttccaattg gcctgcgcta 481 attttacggg cttgctgaat agccaatagc gctctgtgcg cggcaagatg aaagttctga 541 tctcgggaaa gcgcgagtga ttgaacaagc agcaggcgtg cctttagggg ggctgagtgc 601 ggtccggaga aaatcttgcg atcttcaaag gcttgtaacg tatttatgta cgttacgcct 661 tgaaagagca agcgttgata gatctcaatt tggctcataa cttccaatct tggtagattt 721 ttttcagccg catatgcctt cgcccactcc aaaatctcaa tggaaccatt caggtcattc 781 cttccaagcc gccaagctga cacaacacgg catacggaaa aaaacacgtc tgtcaccccg 841 tgattggcaa taaactctaa aatttcggag aacttttctt ctgaggtgtc caagcggcac 901 aattcataat ttgactcaag ctccagagcg tccagcattt tcgaggtaaa cgcggtctcc 961 ataatctgcg cgccactgtc tgtgcgtgct tgagttataa gctgcctggc ccagctcatt 1021 ttcccgcttg ctagcgcttg ttgcagcttg gcgacataca gccaaccgaa agcaaaatct 1081 tgttttgcaa atttattcac ggcttgggcc tgagccagca ccttctctaa ctctgcaaat 1141 ctatactcac tggcaaaaat aaaagccaag caggttagcg cggccccttt cccaactgcg 1201 tttgaatccc caaataaact aatccactta ttacacagct cctcactcaa aagcatttca 1261 tcattcgttg ctttacctat tgcaaacaca agctgcggcc atcctttttc ttgccatttg 1321 ttttttttat cgggcggggt aattaggtct ttaagtaact tctctgctcg cgcgctttgc 1381 tgactgaaat acaataccca cgcgaaacaa ataagtacta tggggttctt gtgcaagtcc 1441 ttcactggca gctccgacag ccagtgtctc agcgtatcta tttcgccctg acgaaatgac 1501 aaatctaaaa ttattccctc actcatgccg acagcccagc gacagtcatt cgcccgtagg 1561 gctattcgta ttgcgtactg gtattcgcct ctacgccaat gccagaaagc tgcgcgctta 1621 agcaagtagg atcttttagc aggattttca gcccaagcaa ttccttgaag aaaatcgcgc 1681 aatactggat gtagtgtaaa ctgcgccggc tcaccttcta catggcgaag taacatgtaa 1741 ttcgtgctca gatatttaat acatgaggcc gcattgacgc atttgaatac ataattgtag 1801 ccatcaggcg tcacgacatc gagcaatgaa gaatttacaa gaaaaacacg ataccgctcg 1861 ggaagcgcct caaatatttc atcattaaag taattgtcta tttcaactac tgttgaaata 1921 gacttggccg gcaactcaca ttttaacaaa aacccgataa gaacaggcca cccctccatt 1981 tcttgcacca aggcctctat ctgtacctca gaaactccaa gaacagcctc tgcctccact 2041 aacgccactg cctcgtctgc gctaaaggcc aaatctttct cggtgtgctc ctgcatagcg 2101 cctgcaagtt taagccgcga gaaccctttt attgtattac ctgcgactgc aaacctgata 2161 ctttttggtg tttctaacat gaactccata agcgcgtgca acaacggcaa gcccaagccc 2221 tgattaatat tatccaaaca aattagcgtt tctatttcgt tactcgaggt actctgccaa 2281 agactggatg caaggtctcg caagagcgta ggcttgctca caccctctct cacatggctg 2341 aatgttacca gttcgaacgc ttcaagctgc tcaataattt ctgcgcagat atcaaattca 2401 ctgtaagaac tggctcttaa agaaatccac accgcagggc gtccggttgt tctgtggcgt 2461 agccactcga acgcaagagc cacggttttt ccatatccag gcggggctct ataaaggcat 2521 actctgggtg cagttccatc cgcgatactc aatctgggcc gatatatgca gttatgcact 2581 ttggcactta ctagagtcgt