LOCUS SEVA238-gfpN 5801 bp DNA circular SYN 18-MAY-2017 DEFINITION monomeric superfolded gfp cloned into AvrII EcoRI sites (N terminus of polylinker) of SEVA238 to allow translation phusion with target protein ACCESSION SEVA238-gfpN KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5801) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software FEATURES Location/Qualifiers CDS complement(19..984) /gene="xylS" /codon_start=1 /transl_table=11 /product="XylS" /protein_id="AGR55416.1" /db_xref="GI:525567874" /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAG RLHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGEL LLLNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDG FINLLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQ FIEENLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPS ANVRSITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA" misc_feature 1925..1977 /gene="Pm promoter" /product="benzoate inducible promoter" misc_signal 2006..2011 /label=SEVA RBS CDS 2020..2733 /gene="msf gfp" /product="monomeric superfolded gfp" /transl_table=11 misc_feature 2829..2931 /gene="T0" /product="terminator" CDS 3059..3874 /gene="neo" /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AGR55417.1" /db_xref="GI:525567875" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" misc_feature 3916..4151 /note="oriT" CDS complement(4160..4822) /gene="rep" /codon_start=1 /transl_table=11 /product="replication protein" /protein_id="AGR55418.1" /db_xref="GI:525567876" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP RR" misc_feature 5696..5800 /gene="T1" /product="terminator" ORIGIN 1 ttaattaaac gttcgtaatc aagccacttc ctttttgcat tgacgcaggg tgtcggaagg 61 caactcgccg aacgcgctcc tatagttttc agcgaagcgt cccaaatgta agaagccgta 121 gtctagggct atctcagtta tactacgcac attggcactg ggatcgttca agcaggcgcg 181 gatgctttcg agcttgcggt tgcggatgta gttcttcggc gtggtgccgg cgtgcttctc 241 gaacaaattg tagagcgagc gtggactcat catcgccagc tccgctaacc gctcaaggct 301 gatattccgt ttgagattct cctcaatgaa ttgaacgact cgctcgaaag acgggttacc 361 tttgctgaaa atttcacggc tgacattgct gcccagcatt tcgagcagct tggaagcgat 421 gatccccgca tagtgctctt ggacccgagg catcgacttt gtatgttccg cttcgtcaca 481 aactaacccg agtagattga taaagccatc gagttgctgg agattgtgtc gcgcggcgaa 541 acggataccc tccctcggct tgtgccaatt gttgtcactg cacgcccgat caaggaccac 601 tgagggcaat ttaacgataa atttctcgca atcttctgaa taggtcaggt cggcttggtc 661 atccggattg agcagcaata gttcgcccgg cgcaaaatag tgctcctggc catggccacg 721 ccacaggcaa tggcctttga gtattatttg cagatgataa caggtctcta atccaggcga 781 gattaccctc acgctaccgc cgtagctgat tcgacacaga tcgaggcatc cgaagattct 841 gtggtgcagc ctgcctgccg ggcgcccgcc cttgggcagg cgaatagagt gcgtaccgac 901 atactggtta acataatcgg agactgcata gggctcggcg tggacgaaga tctgactttt 961 ctcgttcaat aagcaaaaat ccatagttca cggttctctt attttaatgt gggctgcttg 1021 gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct cgacgtgatg cgtatacggg 1081 ttacccccat tgccacgttg cgccatcctt tttgcaatca gtgaccactt ttccaagcaa 1141 aaataacgcc aagcagaacg aagacgttct ttttaagaag cgagaacacc agaagttcgt 1201 gctgtcgggg catggggcga cgaattggcg gataaagggg atctgctgga tattacggcc 1261 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt 1321 gcccgcctga tgaatgctca tccgtaatta cgtatggcaa tgaaagacgg tgagctggtg 1381 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca 1441 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat 1501 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt 1561 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg 1621 gacaacttct tcgcccccgt tttcaccatg catgggaatt agcttgatct gaccaacgac 1681 cggtagcgga gctatccaac ggcggtatac caggaaaaca cacagcaggt acatcagaac 1741 agtaccatga ctgaagaaca aatagttttt tcctgatcca taaagcagaa cggcctgctc 1801 catgacaaat ctggctcccc aactaatgcc ccatgcagcc agcataacca gcataaagtg 1861 cagtgtccgg tttgataggg ataagtccag ccttgcaaga agcggataca ggagtgcaaa 1921 aaatggctat ctctagtaag gcctacccct taggctttat gcaacagaaa caataataat 1981 ggagtcatga ccatgcctag gtagcaagag gaatatcata tgagtaaagg agaagaactt 2041 ttcaccggtg ttgttccgat cctggttgaa ctggatggtg atgttaacgg ccacaaattc 2101 tctgttcgtg gtgaaggtga aggtgatgca accaacggta aactgaccct gaaattcatc 2161 tgcactaccg gtaaactgcc ggttccatgg ccgactctgg tgactaccct gacctatggt 2221 gttcagtgtt tttctcgtta