LOCUS pSEVA234msfGFP 5249 bp ds-DNA circular 19-JUL-2017 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers terminator complement(5144..5248) /label=T1 /ApEinfo_fwdcolor="seashell4" /ApEinfo_revcolor="seashell4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(3354..3599) /label=oriT /ApEinfo_fwdcolor="maroon4" /ApEinfo_revcolor="maroon4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2277..2379 /label=T0 /ApEinfo_fwdcolor="maroon4" /ApEinfo_revcolor="maroon4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" gene 2507..3322 /label=Km /ApEinfo_fwdcolor="gray45" /ApEinfo_revcolor="gray45" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(3608..5129) /note="pBBR1-ORI" /label=pBBR1-ORI /ApEinfo_fwdcolor="maroon4" /ApEinfo_revcolor="maroon4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(4271..5129) /note="oriV" /label=oriV /ApEinfo_fwdcolor="maroon4" /ApEinfo_revcolor="maroon4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" gene complement(3608..4270) /note="Rep" /label=Rep /ApEinfo_fwdcolor="gray45" /ApEinfo_revcolor="gray45" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2583..2585 /label=-10 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(3387..3389) /label=-10(3) /ApEinfo_label="-10" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 9..14 /label=-35 /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 89..1171 /gene="lacIq" /codon_start=1 /transl_table=11 /product="lac repressor protein" /protein_id="AAA56744.1" /db_xref="GI:595784" /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" /label=lac repressor protein /ApEinfo_fwdcolor="DarkOrange1" /ApEinfo_revcolor="DarkOrange1" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(105..107) /label=-10(4) /ApEinfo_label="-10" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 1403..1432 /note="The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter" /citation=[3] /label=The trc promoter combines the -35 region from the /ApEinfo_fwdcolor="seashell4" /ApEinfo_revcolor="seashell4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" -10_signal 1426..1432 /gene="lacUV5" /label=lacUV5 /ApEinfo_fwdcolor="seashell4" /ApEinfo_revcolor="seashell4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" protein_bind 1438..1458 /bound_moiety="lacIq repressor protein" /label=protein_bind /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" -35_signal 1403..1408 /gene="trp" /label=trp /ApEinfo_fwdcolor="seashell4" /ApEinfo_revcolor="seashell4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_binding 1436..1458 /label=LacO /ApEinfo_fwdcolor="#6495ed" /ApEinfo_revcolor="#6495ed" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1427..1429 /label=-10(1) /ApEinfo_label="-10" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1403..1408 /label=-35(2) /ApEinfo_label="-35" /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1540..1546 /label=rbs /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="#ffff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1981..1983 /label=-10(2) /ApEinfo_label="-10" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1554..2270 /label=msfGFP (sin NheI9 /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" source 