aattcgatcc agcccgatct tggcaaccgg gaaatcattG 2641 ttGatGatCt tGatCttcat gcccatggtt tttcctcctt ataaagttaa tcAACACCCC 2701 TTGTATTACT GTTTATGTAA GCAGACAGTT TTATTGTTCA TGATGATATA TTTTTATCTT 2761 GTGCAATGTA ACATCAGAGA TTTTGAGACA CAAGACGTGG CAAAAAACAT TATCCAGAAC 2821 GGGAGTGCGC CTTGAGCGAC ACGAATTATG CAGTGATTTA CGACCTGCAC AGCCATACCA 2881 CAGCTTCCGA TGGCTGCCTG ACGCCAGAAG CATTGGTGCA CCGTGCAGTC GATGATAAGC 2941 TGTCAAACAG CGGATAACAA TTTCACACAG GACGTGTTTT TCCAGCAGAC GACGGAGCAA 3001 AAACTACCCG TAGGTGTAGT TGGCGCAAGC GTCCGATTAG CTCAGGTTTA AGATGTCGAG 3061 AGCCTAGGCC GCGGCCGCGC GAATTCGAGC TCGGTACCCG GGGATCCTCT AGAGTCGACC 3121 TGCAGGCATG CAAGCTTAGG AGGAAAAACA TAtgcgtAAA GGTGAAGAAC TGTTCACCGG 3181 TGTTGTTCCG ATCCTGGTTG AACTGGATGG TGATGTTAAC GGCCACAAAT TCTCTGTTCG 3241 TGGTGAAGGT GAAGGTGATG CAACCAACGG TAAACTGACC CTGAAATTCA TCTGCACTAC 3301 CGGTAAACTG CCGGTTCCAT GGCCGACTCT GGTGACTACC CTGACCTATG GTGTTCAGTG 3361 TTTTTCTCGT TACCCGGATC ACATGAAGCA GCATGATTTC TTCAAATCTG CAATGCCGGA 3421 AGGTTATGTA CAGGAGCGCA CCATTTCTTT CAAAGACGAT GGCACCTACA AAACCCGTGC 3481 AGAGGTTAAA TTTGAAGGTG ATACTCTGGT GAACCGTATT GAACTGAAAG GCATTGATTT 3541 CAAAGAGGAC GGCAACATCC TGGGCCACAA ACTGGAATAT AACTTCAACT CCCATAACGT 3601 TTACATCACC GCAGACAAAC AGAAGAACGG TATCAAAGCT AACTTCAAAA TTCGCCATAA 3661 CGTTGAAGAC GGTAGCGTAC AGCTGGCGGA CCACTACCAG CAGAACACTC CGATCGGTGA 3721 TGGTCCGGTT CTGCTGCCGG ATAACCACTA CCTGTCCACC CAGTCTAAAC TGTCCAAAGA 3781 CCCGAACGAA AAGCGCGACC ACATGGTGCT GCTGGAGTTC GTTACTGCcG CAGGTATCAC 3841 GCACGGCATG GATGAACTCT ACAAATAAAC TAGTCTTGGA CTCCTGTTGA TAGATCCAGT 3901 AATGACCTCA GAACTCCATC TGGATTTGTT CAGAACGCTC GGTTGCCGCC GGGCGTTTTT 3961 TATTGGTGAG AATCCAGGGG TCCCCAATAA TTACGATTTA AATTTGTGTC TCAAAATCTC 4021 TGATGTTACA TTGCACAAGA TAAAAATATA TCATCATGAA CAATAAAACT GTCTGCTTAC 4081 ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAGC GTGAAACGAG CTGTAGCCGT 4141 CCGCGTCTGA ACAGCAACAT GGATGCGGAT CTGTATGGCT ATAAATGGGC GCGTGATAAC 4201 GTGGGTCAGA GCGGCGCGAC CATTTATCGT CTGTATGGCA AACCGGATGC GCCGGAACTG 4261 TTTCTGAAAC ATGGCAAAGG CAGCGTGGCG AACGATGTGA CCGATGAAAT GGTGCGTCTG 4321 AACTGGCTGA CCGAATTTAT GCCGCTGCCG ACCATTAAAC ATTTTATTCG CACCCCGGAT 4381 GATGCGTGGC TGCTGACCAC CGCGATTCCG GGCAAAACCG CGTTTCAGGT GCTGGAAGAA 4441 TATCCGGATA GCGGCGAAAA CATTGTGGAT GCGCTGGCCG TGTTTCTGCG TCGTCTGCAT 4501 AGCATTCCGG TGTGCAACTG CCCGTTTAAC AGCGATCGTG TGTTTCGTCT GGCCCAGGCG 4561 CAGAGCCGTA TGAACAACGG CCTGGTGGAT GCGAGCGATT TTGATGATGA ACGTAACGGC 4621 TGGCCGGTGG AACAGGTGTG GAAAGAAATG CATAAACTGC TGCCGTTTAG CCCGGATAGC 4681 GTGGTGACCC ACGGCGATTT TAGCCTGGAT AACCTGATTT