cccggatcac atgaagcagc atgatttctt caaatctgca 2281 atgccggaag gttatgtaca ggagcgcacc atttctttca aagacgatgg cacctacaaa 2341 acccgtgcag aggttaaatt tgaaggtgat actctggtga accgtattga actgaaaggc 2401 attgatttca aagaggacgg caacatcctg ggccacaaac tggaatataa cttcaactcc 2461 cataacgttt acatcaccgc agacaaacag aagaacggta tcaaagctaa cttcaaaatt 2521 cgccataacg ttgaagacgg tagcgtacag ctggcggacc actaccagca gaacactccg 2581 atcggtgatg gtccggttct gctgccggat aaccactacc tgtccaccca gtctaaactg 2641 tccaaagacc cgaacgaaaa gcgcgaccac atggtgctgc tggagttcgt tactgccgca 2701 ggtatcacgc acggcatgga tgaactatac aaagaattcg agctcggtac ccggggatcc 2761 tctagagtcg acctgcaggc atgcaagctt gcggccgcgt cgtgactggg aaaaccctgg 2821 cgactagtct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt 2881 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca ggggtcccca 2941 ataattacga tttaaatttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa 3001 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat 3061 gagccatatt cagcgtgaaa cgagctgtag ccgtccgcgt ctgaacagca acatggatgc 3121 ggatctgtat ggctataaat gggcgcgtga taacgtgggt cagagcggcg cgaccattta 3181 tcgtctgtat ggcaaaccgg atgcgccgga actgtttctg aaacatggca aaggcagcgt 3241 ggcgaacgat gtgaccgatg aaatggtgcg tctgaactgg ctgaccgaat ttatgccgct 3301 gccgaccatt aaacatttta ttcgcacccc ggatgatgcg tggctgctga ccaccgcgat 3361 tccgggcaaa accgcgtttc aggtgctgga agaatatccg gatagcggcg aaaacattgt 3421 ggatgcgctg gccgtgtttc tgcgtcgtct gcatagcatt ccggtgtgca actgcccgtt 3481 taacagcgat cgtgtgtttc gtctggccca ggcgcagagc cgtatgaaca acggcctggt 3541 ggatgcgagc gattttgatg atgaacgtaa cggctggccg gtggaacagg tgtggaaaga 3601 aatgcataaa ctgctgccgt ttagcccgga tagcgtggtg acccacggcg attttagcct 3661 ggataacctg attttcgatg aaggcaaact gattggctgc attgatgtgg gccgtgtggg 3721 cattgcggat cgttatcagg atctggccat tctgtggaac tgcctgggcg aatttagccc 3781 gagcctgcaa aaacgtctgt ttcagaaata tggcattgat aatccggata tgaacaaact 3841 gcaatttcat ctgatgctgg atgaattttt ctaataatta attggaccgc ggtccgcgcg 3901 ttgtcctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat 3961 ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg 4021 tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt 4081 tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga 4141 ggctggccgt aggccggccc taccggcgcg gcagcgttac ccgtgtcggc ggctccaacg 4201 gctcgccatc gtccagaaaa cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc 4261 gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg 4321 gctggctggg cggctcctcg ccggggccgg tcggtagttg ctgctcgccc ggatacaggg 4381 tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa 4441 ccaccacggc ggcactgaac accgacaggc gcaactggtc gcggggctgg ccccacgcca 4501 cgcggtcatt gaccacgtag gccgacacgg tgccggggcc gttgagcttc acgacggaga 4561 tccagcgctc ggccaccaag tccttgactg cgtattggac cgtccgcaaa gaacgtccga 4621 tgagcttgga aagtgtcttc tggctgacca ccacggcgtt ctggtggccc atctgcgcca 4681 cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc aataagcccg gcccacgcct 4741 catgcgcttt gcgttccgtt tgcacccagt gaccgggctt gttcttggct tgaatgccga 4801 tttctctgga ctgcgtggcc atgcttatct ccatgcggta ggggtgccgc acggttgcgg 4861 caccatgcgc aatcagctgc aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa 4921 agtggcagtc aattacagat tttctttaac ctacgcaatg agctattgcg gggggtgccg 4981 caatgagctg ttgcgtaccc ccctttttta agttgttgat ttttaagtct ttcgcatttc 5041 gccctatatc tagttctttg gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc 5101 ttcggcgcgg ctccccctcc ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg 5161 cggccttcgg ccttgcccaa ggtggcgctg cccccttgga acccccgcac tcgccgccgt 5221 gaggctcggg gggcaggcgg gcgggcttcg cccttcgact gcccccactc gcataggctt 5281 gggtcgttcc aggcgcgtca aggccaagcc gctgcgcggt cgctgcgcga gccttgaccc 5341 gccttccact tggtgtccaa ccggcaagcg aagcgcgcag gccgcaggcc ggaggctttt 5401 ccccagagaa aattaaaaaa attgatgggg caaggccgca ggccgcgcag ttggagccgg 5461 tgggtatgtg gtcgaaggct gggtagccgg tgggcaatcc ctgtggtcaa gctcgtgggc 5521 aggcgcagcc tgtccatcag cttgtccagc agggttgtcc acgggccgag cgaagcgagc 5581 cagccggtgg ccgctcgcgg ccatcgtcca catatccacg ggctggcaag ggagcgcagc 5641 gaccgcgcag ggcgaagccc ggagagcaag cccgtagggg gggcgcgccc agctgtctag 5701 ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag 5761 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc t //