LOCUS SEVA238-gfpC 5766 bp DNA circular SYN 18-MAY-2017 DEFINITION gfp without own RBS cloned into HindIII and SpeI sites (C-terminal) of SEVA238 to allow translation phusions with target proteins ACCESSION SEVA238-gfpC KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5766) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software FEATURES Location/Qualifiers CDS complement(19..984) /gene="xylS" /codon_start=1 /transl_table=11 /product="XylS" /protein_id="AGR55416.1" /db_xref="GI:525567874" /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAG RLHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGEL LLLNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDG FINLLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQ FIEENLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPS ANVRSITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA" misc_feature 1925..1977 /gene="Pm promoter" /product="benzoate inducible promoter" CDS 2071..2784 /gene="msf gfp" /product="monomeric superfolded gfp" /transl_table=11 CDS 2785..2787 /gene="msf gfp" /product="monomeric superfolded gfp" /transl_table=11 misc_signal 2785..2787 /label=STOP misc_feature 2794..2896 /gene="T0" /product="terminator" CDS 3024..3839 /gene="neo" /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AGR55417.1" /db_xref="GI:525567875" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" misc_feature 3881..4116 /note="oriT" CDS complement(4125..4787) /gene="rep" /codon_start=1 /transl_table=11 /product="replication protein" /protein_id="AGR55418.1" /db_xref="GI:525567876" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP RR" misc_feature 5661..5765 /gene="T1" /product="terminator" ORIGIN 1 ttaattaaac gttcgtaatc aagccacttc ctttttgcat tgacgcaggg tgtcggaagg 61 caactcgccg aacgcgctcc tatagttttc agcgaagcgt cccaaatgta agaagccgta 121 gtctagggct atctcagtta tactacgcac attggcactg ggatcgttca agcaggcgcg 181 gatgctttcg agcttgcggt tgcggatgta gttcttcggc gtggtgccgg cgtgcttctc 241 gaacaaattg tagagcgagc gtggactcat catcgccagc tccgctaacc gctcaaggct 301 gatattccgt ttgagattct cctcaatgaa ttgaacgact cgctcgaaag acgggttacc 361 tttgctgaaa atttcacggc tgacattgct gcccagcatt tcgagcagct tggaagcgat 421 gatccccgca tagtgctctt ggacccgagg catcgacttt gtatgttccg cttcgtcaca 481 aactaacccg agtagattga taaagccatc gagttgctgg agattgtgtc gcgcggcgaa 541 acggataccc tccctcggct tgtgccaatt gttgtcactg cacgcccgat caaggaccac 601 tgagggcaat ttaacgataa atttctcgca atcttctgaa taggtcaggt cggcttggtc 661 atccggattg agcagcaata gttcgcccgg cgcaaaatag tgctcctggc catggccacg 721 ccacaggcaa tggcctttga gtattatttg cagatgataa caggtctcta atccaggcga 781 gattaccctc acgctaccgc cgtagctgat tcgacacaga tcgaggcatc cgaagattct 841 gtggtgcagc ctgcctgccg ggcgcccgcc cttgggcagg cgaatagagt gcgtaccgac 901 atactggtta acataatcgg agactgcata gggctcggcg tggacgaaga tctgactttt 961 ctcgttcaat aagcaaaaat ccatagttca cggttctctt attttaatgt gggctgcttg 1021 gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct cgacgtgatg cgtatacggg 1081 ttacccccat tgccacgttg cgccatcctt tttgcaatca gtgaccactt ttccaagcaa 1141 aaataacgcc aagcagaacg aagacgttct ttttaagaag cgagaacacc agaagttcgt 1201 gctgtcgggg catggggcga cgaattggcg gataaagggg atctgctgga tattacggcc 1261 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt 1321 gcccgcctga tgaatgctca tccgtaatta cgtatggcaa tgaaagacgg tgagctggtg 1381 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca 1441 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat 1501 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt 1561 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg 1621 gacaacttct tcgcccccgt tttcaccatg catgggaatt agcttgatct gaccaacgac 1681 cggtagcgga gctatccaac ggcggtatac caggaaaaca cacagcaggt acatcagaac 1741 agtaccatga ctgaagaaca aatagttttt tcctgatcca taaagcagaa cggcctgctc 1801 catgacaaat ctggctcccc aactaatgcc ccatgcagcc agcataacca gcataaagtg 1861 cagtgtccgg tttgataggg ataagtccag ccttgcaaga agcggataca ggagtgcaaa 1921 aaatggctat ctctagtaag gcctacccct taggctttat gcaacagaaa caataataat 1981 ggagtcatga ccatgcctag gccgcggccg cgcgaattcg agctcggtac ccggggatcc 2041 tctagagtcg acctgcaggc atgcaagctt atgagtaaag gagaagaact tttcaccggt 2101 gttgttccga tcctggttga actggatggt gatgttaacg gccacaaatt ctctgttcgt 2161 ggtgaaggtg aaggtgatgc aaccaacggt aaactgaccc