1..5249 /dnas_title="pSEVA234M" ORIGIN 1 TTAATTAATT GACACCATCG AATGGTGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC 61 GGAAGAGAGT CAATTCAGGG TGGTGAATGT GAAACCAGTA ACGTTATACG ATGTCGCAGA 121 GTATGCCGGT GTCTCTTATC AGACCGTTTC CCGCGTGGTG AACCAGGCCA GCCACGTTTC 181 TGCGAAAACG CGGGAAAAAG TGGAAGCGGC GATGGCGGAG CTGAATTACA TTCCCAACCG 241 CGTGGCACAA CAACTGGCGG GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT 301 GGCCCTGCAC GCGCCGTCGC AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG 361 TGCCAGCGTG GTGGTGTCGA TGGTAGAACG AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT 421 GCACAATCTT CTCGCGCAAC GCGTCAGTGG GCTGATCATT AACTATCCGC TGGATGACCA 481 GGATGCCATT GCTGTGGAAG CTGCCTGCAC TAATGTTCCG GCGTTATTTC TTGATGTCTC 541 TGACCAGACA CCCATCAACA GTATTATTTT CTCCCATGAA GACGGTACGC GACTGGGCGT 601 GGAGCATCTG GTCGCATTGG GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC 661 TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG GCATAAATAT CTCACTCGCA ATCAAATTCA 721 GCCGATAGCG GAACGGGAAG GCGACTGGAG TGCCATGTCC GGTTTTCAAC AAACCATGCA 781 AATGCTGAAT GAGGGCATCG TTCCCACTGC GATGCTGGTT GCCAACGATC AGATGGCGCT 841 GGGCGCAATG CGCGCCATTA CCGAGTCCGG GCTGCGCGTT GGTGCGGATA TCTCGGTAGT 901 GGGATACGAC GATACCGAAG ACAGCTCATG TTATATCCCG CCGTTAACCA CCATCAAACA 961 GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA 1021 GGCGGTGAAG GGCAATCAGC TGTTGCCCGT CTCACTGGTG AAAAGAAAAA CCACCCTGGC 1081 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG 1141 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCGCG 1201 AATTGATCTG GTTTGACAGC TTATCATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA 1261 GGCAGCCATC GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT 1321 CGCTCAAGGC GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG 1381 GCAAATATTC TGAAATGAGC TGTTGACAAT TAATCATCCG GCTCGTATAA TGTGTGGAAT 1441 TGTGAGCGGA TAACAATTTC ACACCCTAGG CCGCGGCCGC GCGAATTCGA GCTCGGTACC 1501 CGGGGATCCT CTAGAGTCGA CCTGCAGGCA TGCAAGCTTA GGAGGAAAAA CATATGCGTA 1561 AAGGTGAAGA ACTGTTCACC GGTGTTGTTC CGATCCTGGT TGAACTGGAT GGTGATGTTA 1621 ACGGCCACAA ATTCTCTGTT CGTGGTGAAG GTGAAGGTGA TGCAACCAAC GGTAAACTGA 1681 CCCTGAAATT CATCTGCACT ACCGGTAAAC TGCCGGTTCC ATGGCCGACT CTGGTGACTA 1741 CCCTGACCTA TGGTGTTCAG TGTTTTTCTC GTTACCCGGA TCACATGAAG CAGCATGATT 1801 TCTTCAAATC TGCAATGCCG GAAGGTTATG TACAGGAGCG CACCATTTCT TTCAAAGACG 1861 ATGGCACCTA CAAAACCCGT GCAGAGGTTA AATTTGAAGG TGATACTCTG GTGAACCGTA 1921 TTGAACTGAA AGGCATTGAT TTCAAAGAGG ACGGCAACAT CCTGGGCCAC AAACTGGAAT 1981 ATAACTTCAA CTCCCATAAC GTTTACATCA CCGCAGACAA ACAGAAGAAC GGTATCAAAG 2041 CTAACTTCAA AATTCGCCAT AACGTTGAAG ACGGTAGCGT ACAGCTGGCG GACCACTACC 2101 AGCAGAACAC TCCGATCGGT GATGGTCCGG TTCTGCTGCC GGATAACCAC TACCTGTCCA 2161 CCCAGTCTAA ACTGTCCAAA GACCCGAACG AAAAGCGCGA CCACATGGTG CTGCTGGAGT 2221 TCGTTACTGC CGCAGGTATC ACGCACGGCA TGGATGAACT CTACAAATAA ACTAGTCTTG 2281 GACTCCTGTT GATAGATCCA GTAATGACCT CAGAACTCCA TCTGGATTTG TTCAGAACGC 2341 TCGGTTGCCG CCGGGCGTTT TTTATTGGTG AGAATCCAGG GGTCCCCAAT AATTACGATT 2401 TAAATTTGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG 2461 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA 2521 GCGTGAAACG AGCTGTAGCC GTCCGCGTCT GAACAGCAAC ATGGATGCGG