TCGATGAAGG CAAACTGATT 4741 GGCTGCATTG ATGTGGGCCG TGTGGGCATT GCGGATCGTT ATCAGGATCT GGCCATTCTG 4801 TGGAACTGCC TGGGCGAATT TAGCCCGAGC CTGCAAAAAC GTCTGTTTCA GAAATATGGC 4861 ATTGATAATC CGGATATGAA CAAACTGCAA TTTCATCTGA TGCTGGATGA ATTTTTCTAA 4921 TAATTAATTG GACCGcggtc cgcgcgttgt cCTTTTCCGC TGCATAACCC TGCTTCGGGG 4981 TCATTATAGC GATTTTTTCG GTATATCCAT CCTTTTTCGC ACGATATACA GGATTTTGCC 5041 AAAGGGTTCG TGTAGACTTT CCTTGGTGTA TCCAACGGCG TCAGCCGGGC AGGATAGGTG 5101 AAGTAGGCCC ACCCGCGAGC GGGTGTTCCT TCTTCACTGT CCCTTATTCG CACCTGGCGG 5161 TGCTCAACGG GAATCCTGCT CTGCGAGGCT GGCCGTAGGC CGGCCCTACC GGCGCGGCAG 5221 CGTTACCCGT GTCGGCGGCT CCAACGGCTC GCCATCGTCC AGAAAACACG GCTCATCGGG 5281 CATCGGCAGG CGCTGCTGCC CGCGCCGTTC CCATTCCTCC GTTTCGGTCA AGGCTGGCAG 5341 GTCTGGTTCC ATGCCCGGAA TGCCGGGCTG GCTGGGCGGC TCCTCGCCGG GGCCGGTCGG 5401 TAGTTGCTGC TCGCCCGGAT ACAGGGTCGG GATGCGGCGC AGGTCGCCAT GCCCCAACAG 5461 CGATTCGTCC TGGTCGTCGT GATCAACCAC CACGGCGGCA CTGAACACCG ACAGGCGCAA 5521 CTGGTCGCGG GGCTGGCCCC ACGCCACGCG GTCATTGACC ACGTAGGCCG ACACGGTGCC 5581 GGGGCCGTTG AGCTTCACGA CGGAGATCCA GCGCTCGGCC ACCAAGTCCT TGACTGCGTA 5641 TTGGACCGTC CGCAAAGAAC GTCCGATGAG CTTGGAAAGT GTCTTCTGGC TGACCACCAC 5701 GGCGTTCTGG TGGCCCATCT GCGCCACGAG GTGATGCAGC AGCATTGCCG CCGTGGGTTT 5761 CCTCGCAATA AGCCCGGCCC ACGCCTCATG CGCTTTGCGT TCCGTTTGCA CCCAGTGACC 5821 GGGCTTGTTC TTGGCTTGAA TGCCGATTTC TCTGGACTGC GTGGCCATGC TTATCTCCAT 5881 GCGGTAGGGg TGCCGCACGG TTGCGGCACC ATGCGCAATC AGCTGCAACT TTTCGGCAGC 5941 GCGACAACAA TTATGCGTTG CGTAAAAGTG GCAGTCAATT ACAGATTTTC TTTAACCTAC 6001 GCAATGAGCT ATTGCGGGGG GTGCCGCAAT GAGCTGTTGC GTACCCCCCT TTTTTAAGTT 6061 GTTGATTTTT AAGTCTTTCG CATTTCGCCC TATATCTAGT TCTTTGGTGC CCAAAGAAGG 6121 GCACCCCTGC GGGGTTCCCC CACGCCTTCG GCGCGGCTCC CCCTCCGGCA AAAAGTGGCC 6181 CCTCCGGGGC TTGTTGATCG ACTGCGCGGC CTTCGGCCTT GCCCAAGGTG GCGCTGCCCC 6241 CTTGGAACCC CCGCACTCGC CGCCGTGAGG CTCGGGGGGC AGGCGGGCGG GCTTCGCCcT 6301 TCGACTGCCC CCACTCGCAT AGGCTTGGGT CGTTCCAGGC GCGTCAAGGC CAAGCCGCTG 6361 CGCGGTCGCT GCGCGAGCCT TGACCCGCCT TCCACTTGGT GTCCAACCGG CAAGCGAAGC 6421 GCGCAGGCCG CAGGCCGGAG GCTTTTCCCC AGAGAAAATT AAAAAAATTG ATGGGGCAAG 6481 GCCGCAGGCC GCGCAGTTGG AGCCGGTGGG TATGTGGTCG AAGGCTGGGT AGCCGGTGGG 6541 CAATCCCTGT GGTCAAGCTC GTGGGCAGGC GCAGCCTGTC CATCAGCTTG TCCAGCAGGG 6601 TTGTCCACGG GCCGAGCGAA GCGAGCCAGC CGGTGGCCGC TCGCGGCCAT CGTCCACATA 6661 TCCACGGGCT GGCAAGGGAG CGCAgcgacc gcgcagggcg aagcccggag agcaagcccg 6721 tagggggGGC GCGCCCAGCT GTCTAGGGCG GCGGATTTGT CCTACTCAGG AGAGCGTTCA 6781 CCGACAAACA ACAGATAAAA CGAAAGGCCC AGTCTTTCGA CTGAGCCTTT CGTTTTATTT 6841 GATGCCT //