tgaaattcat ctgcactacc 2221 ggtaaactgc cggttccatg gccgactctg gtgactaccc tgacctatgg tgttcagtgt 2281 ttttctcgtt acccggatca catgaagcag catgatttct tcaaatctgc aatgccggaa 2341 ggttatgtac aggagcgcac catttctttc aaagacgatg gcacctacaa aacccgtgca 2401 gaggttaaat ttgaaggtga tactctggtg aaccgtattg aactgaaagg cattgatttc 2461 aaagaggacg gcaacatcct gggccacaaa ctggaatata acttcaactc ccataacgtt 2521 tacatcaccg cagacaaaca gaagaacggt atcaaagcta acttcaaaat tcgccataac 2581 gttgaagacg gtagcgtaca gctggcggac cactaccagc agaacactcc gatcggtgat 2641 ggtccggttc tgctgccgga taaccactac ctgtccaccc agtctaaact gtccaaagac 2701 ccgaacgaaa agcgcgacca catggtgctg ctggagttcg ttactgccgc aggtatcacg 2761 cacggcatgg atgaactata caaatagact agtcttggac tcctgttgat agatccagta 2821 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt 2881 attggtgaga atccaggggt ccccaataat tacgatttaa atttgtgtct caaaatctct 2941 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca 3001 taaacagtaa tacaaggggt gttatgagcc atattcagcg tgaaacgagc tgtagccgtc 3061 cgcgtctgaa cagcaacatg gatgcggatc tgtatggcta taaatgggcg cgtgataacg 3121 tgggtcagag cggcgcgacc atttatcgtc tgtatggcaa accggatgcg ccggaactgt 3181 ttctgaaaca tggcaaaggc agcgtggcga acgatgtgac cgatgaaatg gtgcgtctga 3241 actggctgac cgaatttatg ccgctgccga ccattaaaca ttttattcgc accccggatg 3301 atgcgtggct gctgaccacc gcgattccgg gcaaaaccgc gtttcaggtg ctggaagaat 3361 atccggatag cggcgaaaac attgtggatg cgctggccgt gtttctgcgt cgtctgcata 3421 gcattccggt gtgcaactgc ccgtttaaca gcgatcgtgt gtttcgtctg gcccaggcgc 3481 agagccgtat gaacaacggc ctggtggatg cgagcgattt tgatgatgaa cgtaacggct 3541 ggccggtgga acaggtgtgg aaagaaatgc ataaactgct gccgtttagc ccggatagcg 3601 tggtgaccca cggcgatttt agcctggata acctgatttt cgatgaaggc aaactgattg 3661 gctgcattga tgtgggccgt gtgggcattg cggatcgtta tcaggatctg gccattctgt 3721 ggaactgcct gggcgaattt agcccgagcc tgcaaaaacg tctgtttcag aaatatggca 3781 ttgataatcc ggatatgaac aaactgcaat ttcatctgat gctggatgaa tttttctaat 3841 aattaattgg accgcggtcc gcgcgttgtc cttttccgct gcataaccct gcttcggggt 3901 cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca 3961 aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga 4021 agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt 4081 gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc ggccctaccg gcgcggcagc 4141 gttacccgtg tcggcggctc caacggctcg ccatcgtcca gaaaacacgg ctcatcgggc 4201 atcggcaggc gctgctgccc gcgccgttcc cattcctccg tttcggtcaa ggctggcagg 4261 tctggttcca tgcccggaat gccgggctgg ctgggcggct cctcgccggg gccggtcggt 4321 agttgctgct cgcccggata cagggtcggg atgcggcgca ggtcgccatg ccccaacagc 4381 gattcgtcct ggtcgtcgtg atcaaccacc acggcggcac tgaacaccga caggcgcaac 4441 tggtcgcggg gctggcccca cgccacgcgg tcattgacca cgtaggccga cacggtgccg 4501 gggccgttga gcttcacgac ggagatccag cgctcggcca ccaagtcctt gactgcgtat 4561 tggaccgtcc gcaaagaacg tccgatgagc ttggaaagtg tcttctggct gaccaccacg 4621 gcgttctggt ggcccatctg cgccacgagg tgatgcagca gcattgccgc cgtgggtttc 4681 ctcgcaataa gcccggccca cgcctcatgc gctttgcgtt ccgtttgcac ccagtgaccg 4741 ggcttgttct tggcttgaat gccgatttct ctggactgcg tggccatgct tatctccatg 4801 cggtaggggt gccgcacggt tgcggcacca tgcgcaatca gctgcaactt ttcggcagcg 4861 cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta cagattttct ttaacctacg 4921 caatgagcta ttgcgggggg tgccgcaatg agctgttgcg tacccccctt ttttaagttg 4981 ttgattttta agtctttcgc atttcgccct atatctagtt ctttggtgcc caaagaaggg 5041 cacccctgcg gggttccccc acgccttcgg cgcggctccc cctccggcaa aaagtggccc 5101 ctccggggct tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg cgctgccccc 5161 ttggaacccc cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg cttcgccctt 5221 cgactgcccc cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc aagccgctgc 5281 gcggtcgctg cgcgagcctt gacccgcctt ccacttggtg tccaaccggc aagcgaagcg 5341 cgcaggccgc aggccggagg cttttcccca gagaaaatta aaaaaattga tggggcaagg 5401 ccgcaggccg cgcagttgga gccggtgggt atgtggtcga aggctgggta gccggtgggc 5461 aatccctgtg gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt ccagcagggt 5521 tgtccacggg ccgagcgaag cgagccagcc ggtggccgct cgcggccatc gtccacatat 5581 ccacgggctg gcaagggagc gcagcgaccg cgcagggcga agcccggaga gcaagcccgt 5641 agggggggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga gagcgttcac 5701 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg 5761 atgcct //