LOCUS pSEVA237M 3811 bp ds-DNA circular 08-MAY-2014 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers terminator complement(5336..5440) /label=T1 /ApEinfo_fwdcolor="#808080" /ApEinfo_revcolor="#808080" gene complement(3800..4462) /label=REP /ApEinfo_fwdcolor="#ff0080" /ApEinfo_revcolor="#ff0080" gene 2699..3514 /label=neo /ApEinfo_fwdcolor="#808080" /ApEinfo_revcolor="#808080" misc_feature complement(3546..3791) /label=oriT /ApEinfo_fwdcolor="#808040" /ApEinfo_revcolor="#808040" terminator 2469..2571 /label=T0 /ApEinfo_fwdcolor="#c0c0c0" /ApEinfo_revcolor="#c0c0c0" misc_feature 4463..5321 /note="oriV" misc_feature 3800..5321 /note="pBBR1 ori" CDS 2699..3514 /note="neomycin phosphotransferase" gene 1746..2462 /label=msfGFP /ApEinfo_fwdcolor="#008080" /ApEinfo_revcolor="#008080" CDS 1746..2462 /note="msfGFP" gene complement(9..950) /label=chnR promoter 1261..1656 /label=chnB promoter region PCR_primer 1237..1260 /label=R24 source 1..5441 /dnas_title="pSEVA2311M" ORIGIN 1 TTAATTAATC Aaaaaacaat agaggagact gaattttcag acacgagatt ggtgtgttcc 61 tgtctgattt cgatgggtgt tttcccaaaa aatttttgaa atactcgggt catatggctc 121 tgatcgcaaa aaccggcttc actggcaatc actttcagtg gaatttcttt ttttgaagtg 181 agcaaacgtt tagcaaattg caccctgaga ttcatgatgt aggcagcagg ggaattacca 241 aaatcgactt taaattttct cattaaatga ggcacgctca tcttgactaa tcccgctaaa 301 tcttcgaggg taattttaat gctcatgttt tcattgataa attctaataa cagtctgcgt 361 tgtaggggag taaaaccaga acggcactgt ccttccttaa aatctaattt ggcatactga 421 cggagtaaat gcagggcgat ctggttttta atgctctcta aatacaggtt tccacctaaa 481 ccaccttgct ttaattctag ttctaaaagt gaagtcagag caggtaaaac atgatcttcc 541 acaccgactt cgtctctgat tcggatcgaa gcgatatcat aatcaaacac cttattcgcc 601 attgaggtga tggagtcatg cgaaatgtag acatgggtga cggcaatatg gtcattccac 661 gcccagcggg aatcttcacc acaggtcaac aaggagacat aacccggctt aaccacttgg 721 gtatcccaag aggcatcgcc ttttctacgc atttccgccg cacttttttt atagttgacg 781 atcatgtagt cacgcatggc aggaatatgc acatcgaggt tggtatattc gtaccctctg 841 attgcaatgt tttcccaacc ggcattcatg ctgtcaattg tgacattacc cggaatccat 901 aacacgacat cttcgggttt gatcagcgta tttgctttgt ctgtgctCAT gctagctttt 961 tcctccttat aaagttaatc AACACCCCTT GTATTACTGT TTATGTAAGC AGACAGTTTT 1021 ATTGTTCATG ATGATATATT TTTATCTTGT GCAATGTAAC ATCAGAGATT TTGAGACACA 1081 AGACGTGGCA AAAAACATTA TCCAGAACGG GAGTGCGCCT TGAGCGACAC GAATTATGCA 1141 GTGATTTACG ACCTGCACAG CCATACCACA GCTTCCGATG GCTGCCTGAC GCCAGAAGCA 1201 TTGGTGCACC GTGCAGTCGA TGATAAGCTG TCAAACAGCG GATAACAATT TCACACAGGA 1261 gcaactaaaa