ATCTGTATGG 2581 CTATAAATGG GCGCGTGATA ACGTGGGTCA GAGCGGCGCG ACCATTTATC GTCTGTATGG 2641 CAAACCGGAT GCGCCGGAAC TGTTTCTGAA ACATGGCAAA GGCAGCGTGG CGAACGATGT 2701 GACCGATGAA ATGGTGCGTC TGAACTGGCT GACCGAATTT ATGCCGCTGC CGACCATTAA 2761 ACATTTTATT CGCACCCCGG ATGATGCGTG GCTGCTGACC ACCGCGATTC CGGGCAAAAC 2821 CGCGTTTCAG GTGCTGGAAG AATATCCGGA TAGCGGCGAA AACATTGTGG ATGCGCTGGC 2881 CGTGTTTCTG CGTCGTCTGC ATAGCATTCC GGTGTGCAAC TGCCCGTTTA ACAGCGATCG 2941 TGTGTTTCGT CTGGCCCAGG CGCAGAGCCG TATGAACAAC GGCCTGGTGG ATGCGAGCGA 3001 TTTTGATGAT GAACGTAACG GCTGGCCGGT GGAACAGGTG TGGAAAGAAA TGCATAAACT 3061 GCTGCCGTTT AGCCCGGATA GCGTGGTGAC CCACGGCGAT TTTAGCCTGG ATAACCTGAT 3121 TTTCGATGAA GGCAAACTGA TTGGCTGCAT TGATGTGGGC CGTGTGGGCA TTGCGGATCG 3181 TTATCAGGAT CTGGCCATTC TGTGGAACTG CCTGGGCGAA TTTAGCCCGA GCCTGCAAAA 3241 ACGTCTGTTT CAGAAATATG GCATTGATAA TCCGGATATG AACAAACTGC AATTTCATCT 3301 GATGCTGGAT GAATTTTTCT AATAATTAAT TGGACcgcgg tccgcgcgtt gtcCTTTTCC 3361 GCTGCATAAC CCTGCTTCGG GGTCATTATA GCGATTTTTT CGGTATATCC ATCCTTTTTC 3421 GCACGATATA CAGGATTTTG CCAAAGGGTT CGTGTAGACT TTCCTTGGTG TATCCAACGG 3481 CGTCAGCCGG GCAGGATAGG TGAAGTAGGC CCACCCGCGA GCGGGTGTTC CTTCTTCACT 3541 GTCCCTTATT CGCACCTGGC GGTGCTCAAC GGGAATCCTG CTCTGCGAGG CTGGCCGTAG 3601 GCCGGCCCTA CCGGCGCGGC AGCGTTACCC GTGTCGGCGG CTCCAACGGC TCGCCATCGT 3661 CCAGAAAACA CGGCTCATCG GGCATCGGCA GGCGCTGCTG CCCGCGCCGT TCCCATTCCT 3721 CCGTTTCGGT CAAGGCTGGC AGGTCTGGTT CCATGCCCGG AATGCCGGGC TGGCTGGGCG 3781 GCTCCTCGCC GGGGCCGGTC GGTAGTTGCT GCTCGCCCGG ATACAGGGTC GGGATGCGGC 3841 GCAGGTCGCC ATGCCCCAAC AGCGATTCGT CCTGGTCGTC GTGATCAACC ACCACGGCGG 3901 CACTGAACAC CGACAGGCGC AACTGGTCGC GGGGCTGGCC CCACGCCACG CGGTCATTGA 3961 CCACGTAGGC CGACACGGTG CCGGGGCCGT TGAGCTTCAC GACGGAGATC CAGCGCTCGG 4021 CCACCAAGTC CTTGACTGCG TATTGGACCG TCCGCAAAGA ACGTCCGATG AGCTTGGAAA 4081 GTGTCTTCTG GCTGACCACC ACGGCGTTCT GGTGGCCCAT CTGCGCCACG AGGTGATGCA 4141 GCAGCATTGC CGCCGTGGGT TTCCTCGCAA TAAGCCCGGC CCACGCCTCA TGCGCTTTGC 4201 GTTCCGTTTG CACCCAGTGA CCGGGCTTGT TCTTGGCTTG AATGCCGATT TCTCTGGACT 4261 GCGTGGCCAT GCTTATCTCC ATGCGGTAGG GgTGCCGCAC GGTTGCGGCA CCATGCGCAA 4321 TCAGCTGCAA CTTTTCGGCA GCGCGACAAC AATTATGCGT TGCGTAAAAG TGGCAGTCAA 4381 TTACAGATTT TCTTTAACCT ACGCAATGAG CTATTGCGGG GGGTGCCGCA ATGAGCTGTT 4441 GCGTACCCCC CTTTTTTAAG TTGTTGATTT TTAAGTCTTT CGCATTTCGC CCTATATCTA 4501 GTTCTTTGGT GCCCAAAGAA GGGCACCCCT GCGGGGTTCC CCCACGCCTT CGGCGCGGCT 4561 CCCCCTCCGG CAAAAAGTGG CCCCTCCGGG GCTTGTTGAT CGACTGCGCG GCCTTCGGCC 4621 TTGCCCAAGG TGGCGCTGCC CCCTTGGAAC CCCCGCACTC GCCGCCGTGA GGCTCGGGGG 4681 GCAGGCGGGC GGGCTTCGCC cTTCGACTGC CCCCACTCGC ATAGGCTTGG GTCGTTCCAG 4741 GCGCGTCAAG GCCAAGCCGC TGCGCGGTCG CTGCGCGAGC CTTGACCCGC CTTCCACTTG 4801 GTGTCCAACC GGCAAGCGAA GCGCGCAGGC CGCAGGCCGG AGGCTTTTCC CCAGAGAAAA 4861 TTAAAAAAAT TGATGGGGCA AGGCCGCAGG CCGCGCAGTT GGAGCCGGTG GGTATGTGGT 4921 CGAAGGCTGG GTAGCCGGTG GGCAATCCCT GTGGTCAAGC TCGTGGGCAG GCGCAGCCTG 4981 TCCATCAGCT TGTCCAGCAG GGTTGTCCAC GGGCCGAGCG AAGCGAGCCA GCCGGTGGCC 5041 GCTCGCGGCC ATCGTCCACA TATCCACGGG CTGGCAAGGG AGCGCAgcga ccgcgcaggg 5101 cgaagcccgg agagcaagcc cgtagggggG GCGCGCCCAG CTGTCTAGGG CGGCGGATTT 5161 GTCCTACTCA GGAGAGCGTT CACCGACAAA CAACAGATAA AACGAAAGGC CCAGTCTTTC 5221 GACTGAGCCT TTCGTTTTAT TTGATGCCT //