gagattgttt ggatcagtta cccaaaatcg ttgaaaagat tttaactctt 1321 cgatttttat tttttaggta atcctagccc tctcgggggc taggattaaa aattttaagt 1381 tattccaaca cgaatgacaa attgttcaat gcaaaataaa aacatacaat atataaatat 1441 attttttaaA taaaacataa gattacaata aaataagaat ttttatttgg agtttgtttt 1501 ttttctacaa tgatcattat gtacaatttt taggttcacc ccatccaagc cttgtgattg 1561 cattcctgcg attctttatt caatgaataa gcaatgctat taatcagcaa tgaataacca 1621 gcaGtgcaga ttttgaataa attcacatgt cgtaatCCTA GGCCGCGGCC GCGCGAATTC 1681 GAGCTCGGTA CCCGGGGATC CTCTAGAGTC GACCTGCAGG CATGCAAGCT TAGGAGGAAA 1741 AACATAtgcg tAAAGGTGAA GAACTGTTCA CCGGTGTTGT TCCGATCCTG GTTGAACTGG 1801 ATGGTGATGT TAACGGCCAC AAATTCTCTG TTCGTGGTGA AGGTGAAGGT GATGCAACCA 1861 ACGGTAAACT GACCCTGAAA TTCATCTGCA CTACCGGTAA ACTGCCGGTT CCATGGCCGA 1921 CTCTGGTGAC TACCCTGACC TATGGTGTTC AGTGTTTTTC TCGTTACCCG GATCACATGA 1981 AGCAGCATGA TTTCTTCAAA TCTGCAATGC CGGAAGGTTA TGTACAGGAG CGCACCATTT 2041 CTTTCAAAGA CGATGGCACC TACAAAACCC GTGCAGAGGT TAAATTTGAA GGTGATACTC 2101 TGGTGAACCG TATTGAACTG AAAGGCATTG ATTTCAAAGA GGACGGCAAC ATCCTGGGCC 2161 ACAAACTGGA ATATAACTTC AACTCCCATA ACGTTTACAT CACCGCAGAC AAACAGAAGA 2221 ACGGTATCAA AGCTAACTTC AAAATTCGCC ATAACGTTGA AGACGGTAGC GTACAGCTGG 2281 CGGACCACTA CCAGCAGAAC ACTCCGATCG GTGATGGTCC GGTTCTGCTG CCGGATAACC 2341 ACTACCTGTC CACCCAGTCT AAACTGTCCA AAGACCCGAA CGAAAAGCGC GACCACATGG 2401 TGCTGCTGGA GTTCGTTACT GCcGCAGGTA TCACGCACGG CATGGATGAA CTCTACAAAT 2461 AAACTAGTCT TGGACTCCTG TTGATAGATC CAGTAATGAC CTCAGAACTC CATCTGGATT 2521 TGTTCAGAAC GCTCGGTTGC CGCCGGGCGT TTTTTATTGG TGAGAATCCA GGGGTCCCCA 2581 ATAATTACGA TTTAAATTTG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAA 2641 TATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT 2701 GAGCCATATT CAGCGTGAAA CGAGCTGTAG CCGTCCGCGT CTGAACAGCA ACATGGATGC 2761 GGATCTGTAT GGCTATAAAT GGGCGCGTGA TAACGTGGGT CAGAGCGGCG CGACCATTTA 2821 TCGTCTGTAT GGCAAACCGG ATGCGCCGGA ACTGTTTCTG AAACATGGCA AAGGCAGCGT 2881 GGCGAACGAT GTGACCGATG AAATGGTGCG TCTGAACTGG CTGACCGAAT TTATGCCGCT 2941 GCCGACCATT AAACATTTTA TTCGCACCCC GGATGATGCG TGGCTGCTGA CCACCGCGAT 3001 TCCGGGCAAA ACCGCGTTTC AGGTGCTGGA AGAATATCCG GATAGCGGCG AAAACATTGT 3061 GGATGCGCTG GCCGTGTTTC TGCGTCGTCT GCATAGCATT CCGGTGTGCA ACTGCCCGTT 3121 TAACAGCGAT CGTGTGTTTC GTCTGGCCCA GGCGCAGAGC CGTATGAACA ACGGCCTGGT 3181 GGATGCGAGC GATTTTGATG ATGAACGTAA CGGCTGGCCG GTGGAACAGG TGTGGAAAGA 3241 AATGCATAAA CTGCTGCCGT TTAGCCCGGA TAGCGTGGTG ACCCACGGCG ATTTTAGCCT 3301 GGATAACCTG ATTTTCGATG AAGGCAAACT GATTGGCTGC ATTGATGTGG GCCGTGTGGG 3361 CATTGCGGAT CGTTATCAGG ATCTGGCCAT TCTGTGGAAC TGCCTGGGCG AATTTAGCCC 3421 GAGCCTGCAA AAACGTCTGT TTCAGAAATA TGGCATTGAT AATCCGGATA TGAACAAACT 3481 GCAATTTCAT CTGATGCTGG ATGAATTTTT CTAATAATTA ATTGGACCGc ggtccgcgcg 3541 ttgtcCTTTT CCGCTGCATA ACCCTGCTTC GGGGTCATTA TAGCGATTTT TTCGGTATAT 3601 CCATCCTTTT TCGCACGATA TACAGGATTT TGCCAAAGGG TTCGTGTAGA CTTTCCTTGG 3661 TGTATCCAAC GGCGTCAGCC GGGCAGGATA GGTGAAGTAG GCCCACCCGC GAGCGGGTGT 3721 TCCTTCTTCA CTGTCCCTTA TTCGCACCTG GCGGTGCTCA ACGGGAATCC TGCTCTGCGA 3781 GGCTGGCCGT AGGCCGGCCC TACCGGCGCG GCAGCGTTAC CCGTGTCGGC GGCTCCAACG 3841 GCTCGCCATC GTCCAGAAAA CACGGCTCAT CGGGCATCGG CAGGCGCTGC TGCCCGCGCC 3901 GTTCCCATTC CTCCGTTTCG GTCAAGGCTG GCAGGTCTGG TTCCATGCCC GGAATGCCGG 3961 GCTGGCTGGG CGGCTCCTCG CCGGGGCCGG TCGGTAGTTG CTGCTCGCCC GGATACAGGG 4021 TCGGGATGCG GCGCAGGTCG CCATGCCCCA ACAGCGATTC GTCCTGGTCG TCGTGATCAA 4081 CCACCACGGC GGCACTGAAC ACCGACAGGC GCAACTGGTC GCGGGGCTGG CCCCACGCCA 4141 CGCGGTCATT GACCACGTAG GCCGACACGG TGCCGGGGCC GTTGAGCTTC ACGACGGAGA 4201 TCCAGCGCTC GGCCACCAAG TCCTTGACTG CGTATTGGAC CGTCCGCAAA GAACGTCCGA 4261 TGAGCTTGGA AAGTGTCTTC TGGCTGACCA CCACGGCGTT CTGGTGGCCC ATCTGCGCCA 4321 CGAGGTGATG CAGCAGCATT GCCGCCGTGG GTTTCCTCGC AATAAGCCCG GCCCACGCCT 4381 CATGCGCTTT GCGTTCCGTT TGCACCCAGT GACCGGGCTT GTTCTTGGCT TGAATGCCGA 4441 TTTCTCTGGA CTGCGTGGCC ATGCTTATCT CCATGCGGTA GGGgTGCCGC ACGGTTGCGG 4501 CACCATGCGC AATCAGCTGC AACTTTTCGG CAGCGCGACA ACAATTATGC GTTGCGTAAA 4561 AGTGGCAGTC AATTACAGAT TTTCTTTAAC CTACGCAATG AGCTATTGCG GGGGGTGCCG 4621 CAATGAGCTG TTGCGTACCC CCCTTTTTTA AGTTGTTGAT TTTTAAGTCT TTCGCATTTC 4681 GCCCTATATC TAGTTCTTTG GTGCCCAAAG AAGGGCACCC CTGCGGGGTT CCCCCACGCC 4741 TTCGGCGCGG CTCCCCCTCC GGCAAAAAGT GGCCCCTCCG GGGCTTGTTG ATCGACTGCG 4801 CGGCCTTCGG CCTTGCCCAA GGTGGCGCTG CCCCCTTGGA ACCCCCGCAC TCGCCGCCGT 4861 GAGGCTCGGG GGGCAGGCGG GCGGGCTTCG CCcTTCGACT GCCCCCACTC GCATAGGCTT 4921 GGGTCGTTCC AGGCGCGTCA AGGCCAAGCC GCTGCGCGGT CGCTGCGCGA GCCTTGACCC 4981 GCCTTCCACT TGGTGTCCAA CCGGCAAGCG AAGCGCGCAG GCCGCAGGCC GGAGGCTTTT 5041 CCCCAGAGAA AATTAAAAAA ATTGATGGGG CAAGGCCGCA GGCCGCGCAG TTGGAGCCGG 5101 TGGGTATGTG GTCGAAGGCT GGGTAGCCGG TGGGCAATCC CTGTGGTCAA GCTCGTGGGC 5161 AGGCGCAGCC TGTCCATCAG CTTGTCCAGC AGGGTTGTCC ACGGGCCGAG CGAAGCGAGC 5221 CAGCCGGTGG CCGCTCGCGG CCATCGTCCA CATATCCACG GGCTGGCAAG GGAGCGCAgc 5281 gaccgcgcag ggcgaagccc ggagagcaag cccgtagggg gGGCGCGCCC AGCTGTCTAG 5341 GGCGGCGGAT TTGTCCTACT CAGGAGAGCG TTCACCGACA AACAACAGAT AAAACGAAAG 5401 GCCCAGTCTT TCGACTGAGC CTTTCGTTTT ATTTGATGCC T //